Elsevier

Pathology

Volume 18, Issue 3, 1986, Pages 313-320
Pathology

Production and characterization of monoclonal antibodies reactive with subsets of epidermal cells

https://doi.org/10.3109/00313028609059483Get rights and content

Summary

Monoclonal antibodies were raised against an extract of human callus to provide markers of cell differentiation. Three antibodies, LMM1, LMM2 and LMM3, have been isolated and react with different histological structures in skin. LMM1 reacts with two distinct proteins of 62-65 kD molecular weight and stains cells in the stratum spinosum and stratum granulosum of normal epidermis. This antibody stains a high proportion of cultured epidermal cells showing a diffuse cytoplasmic reactivity with nucleolar staining also being present in some cells. LMM2 reacts with several lower molecular weight proteins, the two major bands being 38 kD and 44 kD. It stains basal epidermal cells strongly and stratum spinosum cells less strongly. A population of cultured kératinocytes reacts with an intense fibre stain suggesting that this antibody is directed against an intermediate filament or intermediate filament-associated protein. LMM3 reacts with a 48 kD protein. Although it does not stain cells in normal epidermis its staining of a subpopulation of hair follicle cells is consistent with this protein being a cytokeratin. It also stains a population of cultured kératinocytes, the main reactivity being against mitotic cells. The anti-cytokeratin or anti-cytokeratin-associated reactivity of these three antibodies is supported by their altered reactivities against psoriasis epidermis, where modifications of cytokeratin biosynthesis are known to occur.

References (30)

  • G. Matalanis et al.

    Lecting binding patterns and monoclonal antibodies to epidermal antigens in tumours of the skin

    Pathology

    (1986)
  • R.M. Lavker et al.

    Heterogeneity in epidermal basal ketatinocytes: morphological and functional correlations

    Science

    (1982)
  • T.-T. Sun et al.

    Keratin cytoskeletons in epithelial cells of internal organs

    Proc Natl Acad Sci USA

    (1979)
  • E. Lazarides

    Intermediate filaments as mechanical integrators of cellular space

    Nature

    (1980)
  • T.H. Loening et al.

    Keratin polypeptide distribution in normal and diseased human epidermis and oral mucosa

    Virchows Arch A

    (1980)
  • Cited by (12)

    View all citing articles on Scopus
    View full text