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Fluorimeter with Reflecting Optical Elements for Detecting of Simultaneous Reactions in a Microplate

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Optoelectronics, Instrumentation and Data Processing Aims and scope

Abstract

This paper describes the autocollimation optical scheme of a fluorimeter that allows detecting different reactions from multiple wells of a microplate at the same time. The image focusing is implemented a spherical mirror that excludes the presence of background illumination and chromatic aberration. The operation of the fluorimeter is demonstrated by measuring a signal from the microplate with 96 and 384 individual wells.

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References

  1. J. D. Robin, A. T. Ludlow, R. LaRanger, et al., “Comparison of DNA Quantification Methods for Next Generation Sequencing,” Sci. Rep. 6, 24067 (2016).

    Google Scholar 

  2. L. E. Morrison and L. M. Stols, “Sensitive Fluorescence-Based Thermodynamic and Kinetic Measurements of DNA Hybridization in Solution,” Biochemistry 32 (12), 3095–3104 (1993).

    Article  Google Scholar 

  3. E. D. Matayoshi, G. T. Wang, G. A. Krafft, and J. Erickson, “Novel Fluorogenic Substrates for Assaying Retroviral Proteases by Resonance Energy Transfer,” Science 247 (4947), 954–958 (1990).

    Article  ADS  Google Scholar 

  4. F. Shen, B. Sun, J. E. Kreutz, et al., “Multiplexed Quantification of Nucleic Acids with Large Dynamic Range Using Multivolume Digital RT-PCR on a Rotational SlipChip Tested with HIV and Hepatitis C Viral Load,” J. Amer. Chem. Soc. 133 (44), 17705–17712 (2011).

    Article  Google Scholar 

  5. V. P. Bessmeltsev, P. S. Zavyalov, V. P. Korolkov, et al., “Diffractive Focusing Fan-Out Element for the Parallel DNA Sequencer,” Avtometriya 53 (5), 48–56 (2017) [Optoelectron., Instrum. Data Process. 53 (5), 457–465 (2017)].

    Google Scholar 

  6. R. T. Hayden, Z. Gu, J. Ingersoll, et al., “Comparison of Droplet Digital PCR to Real-Time PCR for Quantitative Detection of Cytomegalovirus,” J. Clin. Microbiol. 51 (2), 540–546 (2013).

    Article  Google Scholar 

  7. Q. Xiang, B. Xu, and D. Li, “Miniature Real Time PCR on Chip with Multi-Channel Fiber Optical Fluorescence Detection Module,” Biomed Microdevices 9 (4), 443–449 (2007).

    Article  Google Scholar 

  8. C. T. Wittwer, K. M. Ririe, R. V. Andrew, et al., “The LightCycler: A Microvolume Multisample Fluorimeter with Rapid Temperature Control,” Biotechniques 22 (1), 176–181 (1997).

    Article  Google Scholar 

  9. Hard-ShellR 96-Well PCR Plates, High Profile, Semi Skirted, Black/White #hss9665. http://www.bio-rad.com/en-us/sku/hss9665-hard-shell-96-well-pcr-plates-high-profile-semi-skirted-black-white.

  10. Y. Tamaoki, T. Saga, T. Arai, and Y. Kikuchi, Pat. 7628958 US. Reaction Detecting Device Publ. 8 December 2009.

    Google Scholar 

  11. G. S. Landsberg, Elementary Physics Book. Vol. 3. Oscillations and Waves. Optics. Atomic and Nuclear Physics (Nauka, Moscow, 1985) [in Russian].

    Google Scholar 

  12. E. A. Iofis, Photographic Engineering (Sov. Entsikl., Moscow, 1981) [in Russian].

    Google Scholar 

  13. J. M. Geary, Introduction to Lens Design: With Practical Zemax Examples (Willmann-Bell, Virginia, 2002).

    Google Scholar 

  14. H. Kim, S. Dixit, Ch. J. Green, and G. W. Faris, “Nanodroplet Real-Time PCR System with Laser Assisted Heating,” Opt. Express. 17 (1), 218–227 (2009).

    Article  ADS  Google Scholar 

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Correspondence to V. P. Chubakov.

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Original Russian Text © V.P. Chubakov, P.A. Chubakov, 2018, published in Avtometriya, 2018, Vol. 54, No. 5, pp. 73–78.

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Chubakov, V.P., Chubakov, P.A. Fluorimeter with Reflecting Optical Elements for Detecting of Simultaneous Reactions in a Microplate. Optoelectron.Instrument.Proc. 54, 484–489 (2018). https://doi.org/10.3103/S8756699018050096

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  • DOI: https://doi.org/10.3103/S8756699018050096

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