Effects of Some Local Anesthetics on Ca⁺⁺ Binding to Lipid-Extracted RBC Membrane, Egg Albumin Film and Filter Paper

Local anesthetics at a concentration of 10 mM(procaine and lidocaine) were found to inhibit competitively Ca⁺⁺ binding to lipidextracted RBC membrane, and also to egg albumin film fixed on cover glasses or impregnated into Whatman filter paper (No. 1).

A competitive inhibition by local anesthetics was also found in Ca⁺⁺ binding to Whatman filter paper which had been pretreated with organic solvents to extract possible contaminated lipids. Therefore, it is suggested that the local anesthetics inhibit Ca⁺⁺ binding not only to phospholipids but to some macromolecules such as membrane proteins, egg albumin film and filter paper.