Delivering Singlet Oxygen in Dark Condition With an Anthracene-Functionalized Semiconducting Compound for Enhanced Phototheranostics
Jian Shen1 Liuhong Pan2 Xujing Zhang2 Zhenyuan Zou2 Bo Wei3 Yongchang Chen1* Xiaoyan Tang3* 
Dengfeng Zou2*- 1Department of Urology, Changshu No. 2 People’s Hospital, Changshu, China
- 2School of Pharmacy, Guilin Medical University, Guilin, China
- 3Department of Materials Engineering, Changshu Institute of Technology, Changshu, China
Photodynamic therapy (PDT) utilizes the photogeneration of reactive oxygen species (ROS) with high cytotoxicity to kill cancer cells, holding great promise for cancer treatment. Fractionated delivery of singlet oxygen (1O2) is a wise approach to relieving hypoxia, thus enhancing the therapeutic efficacy. In this article, an anthracene-functionalized semiconducting compound (DPPA) has been designed and synthesized. With irradiation, the compound is able to undergo efficient intersystem crossing (ISC) and non-radioactive decay for photodynamic/photothermal synergistic therapy. In addition, the anthracene module is able to capture and release 1O2 reversibly with or without irradiation. DPPA nanoparticles (NPs) obtained by nanoprecipitation with DSPE-PEG exhibit considerable high phototoxicity on human kidney cancer cells (A498), and the half maximum inhibitory concentration (IC50) is 15.8 μg/ml. Furthermore, an in vivo study demonstrates that complete tumor suppression was observed when the mice were administered DPPA NPs with the help of laser, compared with the control and dark groups. The H&E analysis of the normal tissues (the heart, liver, spleen, lungs, and kidney) indicates that such NPs cause no side effects, indicating the biosafety of DPPA NPs. The results provide a strategy to design a heavy-atom–free photosensitizer for photothermal and fractionated PDT against kidney tumors.
Introduction
With the increasing cases of cancer worldwide, the development of new therapeutic methods for cancer treatment is of tremendous significance. (Siegel et al., 2021). Phototherapy utilizes the photogeneration of cytotoxic reactive oxygen species (ROS) (Xu et al., 2017; Zhen et al., 2017; Huang et al., 2018; Li et al., 2019a; Liu et al., 2019a; Li et al., 2019b; Meng et al., 2019; Li et al., 2020; Xiao et al., 2020; Yang et al., 2020; Yao et al., 2020; Zheng et al., 2020; Zou et al., 2021a; Zou et al., 2021b; Wang et al., 2021) or heat (Chen et al., 2019; Li et al., 2019c; Ma et al., 2019; Zhang et al., 2019; Zhao et al., 2020) to induce cell apoptosis and further leads to tumor suppression, holding great promise for cancer treatment. (Zhou et al., 2016; Ng et al., 2018; Liu et al., 2019b). However, in the photoinduced ROS generation, especially the oxygen-dependent type II process, continuous irradiation will inevitably cause hypoxia, which will, in turn, reduce the oxygen supply and diminish the therapeutic efficacy. (Fan et al., 2016). Therefore, hypoxia is acknowledged as the obstacle of photodynamic therapy (PDT).
Continuous irradiation of the tumor leads to the burst release of ROS and induces tumor hypoxia, which is disadvantageous for cancer treatment. Fractionated delivery of singlet oxygen in the dark environment may be a wise strategy to enhance the therapeutic efficacy. (Turan et al., 2016; Zhu et al., 2019; Zou et al., 2020). It is considered as a mild PDT process, resulting in the diminished blood vessel damage and providing enough time for the oxygen supply in the blood circulation. Anthracene derivatives are capable of capturing singlet oxygen to form an endoperoxide intermediate by a cycloaddition reaction with laser irradiation. Furthermore, in the dark cycle, the endoperoxide will reversibly release 1O2 to regenerate the anthracene modules. (Wang and Zhao, 2017; Filatov et al., 2018; Zhu et al., 2019). In addition, anthracene derivatives are usually considered as heavy-atom–free compounds for efficient intersystem crossing (ISC), and the dark toxicity may be quenched. (Filatov et al., 2017; Callaghan et al., 2019). Considerable attention has been attached to semiconducting compounds due to their unique photophysical and photochemical properties. (Chen et al., 2016; Tang et al., 2018; Li et al., 2019d; Shen et al., 2019; Deng et al., 2020; Zhang et al., 2021). For example, Chen et al. designed a heavy-atom-free compound for efficient singlet oxygen generation and continuous PDT. (Zou et al., 2020). Another example is that Pu et al. designed a semiconducting polymer for PDT-induced immunotherapy. (Li et al., 2019e).
