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Review

A Literature Review of Host Feeding Patterns of Invasive Aedes Mosquitoes in Europe

1
Estación Biológica de Doñana, Departamento de Ecología de Humedales, Av. Américo Vespucio 26, 41092 Sevilla, Spain
2
Departamento de Parasitología, Facultad de Farmacia, Campus Universitario de Cartuja, Universidad de Granada, 18071 Granada, Spain
3
Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública (CIBERESP), 28029 Madrid, Spain
*
Authors to whom correspondence should be addressed.
Insects 2020, 11(12), 848; https://doi.org/10.3390/insects11120848
Submission received: 22 October 2020 / Revised: 24 November 2020 / Accepted: 25 November 2020 / Published: 29 November 2020
(This article belongs to the Special Issue Vector-Borne Diseases in a Changing World)

Abstract

:

Simple Summary

Invasive mosquito species alter the local epidemiology of many pathogens in the invaded areas, including locality circulating pathogens and imported ones. Four invasive species of the genus Aedes are established in Europe, potentially affecting the transmission of vector-borne diseases in the area. These species include Aedes aegypti, Aedes albopictus, Aedes japonicus and Aedes koreicus. Here, we extensively review the blood feeding patterns of these invasive Aedes mosquitoes which constitute a key parameter affecting the contact rates between infected and susceptible hosts, thus playing a central role in epidemiology of mosquito-borne pathogens. Our results show that these mosquito species feed on different vertebrate groups, especially on mammals. Humans are common hosts of these species, representing 36% and 93% of the blood meals identified for Aedes japonicus and Aedes aegypti, respectively. Birds and, even, ectotherms have been recorded as potential hosts of these Aedes invasive mosquitoes. Given their competence for the transmission of emerging arboviruses such as dengue or Chikungunya viruses and their rates of feeding in humans, Aedes invasive species may have an important impact in the transmission of these pathogens in urban and periurban areas. Finally, we identify the knowledge gaps on the blood feeding patterns of these species and propose directions for future research.

Abstract

Aedes invasive mosquitoes (AIMs) play a key role as vectors of several pathogens of public health relevance. Four species have been established in Europe, including Aedes aegypti, Aedes albopictus, Aedes japonicus and Aedes koreicus. In addition, Aedes atropalpus has been repeatedly recorded although it has not yet been established. In spite of their importance in the transmission of endemic (e.g., heartworms) and imported pathogens (e.g., dengue virus), basic information of parameters affecting their vectorial capacity is poorly investigated. The aim of this study is to review the blood feeding patterns of these invasive mosquito species in Europe, summarizing available information from their native and introduced distribution ranges. The feeding patterns of mosquitoes constitute a key parameter affecting the contact rates between infected and susceptible hosts, thus playing a central role in the epidemiology of mosquito-borne pathogens. Our results highlight that these mosquito species feed on the blood of different vertebrate groups from ectotherms to birds and mammals. However, humans represent the most important source of blood for these species, accounting for 36% and 93% of hosts identified for Ae. japonicus and Ae. aegypti, respectively. In spite of that, limited information has been obtained for some particular species, such as Ae. koreicus, or it is restricted to a few particular areas. Given the high vector competence of the four AIM species for the transmission of different emerging arboviruses such as dengue, Chikungunya, Zika or Yellow fever viruses and their high feeding rates on humans, these AIM species may have an important impact on the vectorial capacity for such pathogens on urban and periurban areas. Finally, we propose directions for future research lines based on identified knowledge gaps.

