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Expression Level of the Nos2 and Nfkb1 Genes in the Conditions of Esophageal Alkaline Burns and with the Administration of Melanin

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Abstract

In this study, an increase in the expression level of the proinflammatory Nos2 and Nfkb1 genes in blood and the esophageal mucus under the conditions of alkaline burns of the esophagus, degree 2 (AEB 2), has been shown. It has been found that the activity of endothelial nitric-oxide synthase (eNOS) and inducible nitric-oxide synthase (iNOS) increased, due to AEB 2, both in blood and the esophageal mucus. The introduction of melanin showed a decrease in the expression level of the Nos2 and Nfkb1 genes in blood and esophageal tissue compared with the AEB 2 variation indicators. A decrease, compared with the AEB 2 variation indicators, was also noted in the activity of eNOS and iNOS, indicating the presence of anti-inflammatory properties in this compound as well as a possibility to apply melanin as a substance promoting faster healing of esophageal chemical burns and preventing postburn complications.

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Funding

This study did not receive any particular grant from financial structures in the state, commercial, or noncommercial sectors.

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Authors and Affiliations

  1. Educational and Scientific Center—Institute of Biology and Medicine, Taras Shevchenko National University of Kyiv, 01601, Kyiv, Ukraine

    N. M. Chornenka, Ya. B. Raetska, A. S. Huet, T. V. Beregova, O. M. Savchuk & L. I. Ostapchenko

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  1. N. M. Chornenka
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  2. Ya. B. Raetska
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  3. A. S. Huet
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  4. T. V. Beregova
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  5. O. M. Savchuk
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  6. L. I. Ostapchenko
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Corresponding author

Correspondence to N. M. Chornenka.

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Conflict of interests. The authors declare that they have no conflict of interests.

Statement on the welfare of animals. The study was performed observing general ethical principles for animal experiments, which were adopted by the First National Congress of Ukraine on Bioethics (September 2001) and the international principles of the European Convention for protecting vertebral animals used for research and other scientific purposes as well as with observing other international agreements and national laws in this sphere.

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Translated by N. Tarasyuk

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Chornenka, N.M., Raetska, Y.B., Huet, A.S. et al. Expression Level of the Nos2 and Nfkb1 Genes in the Conditions of Esophageal Alkaline Burns and with the Administration of Melanin. Cytol. Genet. 55, 317–323 (2021). https://doi.org/10.3103/S0095452721040022

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  • DOI: https://doi.org/10.3103/S0095452721040022

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