In this work, we have designed and prepared a heavy-atom-free semiconducting compound 3,6-bis[5-(anthracen-9-yl)furan-2-yl]-2,5-bis(2-octyldodecyl)pyrrolo [3,4-c]pyrrole-1,4(2H,5H)-dione (denoted as DPPA) by a C-H activation reaction (Scheme). Compared with the standard substance methylene blue (MB), the singlet oxygen quantum yield (1O2 QY) of the as-obtained DPPA is 21.3% in dichloromethane (DCM). DPPA nanoparticles (NPs) obtained by nanoprecipitation exhibit spherical morphology with an average diameter of 52 nm. Such NPs are able to capture singlet oxygen with irradiation and release it in the dark condition. The photothermal conversion efficiency of DPPA NPs is 35.6%. The PDT and PTT synergistic effect may promise the excellent therapeutic efficacy of DPPA NPs. (Wang et al., 2019; Wu et al., 2019; Chang et al., 2020; Xu et al., 2020). In vitro MTT assay indicates the half maximum inhibitory concentration (IC50) of DPPA NPs is as low as 15.8 μg/ml in human kidney cells (A498) with laser irradiation. Further fluorescence imaging in vivo suggests that DPPA NPs are able to passively target the tumor by the EPR (enhanced permeability and retention) effect. With the help of a laser, DPPA NPs are capable of inhibiting the tumor growth while exerting little side effects on normal tissues, including the heart, spleen, liver, kidney, and lungs. The results suggest that DPPA NPs have great potential for photothermal and fractionated photodynamic therapy.
Experimental Section
Materials and Apparatus
1H NMR and 13C NMR spectra were performed on a Bruker DRX NMR spectrometer in CDCl3 (δ = 7.26 ppm) at 298 K as the internal standard. UV-vis and fluorescence spectra were measured on a Shimadzu spectrophotometer, from Japan, (UV-3600) and a HITACHI spectrometer (F-4600, Japan), respectively. TEM of the nanoparticles were measured on equipment (JEOL JEM-2100). Dynamic light scattering (DLS) of DPPA NPs was tested on a particle size analyzer (90 Plus, Brookhaven Instruments, United States). Fluorescence imaging of DPPA NPs in nude mice was recorded on an IVIS spectrum.
Synthesis and Characterization of DPPA
A mixture of DPP (200.0 mg, 0.24 mmol), 9-bromoanthracence (160.0 mg, 0.60 mmol), pivalic acid (20 mg, 0.20 mmol), Pt (OAc)2 (11.0 mg, 0.02 mmol), and K2CO3 (83.0 mg, 0.60 mmol) was dissolved in 5 ml DMA (N,N-dimethyl acetamide). Then, N2 was bubbled to drive off possible oxygen and water in the system. The mixture was heated to 110°C under the protection of N2 gas for 12 h. After cooling to room temperature, the mixture was poured into saturated sodium chloride solution (150 ml) and extracted with dichloromethane (100 ml) three times. The organic layer was washed with brine, followed by drying with anhydrous sodium sulfate. The solvent was removed by rotary evaporation and purified by silica gel column chromatography with dichloromethane and hexane (1: 2, v/v) as the developing solvent. Dark blue solids were obtained (Yield: 95 mg, 30%). 1HNMR: δ H 8.60-8.56 (2H, m), 8.55-8.51 (4H, d), 8.22-8.17 (4H, d), 8.12-8.01 (4H, m), 7.76-7.66 (4H, m), 4.36-4.23 (4H, d), 2.12-2.02 (2H, s), 1.33-1.21 (50H, m), and 0.96-0.76 (26H, m). 13CNMR: 160.12, 143.83, 128.53, 127.70, 125.67, 124.55, 115.38, 57.13, 45.73, 37.35,30.84, 25.07, 21.63, 17.31, and 13.09. MS: m/z: 1,180.80, found: 1,181.85.
Cell Culture and MTT Assay
At 37°C, human kidney cancer (A498) cells were cultured with a medium consisting of 12% fetal bovine serum (FBS) in DMEM (Gibico) under the atmosphere of 5% CO2. DPPA NPs with different concentrations were co-cultivated with A498 cells in the 96-well plate. For the illumination group, each well was irradiated with a 660 nm laser for 8 min. In contrast, the wells in the control and no illumination groups have not been irradiated. Relative cell viability was determined by recording the absorbance of MTT [3-(4,5-dimethylthiazol- 2-yl)-2,5- diphenyltetrazolium bromide]. MTT in PBS (5 mg/ml) was added to the well (20 μl) and then incubated for 4 h. After that, the mother liquid was discarded, and DMSO (200 μl) was added. The absorbance of each well was recorded on a Bio-Tek microplate reader. Cell viability was then calculated according to the equation:
Cell viability (%) = mean absorbance of the group incubated with DPPA NPs/mean absorbance of the group.
