Micropropagation of Clerodendrum serratum L. through Direct and Indirect Organogenesis

Authors

  • SM Vidya Department of Biotechnology, NMAM Institute of Technology, Nitte, Karkala, Udupi Dist., Karnataka-574 110
  • V Krishna Department of PG Studies and Research in Biotechnology and Bioinformatics, Jnanasahyadri, Kuvempu University, Shimoga, Karnataka-577 451
  • BK Manjunatha Department of Biotechnology, Oxford College of Engineering, Bommanahalli, Bangalore, Karnataka-560 068
  • MR Pradeepa Department of Biotechnology, NMAM Institute of Technology, Nitte, Karkala, Udupi Dist., Karnataka-574 110

DOI:

https://doi.org/10.3329/ptcb.v22i2.14208

Keywords:

Clerodendrum serratum, Micropropagation, LM media, Microshoots

Abstract

In vitro clonal propagation of Clerodendrum serratum L., a rare medicinal plant has been reported by using LM medium supplemented with different growth hormones. The maximum number of shoots with maximum length were obtained from stem derived callus on LM media fortified with 1.5 mg/l BAP and 0.3 mg/l NAA. Nodal explants showed direct organogenesis on LM media containing BAP (0.5 mg/l) alone. The regenerated shoots were successfully rooted with maximum frequency (100%) on half strength LM media supplemented with 0.5 mg/l NAA. The well rooted microshoots were successfully transferred to hardening and survival rate was 88%.

DOI: http://dx.doi.org/10.3329/ptcb.v22i2.14208

Plant Tissue Cult. & Biotech. 22(2): 179-185, 2012 (December)

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Published

2013-03-19

How to Cite

Vidya, S., Krishna, V., Manjunatha, B., & Pradeepa, M. (2013). Micropropagation of Clerodendrum serratum L. through Direct and Indirect Organogenesis. Plant Tissue Culture and Biotechnology, 22(2), 179–185. https://doi.org/10.3329/ptcb.v22i2.14208

Issue

Vol. 22 No. 2 (2012)

Section

Articles