Effects of Metallic and Carbon-Based Nanomaterials on Human Pancreatic Cancer Cell Lines AsPC-1 and BxPC-3
Abstract
:1. Introduction
2. Results
2.1. Physicochemical Analysis of Metallic and Carbon-Based Nanostructures
2.2. Membrane Integrity
2.2.1. Metallic Nanoparticles
2.2.2. Carbon-Based Nanoparticles
2.3. Viability Evaluation
2.3.1. Metallic Nanoparticles
2.3.2. Carbon-Based Nanomaterials
2.4. Morphological Evaluation
2.5. Reactive Oxygen Species Detection
2.6. Cytokine Array
3. Discussion
4. Materials and Methods
4.1. Carbon-Based and Metallic Family Nanoparticles
4.2. Physicochemical Analysis of Metallic and Carbon-Based Nanostructures
4.3. Cell Lines
4.4. Membrane Integrity
4.5. Viability Assay
4.6. Morphological Evaluation
4.7. Reactive Oxygen Species Detection
4.8. Cytokine Array
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
Abbreviations
Ag | silver |
Au | gold |
C60(OH)40 | fullerenol |
DMEM | Dulbecco’s Modified Eagle Medium |
DMSO | dimethylsulfoxide |
FBS | foetal bovine serum |
GO | graphene oxide |
HDF | human dermal fibroblasts |
HUVEC | human umbilical vein endothelial cells |
ICAM-1 | intracellular adhesion molecule 1 |
IL-1a | interleukin 1 |
IL-8 | interleukin 8 |
LDH | lactate dehydrogenase |
MIP-1β | macrophage inflammatory protein 1β |
MN | micronuclei |
ND | nano diamond |
NPs | nanoparticles |
Pt | platinum |
ROS | reactive oxygen species |
RPMI 1640 | Roswell Park Memorial Institute 1640 Medium |
TEM | transmission electron microscope |
TIMP-2 | tissue inhibitor of metalloproteinases 2 |
TNF-β | tumour necrosis factor β |
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Sample | ZP by LDE [mV] ± SD | Diameter by DLS [nm] ± SD |
---|---|---|
Ag | −23.2 ± 1.29 | 244.2 ± 53.16 |
Au | −13.3 ± 3.91 | 190.2 ± 54.69 |
Pt | −21.7 ± 2.0 | 80.0 ± 7.36 |
GO | −38.9 ± 0.38 | 419.6 ± 13.31 |
ND | 23.2 ± 0.68 | 157.6 ± 1.12 |
C60(OH)40 | −48.1 ± 6.58 | 185.3 ± 4.45 |
A | B | C | D | E | F | G | H | I | J | K | L | |
---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | Pos | Pos | Neg | Neg | EOTAXIN | EOTAXIN-2 | GCSF | GM-CSF | ICAM-1 | INF-y | I-309 | IL-1α |
2 | Pos | Pos | Neg | Neg | EOTAXIN | EOTAXIN-2 | GCSF | GM-CSF | ICAM-1 | INF-y | I-309 | IL-1α |
3 | IL-1b | IL-2 | IL-3 | IL-4 | IL-6 | IL-6sR | IL-7 | IL-8 | IL-10 | IL-11 | IL-12 p40 | IL-12 p70 |
4 | IL-1b | IL-2 | IL-3 | IL-4 | IL-6 | IL-6sR | IL-7 | IL-8 | 1L-10 | IL-11 | IL-12 p40 | IL-12 p70 |
5 | IL-13 | IL-15 | IL-16 | IL-17 | IP-10 | MCP-1 | MCP-2 | M-CSF | MIG | MIP-1α | MIP-1β | MIP-1δ |
6 | IL-13 | IL-15 | IL-16 | IL-17 | IP-10 | MCP-1 | MCP-2 | M-CSF | MIG | MIP-1α | MIP-1β | MIP-1δ |
7 | RANTES | TGF-β1 | TNF-α | TNF-β | s TNF RI | s TNF RII | PDGF-BB | TIMP-2 | BLANK | BLANK | Neg | Pos |
8 | RANTES | TGF-β1 | TNF-α | TNF-β | s TNF RI | s TNF RII | PDGF-BB | TIMP-2 | BLANK | BLANK | Neg | Pos |
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Wójcik, B.; Sawosz, E.; Szczepaniak, J.; Strojny, B.; Sosnowska, M.; Daniluk, K.; Zielińska-Górska, M.; Bałaban, J.; Chwalibog, A.; Wierzbicki, M. Effects of Metallic and Carbon-Based Nanomaterials on Human Pancreatic Cancer Cell Lines AsPC-1 and BxPC-3. Int. J. Mol. Sci. 2021, 22, 12100. https://doi.org/10.3390/ijms222212100
Wójcik B, Sawosz E, Szczepaniak J, Strojny B, Sosnowska M, Daniluk K, Zielińska-Górska M, Bałaban J, Chwalibog A, Wierzbicki M. Effects of Metallic and Carbon-Based Nanomaterials on Human Pancreatic Cancer Cell Lines AsPC-1 and BxPC-3. International Journal of Molecular Sciences. 2021; 22(22):12100. https://doi.org/10.3390/ijms222212100
Chicago/Turabian StyleWójcik, Barbara, Ewa Sawosz, Jarosław Szczepaniak, Barbara Strojny, Malwina Sosnowska, Karolina Daniluk, Marlena Zielińska-Górska, Jaśmina Bałaban, André Chwalibog, and Mateusz Wierzbicki. 2021. "Effects of Metallic and Carbon-Based Nanomaterials on Human Pancreatic Cancer Cell Lines AsPC-1 and BxPC-3" International Journal of Molecular Sciences 22, no. 22: 12100. https://doi.org/10.3390/ijms222212100