All the cell experiments were repeated three times.
Cellular Uptake and Fluorescence Imaging of Cellular ROS
A498 cells were cultured with DPPA NPs (3 ml) in a confocal dish for 4 h. Then, the medium was discarded, and the cells were washed with PBS (1 ml, 3 times), followed by the co-culture with 1 ml polyoxymethylene for 25 min. Then, polyoxymethylene was discarded, and the cells were also washed with PBS three times (1 ml). The cells were further co-cultivated with DCF-DA (2,7-dichlorodihydrofluorescein diacetate, 10 µmol) for 5 min, followed by washing with PBS (1 ml) three times. A 660 nm laser was then applied to the sample for 3 min (0.5 W/cm2). The cells were excited at 633 nm, and fluorescence was observed from 650 to 750 nm to investigate the cellular uptake. They were excited with a 488 nm laser, and fluorescence was observed from 490 to 560 nm to show the ROS generation.
Fluorescence Imaging- and Photothermal Imaging-Guided Phototherapy
The procedure follows the rules of the National Institutes of Health (NIH). The animal study was approved by Guilin Medical University (SCXK 2007-001). A total of 15 nude mice were purchased and then inoculated with A498 cells. Three mice have been chosen to perform in vivo fluorescence imaging. The fluorescence image was captured first, and then, three mice were intravenously injected with DPPA NPs, and the fluorescence imaging pictures were also captured at different time points. A total of 12 nude mice were divided into three groups at random when the tumor volume reached about 80 mm3. For the dark and illumination groups, the mice were intravenously injected DPPA NPs (200 μg/ml, 100 μL). After 12 h, the tumors of the PBS + laser and DPPA + laser groups were irradiated by a 660 nm laser (1 W/cm2) for 8 min, while the mice in the DPPA-only group were not irradiated. These nude mice were then sacrificed for histology analysis.
Statistical Analysis
All numeric data are expressed as mean ± s.d., unless otherwise indicated. The significance between two groups was analyzed by the two-tailed Student’s t-test. Statistical analysis was performed by GraphPad Prism 6.0. p values of less than 0.05 were considered significant (*p < .05, **p < .01, ***p < .001).
Results and Discussion
Synthesis and Generation Characterization of DPPA and NPs
DPPA was prepared and characterized by 1HNMR, 13CNMR, and mass spectroscopy (Supplementary Figure S1–S3). DPPA NPs were characterized by the UV-vis and fluorescence emission spectra. DPPA shows narrow absorption peaks at 522 and 558 nm in DCM, while the emission peaks were shifted to 593 and 629 nm, respectively, indicating their responsiveness to near-infrared (NIR) light. (Figures 1A,B). A large Stokes shift was observed for the absorbance of the maximum absorbance of DPPA NPs in water (616 and 719 nm), which is attributed to both the solvent effect and the aggregation of DPPA NPs in aqueous solution. This phenomenon could also be found of other photosensitizers. (Zou et al., 2020). The morphology characterized by a transmission electron microscope (TEM) suggests DPPA is self-assembled to form uniform NPs (Figure 1C), consisting with the dynamic light-scattering (DLS) result (mean diameter ∼52 nm) (Figure 1D).
FIGURE 1. Normalized (A) absorbance spectra of DPPA. (B) fluorescence spectra of DPPA in THF and NPs in water. (C) TEM of DPPA NPs. (D) DLS of DPPA NPs in water.
Singlet Oxygen Generation, Reversible Capture and Release, and Photothermal Conversion Efficiency
For an ideal photosensitizer, high singlet oxygen quantum yield (1O2 QY) promises excellent phototherapeutic efficacy. Therefore, the 1O2 QY of DPPA was calculated by recording the absorbance of 1,3-diphenylisobenzofuran (DPBF) with laser irradiation. With methylene blue (MB ∼Φ = 57%) as the standard substance in DCM (Supplementary Figure S4), the absorbance of DPBF kept decreasing with irradiation while that of DPPA remained unchanged, and the 1O2 QY is calculated as 21.3% (Figures 2A,B). It is worth noting that DPPA is heavy-atom-free, and this may reduce the potential dark toxicity itself. Then, nanoprecipitation was used to prepare DPPA NPs with good dispersity in water. The singlet oxygen generation ability of DPPA NPs was measured using singlet oxygen sensor green (SOSG) as an indicator. It can be found that DPPA NPs inherit the high 1O2 generation ability as the fluorescence enhancement of SOSG was enhanced by 3.2 times with irradiation (Figure 2C). However, the singlet oxygen generation ability of DPPA NPs is lower than that of Rose Bengal (Supplementary Figure S6). The 1O2 capture and release was, then, also characterized by recording the fluorescence intensity of SOSG with or without irradiation. After irradiation for 1 min, the intensity was enhanced two times. It continued to increase even without laser irradiation, indicating the fractionated delivery of 1O2 in the dark environment (Figure 2D).