1. Introduction

Mosquitoes (family Culicidae) are almost ubiquitous, being absent only from some remote areas such as Antarctica. Mosquitoes are a nuisance for humans because of their bites but they also transmit many pathogens to humans and other animals [1,2]. Among vector-borne diseases, mosquito-borne pathogens are particularly relevant causing malaria, dengue fever, Yellow fever, Japanese encephalitis and lymphatic filariasis, among many other diseases [3,4]. For example, malaria alone is responsible for significant rates of morbidity with approximately 405,000 fatalities annually [5]. Although mosquitoes are particularly abundant in the humid tropics and subtropics, they also represent a public health concern in temperate areas [6,7]. Nowadays, the public and scientific concern on mosquito-borne diseases is increasing as new diseases emerge and others resurge or expand to new geographic areas [8]. This expansion of vector borne diseases is often fuelled by processes of invasion by mosquito species with the capacity to transmit pathogens with large relevance for public health and well adapted to proliferate in urban environments [9,10,11]. For example, the Asian tiger mosquito Aedes albopictus is a well-known vector of pathogens including dengue, Zika and Chikungunya viruses [12,13] and has been involved in dengue outbreaks in France in 2010, Spain in 2018 and in Italy in 2020 [14,15,16], Chikungunya outbreaks in Italy [17] and the local transmission of Zika virus in France [18]. Aedes albopictus has also been involved in the transmission of autochthonous locally circulating pathogens such as the nematode Dirofilaria immitis in Italy [19]. In addition, mosquito invasions and the pathogens they can transmit may also have important negative impact on wildlife populations. This is the case of the introduction of Culex pipiens in the Hawaii archipelago that has allowed the local transmission of the avian malaria parasite Plasmodium relictum among immunologically naïve endemic avian species [20]. This parasite significantly contributed to the decline of native bird populations [21].
The aim of this study is to review the published information on the blood feeding patterns of invasive mosquitoes in Europe. In particular, we focus on species of the genus Aedes, which are vectors of both introduced and native pathogens of public health relevance [9,10,22]. The blood feeding patterns of mosquitoes is a key component in the estimation of their vectorial capacity as its study allows the identification of the potential vertebrate hosts of mosquitoes, estimate contact rates and represent an essential component in epidemiological studies of mosquito-borne pathogens [23,24]. In fact, human-biting rate is an important parameter for the estimation of the basic reproduction rate (R0) of vector borne pathogens like arboviruses, i.e., arthropod-borne viruses. R0 is defined as the average number of new cases expected from an infected individual placed in a population of susceptible hosts [25]. When referring to mosquito-borne diseases affecting humans, other important variables affecting R0 are vector longevity, pathogen development time and vector competence, i.e., the ability of mosquitoes to get infected following an infected blood meal and being able to transmit the pathogen during subsequent bites [26]. Aedes species are becoming a global concern due to their expansion throughout the globe, in particular in the case of Aedes albopictus and Aedes aegypti, which have already spread through the tropics, eastern Asia, Europe and North America [27].

