Abstract
The systematic position of the Bacillus sp. 20F strain, an antagonist of phytopathogenic bacteria and micromycetes with expressed phosphate-mobilizing properties, was established. It was shown that the strain belongs to the group Bacillus subtilis based on its cultural-morphological and physiological-biochemical properties. The fatty acids of the cell wall of the strain are mainly branched derivatives of iso- and anteiso-C15:0 and C17:0 fatty acids (approximately 82%), characteristic for the species Bacillus amyloliquefaciens. According to the analysis of the nucleotide sequence of the 16S rRNA gene and the study of the profile of polymorphic nucleotides, the strain is classified as Bacillus velezensis.
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ACKNOWLEDGMENTS
We are grateful to M.A. Kharkhota (Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine) for assistance in sample preparation for chromato-mass spectrometric studies and M.S. Kharchuk (Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine) for assistance in sample preparation for electron microscopic examinations. Chromato-mass spectrometric and electron microscopic studies were performed using the equipment of the Center for Collective Use at Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine.
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Translated by V. Mittova
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Korzh, Y.V., Zelena, L.B., Dragovoz, I.V. et al. Taxonomic Analysis of Bacillus sp. 20F Strain, a Phosphate Mobilizer with Antagonistic Properties. Cytol. Genet. 55, 524–530 (2021). https://doi.org/10.3103/S0095452721060050
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DOI: https://doi.org/10.3103/S0095452721060050