The fortification of milk with phytosterols is an increasingly common practice to enhance the sterol profile and offer consumers potential health benefits. This study investigated whether cattle feed can influence the profile of phytosterols and cholesterol in the milk produced as an alternative to direct fortification of milk. Five experiments were performed using feeds commonly used by Australian dairy farmers and selected formulated rumen-protected feeds. Statistical significances were observed for some individual plant sterols and cholesterol in milk under these differing feeding regimens compared with the respective controls. In the case of the phytosterols, where the daily recommended consumption is typically 2 g per day, the total phytosterols were <0.12 mg/100 mL of milk. An experiment using a rumen-protected feed with high phytosterol levels suggested a decreased transfer of cholesterol to the milk by as much as 20%, although further work is required to confirm these preliminary results. Overall, the study suggests that different feeding practices have minimal effect on the resulting sterol profile of the milk.