FIGURE 2. (A) 1O2 generation of DPPA in DCM using DPBF as a probe. (B) Linear fitting of time versus absorbance. (C) Fluorescence intensity SOSG with laser irradiation (660 nm, 50 W/cm2). (D) Fluorescence intensity of SOSG with irradiation for 1 min and then without irradiation (660 nm, 50 W/cm2). (E) Temperature elevation and decrease curve of DPPA NPs (660 nm, 500 W/cm2, 10 min). (F) Linear fitting of −lnθ versus time.
High photothermal conversion efficiency promises the photosensitizer with a high photothermal therapeutic efficacy. The heating curve of DPPA NPs in distilled water with irradiation or the cooling curve without irradiation was recorded. (Figure 2E). The temperature elevation of 25.2 C with laser irradiation in the presence of DPPA is much higher than that of water under the same condition (5.1 C) with a high photothermal conversion efficiency of 35.6% (Figure 2F). Such NPs show excellent photostability because no obvious decay was observed, regardless of irradiation (Supplementary Figure S5).
Cellular Uptake, 1O2 Generation, and MTT Assay In Vitro
Based on the singlet oxygen detection and photothermal conversion efficiency investigation, we then evaluated the therapeutic efficacy of DPPA NPs in vitro. The cellular uptake and singlet oxygen generation ability of DPPA NPs were investigated by confocal laser scanning microscopy (CLSM). DPPA NPs are able to be uptaken by human kidney cells (A498) after incubation for 6 h (Figure 3A). With laser irradiation, singlet oxygen generation could be observed due to the strong green fluorescence. (Figure 3A). After incubation for 24 h, two groups were divided to investigate the dark or photo toxicity by MTT assay. For the dark group, the cell viability remained very high, regardless of the concentration, indicating the low dark toxicity of DPPA NPs (Figure 3B). In comparison, the cells’ viability with irradiation show concentration-dependent death, and the half maximum inhibitory concentration of DPPA NPs is 15.8 μg/ml (Figure 3B). The results demonstrate that DPPA NPs have a potential for PDT/PTT synergistic therapy.
FIGURE 3. (A) In vitro cellular uptake of DPPA NPs in A498 cells and ROS generation in the presence of DCF-DA as a probe (660 nm, 100 W/cm2). (B) Cell viability of A498 cells in the presence of DPPA NPs at different concentrations (0, 5, 10, 15, 20, and 25 μg/ml) (660 nm, 100 W/cm2, 8 min).
In Vivo Fluorescence Imaging-Guided Photothermal and Fractionated Photodynamic Therapy
Since DPPA NPs exhibit considerable cytotoxicity in vitro, we then further investigated the phototherapeutic efficacy in vivo. Photothermal and fluorescence imaging were investigated (Figures 4A,B). A total of 15 nude female mice bearing A498 tumor were used in this study. When the tumor volume reached 200 mm3, biodistribution was determined by fluorescence imaging in vivo after an intravenous injection of DPPA NPs. Time-dependent fluorescence images of the nude mice were captured (Figure 4A). After injection with DPPA NPs for 8 h, the fluorescence intensity of the tumor reached the peak, indicating 8 h is the most appropriate time point for laser performance. After 24 h, these mice were sacrificed. Then, the ex vivo fluorescence intensities of the tumor, heart, liver, spleen, lungs, and kidney were recorded (Figure 4C). The fluorescence intensity of the tumor remained the strongest after injection with DPPA NPs for 24 h (Figure 4D). For photothermal imaging, a significant temperature elevation of 16 C was observed for the tumor with laser irradiation for 8 min. In contrast, temperature elevation of the control group is only 4.0 C (Figures 4B,E), which indicates that DPPA NPs show outstanding photothermal efficacy in vivo.
FIGURE 4. (A) Time-dependent fluorescence imaging of A498 tumor with intraveneous injection of DPPA NPs. (B) Photothermal imaging of the mouse with an injection of PBS and DPPA NPs (660 nm, 500 W/cm2, 8 min). (C) Quantification of the tumor intensity at different time points. (D) Quantification of the main organs and tumor. (E) Tumor temperature change of the PBS + laser and DPPA + laser groups.
