Urinary Metabolomic Profiling Reveals the Effect of Shenfu Decoction on Chronic Heart Failure in Rats
Abstract
:1. Introduction
2. Results and Discussion
2.1. Hemodynamic Assessment
Time (week) | Group | LVSP (mmHg) | LVEDP (mmHg) | +dp/dtmax (mmHg) | −dp/dtmax (mmHg) |
---|---|---|---|---|---|
8 | Normal Controls | 174.70 ± 2.06 | −16.68 ± 2.42 | 7455.01 ± 643.01 | −4964.79 ± 749.17 |
Sham Surgery | 152.54 ± 27.96 | −18.61 ± 4.85 | 5916.10 ± 779.03 | −4677.67 ± 656.71 | |
CHF group | 120.81 ± 11.40 Δ,* | 2.09 ± 0.10 Δ,* | 2622.39 ± 744.60 Δ,* | −2357.32 ± 482.95 Δ,* | |
11 | Normal Controls | 137.33 ± 13.27 | −5.38 ± 3.01 | 12019.08 ± 732.35 | −9542.12 ± 1101.26 |
Sham Surgery | 128.20 ± 6.98 | −7.73 ± 3.43 | 9312.59 ± 1312.69 | −8106.91 ± 634.04 | |
CHF group | 105.46 ± 4.37 Δ,* | 2.84 ± 0.74 Δ,* | 6694.55 ± 712.63 Δ,* | −6925.12 ± 483.54 Δ,* | |
SFD-treated | 125.28 ± 6.25 # | −2.60 ± 1.64 # | 8453.47 ± 979.84 # | −8565.22 ± 942.11 # |
2.2. Urinary Metabolite Profiling in Rats by GC–MS
2.3. Evaluation of SFD Therapeutic Efficacy
2.4. Discussion
3. Experimental Section
3.1. Ethics Statement
3.2. Chemical Reagents and Materials
3.3. Animal Models
3.4. Extraction of Metabolites from Urine
3.5. GC–TOF–MS Analysis of Urine Extract
3.6. GC–TOF–MS Data Analysis
3.7. Statistical Analysis
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Yang, D.; Wang, X.; Wu, Y.; Lu, B.; Yuan, A.; Leon, C.; Guo, N. Urinary Metabolomic Profiling Reveals the Effect of Shenfu Decoction on Chronic Heart Failure in Rats. Molecules 2015, 20, 11915-11929. https://doi.org/10.3390/molecules200711915
Yang D, Wang X, Wu Y, Lu B, Yuan A, Leon C, Guo N. Urinary Metabolomic Profiling Reveals the Effect of Shenfu Decoction on Chronic Heart Failure in Rats. Molecules. 2015; 20(7):11915-11929. https://doi.org/10.3390/molecules200711915
Chicago/Turabian StyleYang, Dawei, Xiaoxing Wang, Yaping Wu, Bo Lu, Aifeng Yuan, Carlos Leon, and Na Guo. 2015. "Urinary Metabolomic Profiling Reveals the Effect of Shenfu Decoction on Chronic Heart Failure in Rats" Molecules 20, no. 7: 11915-11929. https://doi.org/10.3390/molecules200711915