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Development of SSR Markers for Psammochloa villosa (Trin.) Bor (Poaceae), a Dominant Species in the Inner Mongolian Plateau

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Abstract

We developed a set of SSR markers to investigate the genetic diversity and evolutionary history of Psammochloa villosa (Trin.) Bor, a dominant species of the Inner Mongolian Plateau and adjacent regions. We used sequenced transcriptomes of P. villosa to develop primer pairs for 97 SSR markers. Of these, we successfully amplified 46 (47%) SSRs and found that eleven were polymorphic among individuals. We tested these eleven SSRs in 122 accessions of P. villosa representing ten populations and determined that the number of alleles per locus ranged from one to seven, with an average of 2.727. The expected and observed heterozygosity per locus varied from 0 to 0.840 (mean: 0.471) and from 0 to 1.000 (mean: 0.679), respectively. The new SSR markers described in this study will be valuable for investigating the population genetic structure and history of P. villosa throughout its range and may help to develop hypotheses for understanding its evolutionary divergence from its closest relatives in the genus Stipa L.

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ACKNOWLEDGMENTS

The authors thank anonymous reviewers for their comments on the manuscript.

Funding

This study was supported by the National Natural Science Foundation of China (41761009, 31960052), the Natural Science Foundation of Qinghai Province (2019-ZJ-7011), “The Dawn of West China” Talent Training Program of the Chinese Academy of Sciences (2019_1_4), the Key Laboratory of Medicinal Animal and Plant Resources of the Qinghai-Tibet Plateau in Qinghai Province (2020-ZJ-Y40), which supported international collaboration with AJ Harris, the “High-end Innovative Talents Thousands of People Plan” in Qinghai Province and the “135 High-level Personnel Training Project” in Qinghai Province. All grants were made to Su.

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Authors and Affiliations

  1. Key Laboratory of Medicinal Animal and Plant Resources in the Qinghai-Tibetan Plateau, School of Life Sciences, Qinghai Normal University, 810008, Xining, China

    Yu-Ping Liu, Rui-Fang Liang, Ting Lv, Zi-Lan Ma, Tao Liu, Feng Liu & Xu Su

  2. Key Laboratory of Physical Geography and Environmental Process in Qinghai Province, Qinghai Normal University, 810008, Xining, China

    Yu-Ping Liu, Rui-Fang Liang, Ting Lv, Zi-Lan Ma, Tao Liu, Feng Liu & Xu Su

  3. Key Laboratory of Education Ministry of Earth Surface Processes and Ecological Conservation of the Qinghai-Tibet Plateau, Qinghai Normal University, 810008, Xining, China

    Yu-Ping Liu, Rui-Fang Liang, Ting Lv, Zi-Lan Ma, Tao Liu, Feng Liu & Xu Su

  4. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, 510650, Guangzhou, China

    AJ Harris

Authors
  1. Yu-Ping Liu
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  2. Rui-Fang Liang
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  3. Ting Lv
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  4. Zi-Lan Ma
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  5. Tao Liu
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  6. Feng Liu
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  7. Xu Su
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  8. AJ Harris
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Corresponding authors

Correspondence to Xu Su or AJ Harris.

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COMPLIANCE WITH ETHICAL STANDARDS

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

DATA ACCESSIBILITY

We deposited raw sequencing reads in the National Center for Biotechnology Information (NCBI) Sequence Read Archive (BioProject no. PRJNA730046). We also deposited sequence information for the developed primers in NCBI’s GenBank, and accession numbers are provided in Table 1.

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Liu, YP., Liang, RF., Lv, T. et al. Development of SSR Markers for Psammochloa villosa (Trin.) Bor (Poaceae), a Dominant Species in the Inner Mongolian Plateau. Cytol. Genet. 55, 576–582 (2021). https://doi.org/10.3103/S0095452721060086

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  • DOI: https://doi.org/10.3103/S0095452721060086

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