The tumor volume of the mice administered with DPPA NPs is parallel to that of the PBS + laser group, suggesting the low dark toxicity of such NPs (Figure 5A). Another piece of evidence is all the mice tend to gain more weight in the three groups, regardless of irradiation or not (Figure 5B). However, tumor proliferation has been suppressed proportionally after laser treatment. After treatment for 3 times, the tumors of the DPPA NPs + laser group completely disappeared, demonstrating the phototherapeutic efficacy of DPPA NPs (Figure 5A). After treatment, the mice were still raised to observe the survival (Figure 5C). Mice in the DPPA NPs + laser group still remained alive while those in the PBS + laser and DPPA NPs-only groups suffered from low survival. Representative mice in the PBS + laser, DPPA NPs-only, and DPPA NPs + laser groups are shown in Supplementary Figure S6. The H&E stained pictures of the tumor in the three groups are very similar with a healthy nucleus (Figure 5D).
FIGURE 5. (A) Relative tumor volume of the mice in PBS + laser, DPPA-only, and DPPA + laser groups (660 nm, 500 W/cm2, 8 min). (B) Body weight change. (D) H&E stained pictures of the tumor in the (C) PBS + laser and (D) DPPA-only groups. Scale bar: 10 μm.
After treatment, all the mice were sacrificed, and the normal organs were collected for the H&E study. No obvious difference was observed in H&E stained pictures (Figure 6). All the results demonstrated that DPPA NPs exhibit strong antitumor activity and low side effects in vivo, suggesting their good biocompatibility.
FIGURE 6. H&E pictures of the heart, liver, lungs, kidney, and spleen in the PBS + laser, DPPA-only, and DPPA + laser groups. Scale bar: 10 μm.
Conclusion
In summary, an anthracene-functionalized semiconducting photosensitizer DPPA has been designed and prepared with a high 1O2 QY of 21.3%. The anthracene module acts as a store for fractionated delivery of singlet oxygen because such a module is able to undergo the cycloaddition reaction to store singlet oxygen with laser irradiation while releasing it without irradiation. The as-prepared DPPA NPs still retain the 1O2 generation ability and simultaneously the high photothermal conversion efficiency (35.6%). MTT assay shows that DPPA NPs show quite low dark toxicity but high phototoxicity with a low IC50 of 15.8 μg/ml. In vivo photothermal- and fluorescence imaging-guided phototherapy suggest that such NPs are capable of suppressing the tumor growth at a low dose but cause no damage to normal tissues, suggesting the biocompatibility. These results provide some insights to design semiconducting photosensitizers with high phototoxicity, low dark toxicity, and good biocompatibility for photothermal and fractionated photodynamic therapy.
Data Availability Statement
The original contributions presented in the study are included in the article/Supplementary Material; further inquiries can be directed to the corresponding authors.
Ethics Statement
The animal study was reviewed and approved by Guilin Medical University (SCXK2007-001).
Author Contributions
JS, YC, XT, and DZ designed this project and conceived the study. JS synthesized, characterized the materials, and wrote the manuscript. LP, XZ, and ZZ helped do the cell and mice experiment. BW rechecked the data.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s Note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors, and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Acknowledgments
The authors acknowledge financial support from the Scientific Research Project from the Second Hospital of Changshu (No. CSEY2021037) and the Six Talent Peaks Project of Jiangsu Province (No. 2018-SWYY-001).
Supplementary Material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fbioe.2022.781766/full#supplementary-material
