Insertion of Calcium-Permeable AMPA Receptors during Epileptiform Activity In Vitro Modulates Excitability of Principal Neurons in the Rat Entorhinal Cortex
Abstract
:1. Introduction
2. Results
2.1. Epileptiform Activity Increases the Membrane Input Conductance of the Entorhinal Neurons
2.2. The Effect of a CP-AMPAR Blockade on the Generation of SSDs
2.3. The Input Conductance Decrease following IEM-1460 Application Results in an Increased Probability of Discharges
2.4. Simulations of the Epileptiform Activity
3. Discussion
3.1. Membrane Conductance as a Factor of Seizure Generation
3.2. The Physiological Role of Abnormal Expression of CP-AMPARs during Seizures
4. Materials and Methods
4.1. Animals
4.2. Brain Slice Preparation
4.3. In Vitro Model of Epileptiform Activity
4.4. The Whole-Cell Patch-Clamp Recordings
4.5. Statistics
4.6. The Mathematical Model of Epileptiform Activity
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Appendix A
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Amakhin, D.V.; Soboleva, E.B.; Chizhov, A.V.; Zaitsev, A.V. Insertion of Calcium-Permeable AMPA Receptors during Epileptiform Activity In Vitro Modulates Excitability of Principal Neurons in the Rat Entorhinal Cortex. Int. J. Mol. Sci. 2021, 22, 12174. https://doi.org/10.3390/ijms222212174
Amakhin DV, Soboleva EB, Chizhov AV, Zaitsev AV. Insertion of Calcium-Permeable AMPA Receptors during Epileptiform Activity In Vitro Modulates Excitability of Principal Neurons in the Rat Entorhinal Cortex. International Journal of Molecular Sciences. 2021; 22(22):12174. https://doi.org/10.3390/ijms222212174
Chicago/Turabian StyleAmakhin, Dmitry V., Elena B. Soboleva, Anton V. Chizhov, and Aleksey V. Zaitsev. 2021. "Insertion of Calcium-Permeable AMPA Receptors during Epileptiform Activity In Vitro Modulates Excitability of Principal Neurons in the Rat Entorhinal Cortex" International Journal of Molecular Sciences 22, no. 22: 12174. https://doi.org/10.3390/ijms222212174