References
Callaghan, S., Filatov, M. A., Savoie, H., Boyle, R. W., and Senge, M. O. (2019). In Vitro cytotoxicity of a Library of BODIPY-Anthracene and -pyrene Dyads for Application in Photodynamic Therapy. Photochem. Photobiol. Sci. 18 (2), 495–504. doi:10.1039/c8pp00402a
Chang, M., Hou, Z., Wang, M., Wang, M., Dang, P., Liu, J., et al. (2020). Cu2 MoS4/Au Heterostructures with Enhanced Catalase-like Activity and Photoconversion Efficiency for Primary/Metastatic Tumors Eradication by Phototherapy-Induced Immunotherapy. Small 16 (14), e1907146. doi:10.1002/smll.201907146
Chen, Q., Hu, Q., Dukhovlinova, E., Chen, G., Ahn, S., Wang, C., et al. (2019). Photothermal Therapy Promotes Tumor Infiltration and Antitumor Activity of CAR T Cells. Adv. Mater. 31 (23), e1900192. doi:10.1002/adma.201900192
Chen, Q., Xu, L., Liang, C., Wang, C., Peng, R., and Liu, Z. (2016). Photothermal Therapy with Immune-Adjuvant Nanoparticles Together with Checkpoint Blockade for Effective Cancer Immunotherapy. Nat. Commun. 7, 13193. doi:10.1038/ncomms13193
Deng, J., Zhong, N., Zhang, X., Li, C., Xu, C., and Zhao, J. (2020). A Carbazole Functionalized Semiconducting Compound as a Heavy Atom Free Photosensitizer for Phototherapy against Lung Cancer. J. Mater. Chem. B 8 (47), 10764–10769. doi:10.1039/d0tb02314k
Fan, W., Huang, P., and Chen, X. (2016). Overcoming the Achilles' Heel of Photodynamic Therapy. Chem. Soc. Rev. 45 (23), 6488–6519. doi:10.1039/c6cs00616g
Filatov, M. A., Karuthedath, S., Polestshuk, P. M., Callaghan, S., Flanagan, K. J., Telitchko, M., et al. (2018). Control of Triplet State Generation in Heavy Atom-free BODIPY-Anthracene Dyads by media Polarity and Structural Factors. Phys. Chem. Chem. Phys. 20 (12), 8016–8031. doi:10.1039/c7cp08472b
Filatov, M. A., Karuthedath, S., Polestshuk, P. M., Savoie, H., Flanagan, K. J., Sy, C., et al. (2017). Generation of Triplet Excited States via Photoinduced Electron Transfer in Meso-Anthra-BODIPY: Fluorogenic Response toward Singlet Oxygen in Solution and In Vitro. J. Am. Chem. Soc. 139 (18), 6282–6285. doi:10.1021/jacs.7b00551
Huang, H. C., Pigula, M., Fang, Y., and Hasan, T. (2018). Immobilization of Photo-Immunoconjugates on Nanoparticles Leads to Enhanced Light-Activated Biological Effects. Small, 1800236–1800246. doi:10.1002/smll.201800236
Li, J., Cui, D., Jiang, Y., Huang, J., Cheng, P., and Pu, K. (2019). Near-Infrared Photoactivatable Semiconducting Polymer Nanoblockaders for Metastasis-Inhibited Combination Cancer Therapy. Adv. Mater. 31 (46), e1905091. doi:10.1002/adma.201905091
Li, J., Cui, D., Huang, J., He, S., Yang, Z., Zhang, Y., et al. (2019). Organic Semiconducting Pro‐nanostimulants for Near‐Infrared Photoactivatable Cancer Immunotherapy. Angew. Chem. Int. Ed. 58 (36), 12680–12687. doi:10.1002/anie.201906288
Li, J., Huang, J., Lyu, Y., Huang, J., Jiang, Y., Xie, C., et al. (2019). Photoactivatable Organic Semiconducting Pro-nanoenzymes. J. Am. Chem. Soc. 141 (9), 4073–4079. doi:10.1021/jacs.8b13507
Li, L., Zou, J., Dai, Y., Fan, W., Niu, G., Yang, Z., et al. (2020). Burst Release of Encapsulated Annexin A5 in Tumours Boosts Cytotoxic T-Cell Responses by Blocking the Phagocytosis of Apoptotic Cells. Nat. Biomed. Eng. 4 (11), 1102–1116. doi:10.1038/s41551-020-0599-5
Li, Y., Li, X., Doughty, A., West, C., Wang, L., Zhou, F., et al. (2019). Phototherapy Using Immunologically Modified Carbon Nanotubes to Potentiate Checkpoint Blockade for Metastatic Breast Cancer. Nanomedicine: Nanotechnology, Biol. Med. 18, 44–53. doi:10.1016/j.nano.2019.02.009
Li, Z., Hu, Y., Fu, Q., Liu, Y., Wang, J., Song, J., et al. (2019). NIR/ROS‐Responsive Black Phosphorus QD Vesicles as Immunoadjuvant Carrier for Specific Cancer Photodynamic Immunotherapy. Adv. Funct. Mater. 30 (3), 1905758–1905766. doi:10.1002/adfm.201905758
Liu, X., Li, M., Han, T., Cao, B., Qiu, Z., Li, Y., et al. (2019). In Situ Generation of Azonia-Containing Polyelectrolytes for Luminescent Photopatterning and Superbug Killing. J. Am. Chem. Soc. 141, 11259–11268. doi:10.1021/jacs.9b04757
Liu, Y., Bhattarai, P., Dai, Z., and Chen, X. (2019). Photothermal Therapy and Photoacoustic Imaging via Nanotheranostics in Fighting Cancer. Chem. Soc. Rev. 48 (7), 2053–2108. doi:10.1039/c8cs00618k
Ma, Y., Zhang, Y., Li, X., Zhao, Y., Li, M., Jiang, W., et al. (2019). Near-Infrared II Phototherapy Induces Deep Tissue Immunogenic Cell Death and Potentiates Cancer Immunotherapy. ACS Nano 13 (10), 11967–11980. doi:10.1021/acsnano.9b06040
Meng, Z., Zhou, X., Xu, J., Han, X., Dong, Z., Wang, H., et al. (2019). Light-Triggered In Situ Gelation to Enable Robust Photodynamic-Immunotherapy by Repeated Stimulations. Adv. Mater. 31 (24), e1900927. doi:10.1002/adma.201900927
Ng, C. W., Li, J., and Pu, K. (2018). Recent Progresses in Phototherapy-Synergized Cancer Immunotherapy. Adv. Funct. Mater. 28 (46), 1803272–1803280. doi:10.1002/adfm.201870327
Shen, J., Chen, J., Ke, Z., Zou, D., Sun, L., and Zou, J. (2019). Heavy Atom-free Semiconducting Polymer with High Singlet Oxygen Quantum Yield for Prostate Cancer Synergistic Phototherapy. Mater. Chem. Front. 3 (6), 1123–1127. doi:10.1039/c9qm00158a
Siegel, R. L., Miller, K. D., Fuchs, H. E., and Jemal, A. (2021). Cancer Statistics, 2021. CA A. Cancer J. Clin. 71 (1), 7–33. doi:10.3322/caac.21654
Tang, W., Yang, Z., Wang, S., Wang, Z., Song, J., Yu, G., et al. (2018). Organic Semiconducting Photoacoustic Nanodroplets for Laser-Activatable Ultrasound Imaging and Combinational Cancer Therapy. ACS Nano 12 (3), 2610–2622. doi:10.1021/acsnano.7b08628
Turan, I. S., Yildiz, D., Turksoy, A., Gunaydin, G., and Akkaya, E. U. (2016). A Bifunctional Photosensitizer for Enhanced Fractional Photodynamic Therapy: Singlet Oxygen Generation in the Presence and Absence of Light. Angew. Chem. Int. Ed. 55 (8), 2875–2878. doi:10.1002/anie.201511345
Wang, M., Song, J., Zhou, F., Hoover, A. R., Murray, C., Zhou, B., et al. (2019). NIR‐Triggered Phototherapy and Immunotherapy via an Antigen‐Capturing Nanoplatform for Metastatic Cancer Treatment. Adv. Sci. 6 (10), 1802157. doi:10.1002/advs.201802157
Wang, Z., Zhan, M., Li, W., Chu, C., Xing, D., Lu, S., et al. (2021). Photoacoustic Cavitation‐Ignited Reactive Oxygen Species to Amplify Peroxynitrite Burst by Photosensitization‐Free Polymeric Nanocapsules. Angew. Chem. Int. Ed. 60, 4720–4731. doi:10.1002/anie.202013301
Wang, Z., and Zhao, J. (2017). Bodipy-Anthracene Dyads as Triplet Photosensitizers: Effect of Chromophore Orientation on Triplet-State Formation Efficiency and Application in Triplet-Triplet Annihilation Upconversion. Org. Lett. 19 (17), 4492–4495. doi:10.1021/acs.orglett.7b02047
Wu, C., Guan, X., Xu, J., Zhang, Y., Liu, Q., Tian, Y., et al. (2019). Highly Efficient Cascading Synergy of Cancer Photo-Immunotherapy Enabled by Engineered Graphene Quantum dots/photosensitizer/CpG Oligonucleotides Hybrid Nanotheranostics. Biomaterials 205, 106–119. doi:10.1016/j.biomaterials.2019.03.020
Xiao, F., Cao, B., Wen, L., Su, Y., Zhan, M., Lu, L., et al. (2020). Photosensitizer Conjugate-Functionalized Poly(hexamethylene Guanidine) for Potentiated Broad-Spectrum Bacterial Inhibition and Enhanced Biocompatibility. Chin. Chem. Lett. 31 (9), 2516–2519. doi:10.1016/j.cclet.2020.06.038
Xu, D., Liu, J., Wang, Y., Jian, Y., Wu, W., and Lv, R. (2020). Black Phosphorus Nanosheet with High Thermal Conversion Efficiency for Photodynamic/Photothermal/Immunotherapy. ACS Biomater. Sci. Eng. 6 (9), 4940–4948. doi:10.1021/acsbiomaterials.0c00984
Xu, J., Xu, L., Wang, C., Yang, R., Zhuang, Q., Han, X., et al. (2017). Near-Infrared-Triggered Photodynamic Therapy with Multitasking Upconversion Nanoparticles in Combination with Checkpoint Blockade for Immunotherapy of Colorectal Cancer. ACS Nano 11 (5), 4463–4474. doi:10.1021/acsnano.7b00715
Yang, W., Zhang, F., Deng, H., Lin, L., Wang, S., Kang, F., et al. (2020). Smart Nanovesicle-Mediated Immunogenic Cell Death through Tumor Microenvironment Modulation for Effective Photodynamic Immunotherapy. ACS Nano 14 (1), 620–631. doi:10.1021/acsnano.9b07212
Yao, C., Li, Y., Wang, Z., Song, C., Hu, X., and Liu, S. (2020). Cytosolic NQO1 Enzyme-Activated Near-Infrared Fluorescence Imaging and Photodynamic Therapy with Polymeric Vesicles. ACS Nano 14, 1919–1935. doi:10.1021/acsnano.9b08285
Zhang, D., Zhang, J., Li, Q., Song, A., Li, Z., and Luan, Y. (2019). Cold to Hot: Rational Design of a Minimalist Multifunctional Photo-Immunotherapy Nanoplatform toward Boosting Immunotherapy Capability. ACS Appl. Mater. Inter. 11 (36), 32633–32646. doi:10.1021/acsami.9b09568
Zhang, X., Zhang, A., Feng, J., Yi, J., Peng, L., Chen, J., et al. (2021). A Heavy Atom Free Semiconducting Polymer with High Singlet Oxygen Quantum Yield for Photodynamic and Photothermal Synergistic Therapy. Mater. Des., 197. doi:10.1016/j.matdes.2020.109263
Zhao, H., Chen, H., Guo, Z., Zhang, W., Yu, H., Zhuang, Z., et al. (2020). In Situ photothermal Activation of Necroptosis Potentiates Black Phosphorus-Mediated Cancer Photo-Immunotherapy. Chem. Eng. J., 394. doi:10.1016/j.cej.2020.124314
Zhen, Z., Tang, W., Wang, M., Zhou, S., Wang, H., Wu, Z., et al. (2017). Protein Nanocage Mediated Fibroblast-Activation Protein Targeted Photoimmunotherapy to Enhance Cytotoxic T Cell Infiltration and Tumor Control. Nano. Lett. 17 (2), 862–869. doi:10.1021/acs.nanolett.6b04150
Zheng, Z., Liu, H., Zhai, S., Zhang, H., Shan, G., Kwok, R. T. K., et al. (2020). Highly Efficient Singlet Oxygen Generation, Two-Photon Photodynamic Therapy and Melanoma Ablation by Rationally Designed Mitochondria-specific Near-Infrared AIEgens. Chem. Sci. 11, 2494–2503. doi:10.1039/c9sc06441a
Zhou, Z., Song, J., Nie, L., and Chen, X. (2016). Reactive Oxygen Species Generating Systems Meeting Challenges of Photodynamic Cancer Therapy. Chem. Soc. Rev. 45 (23), 6597–6626. doi:10.1039/c6cs00271d
Zhu, J., Zou, J., Zhang, J., Sun, Y., Dong, X., and Zhang, Q. (2019). An Anthracene Functionalized BODIPY Derivative with Singlet Oxygen Storage Ability for Photothermal and Continuous Photodynamic Synergistic Therapy. J. Mater. Chem. B 7 (20), 3303–3309. doi:10.1039/c9tb00180h
Zou, J., Li, L., Yang, Z., and Chen, X. (2021). Phototherapy Meets Immunotherapy: a Win-Win Strategy to Fight against Cancer. Nanophotonics 10 (12), 3229–3245. doi:10.1515/nanoph-2021-0209
Zou, J., Li, L., Zhu, J., Li, X., Yang, Z., Huang, W., et al. (2021). Singlet Oxygen “Afterglow” Therapy with NIR-II Fluorescenct Molecules. Adv. Mater. 33, 2103627. doi:10.1002/adma.202103627
Keywords: heavy-atom–free, DPPA, human kidney cancer, fractionated PDT, synergistic therapy
Citation: Shen J, Pan L, Zhang X, Zou Z, Wei B, Chen Y, Tang X and Zou D (2022) Delivering Singlet Oxygen in Dark Condition With an Anthracene-Functionalized Semiconducting Compound for Enhanced Phototheranostics. Front. Bioeng. Biotechnol. 10:781766. doi: 10.3389/fbioe.2022.781766
Received: 23 September 2021; Accepted: 19 January 2022;
Published: 09 March 2022.
Edited by:
Malcolm Xing, University of Manitoba, CanadaReviewed by:
Qingqing Xiong, Tianjin Medical University Cancer Institute and Hospital, ChinaXianglong Hu, South China Normal University, China
Copyright © 2022 Shen, Pan, Zhang, Zou, Wei, Chen, Tang and Zou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Yongchang Chen, Chenyongchang0327@163.com; Xiaoyan Tang, xytang@cslg.edu.cn; Dengfeng Zou, zdf1226@126.com