2. Aedes Invasive Mosquitoes in Europe

In recent decades, there have been continuous introduction events of exotic mosquitoes into Europe, facilitated by the global movements of people and goods [28,29,30]. Nowadays, there are four Aedes invasive mosquito (AIM) species with known established populations in Europe, namely, Ae. albopictus, Ae. aegypti, Aedes japonicus and Aedes koreicus [31,32,33,34] (Figure 1). In addition, Aedes atropalpus has been detected in several occasions in different European countries such as France and the Netherlands although it has not been established yet [35].
Aedes albopictus is native to Southeast Asia but has spread its distribution to areas around the globe in the last 40 years. In the 19th century, Ae. albopictus colonized some islands of the Indian and the Pacific Ocean around its native range with the aid of human activities. This species was first detected in Europe (Albania) in 1979 and during the decade of the 1980s, new populations were established in North and South America and Africa [36,37,38,39]. This rapid spread has been possible due to the international trade, primarily of used tires [40,41] but also in other kind of shipments such as lucky bamboo, Dracaena sanderiana [42,43]. Further spread within countries may be facilitated by passive transport in vehicles [44]. Nowadays, Ae. albopictus is widely spread and established in more than 15 European countries [31] including Spain [45], France [46], Italy [47], Malta [48,49], Greece [50] and Montenegro [51].
Aedes aegypti, the yellow fever mosquito, was thought to be native to Africa, but now there is genetic evidence of its origin from Madagascar [52]. Nowadays, it is one of the most globally widespread mosquito species [9]. Its disseminations likely started in the 16th century, linked to the slave trade between Africa and the Americas [53]. In the early 20th century, this species occurred in European countries of the Mediterranean basin such as Spain, Greece, France, Italy, among others, but was eradicated from the area after 1950s, probably due to the malaria winter control campaigns [54,55]. Nowadays, Ae. aegypti is present in the Madeira islands [56] and in more eastern countries like Georgia, northeastern Turkey [57], and southern Russia [32].
Aedes japonicus was originally distributed in southern China, Korea, Japan, Taiwan and southern Russia [58]. Of the four known subspecies of Ae. japonicus, only Aedes j. japonicus, native to Japan, Korea, and Russian Primorsky Krai region, has become invasive [58]. Aedes japonicus was found established outside its native range for the first time in the United States [59] while the first report in Europe was in France in 2000 [60]. Since then, established populations of this mosquito species have also been detected in Belgium [61], Germany, Switzerland [62], Austria, Slovenia, Croatia [63], the Netherlands [64], Italy, Hungary [33] and Luxembourg [58]. More recently, thanks to a citizen science platform, Ae. japonicus has been also recorded in some regions of North Spain, where it is currently established [65].
Aedes koreicus is native to Asia, being present in South Korea, Japan, China, and eastern Russia [66]. This species was first detected outside its range in Belgium in 2008, where it is currently established [67]. Since then, the species has been recorded in Italy [68], Slovenia [69], Germany [70], European Russia [71], Hungary [72], and Switzerland [73]. The species is nowadays established in all the mentioned countries except Slovenia and Switzerland [34].
Finally, the American rock pool mosquito Ae. atropalpus, native from eastern North America, expanded its distribution in America due to the utilization of tires as breeding sites and the commerce of used tires through the continent [9]. In the 1990s, this species was reported in northern Italy in a used tires wholesale that imported tires from North America [28]. However, the rapid implementation of control treatments avoided the establishment of the population [47]. Aedes atropalpus was subsequently reported in 2003 and 2005 in France, and in the Netherlands in 2009, but in all the cases the populations were eradicated [9,74]. Nowadays, there are not known established populations of Ae. atropalpus in Europe, although it is introduced in the south of the Netherlands [35].

3. Methods Used for the Identification of Vertebrate Hosts of Invasive Aedes Mosquitoes

Mosquito species differ in their feeding preferences, which determine their contact rates with both pathogens and vertebrate hosts [75,76]. Due to the differential vector competence of mosquitoes and host susceptibility for pathogens, knowledge on the feeding patterns of mosquitoes provides valuable information to identify the key vectors of pathogens, its main reservoirs and also to assess the risk of transmission to humans and other target species. In the case of AIMs, studying their blood-feeding preferences may help to understand how they could affect the local transmission of circulating pathogens and how the risk of local transmission of native and imported pathogens is changed by invasive mosquito presence.
Mosquitoes with a recent blood meal in their abdomen could be used to trace their vertebrate host species. Different approaches have been used for the identification of mosquito’s blood meal sources, including precipitin test [77,78], gel diffusion [79,80] or enzyme-linked immunosorbent assay (ELISA) [81,82], and molecular techniques [83,84]. More recently, the matrix-assisted laser desorption ionization-time off light mass spectrometry (MALDI-TOF MS) has also been applied to mosquito’s blood meal identification. This is a proteomic technique based on the profiling of the blood meal proteins and the identification of the host by comparison with a reference database. This technique has already been used successfully for host identification in Ae. albopictus raised in laboratory, both for single and mixed blood meals [85,86], although there is still scarce evidence of its effectiveness identifying blood meals from field collected Aedes mosquitoes [87] given the large diversity of vertebrates present in wild communities. Another technique that has been recently used for blood-meal identification of mosquitoes is mid-infrared spectroscopy [88]. Although, to our knowledge, it has not been already used with invasive Aedes species, this methodology may represent an additional, less expensive and quicker alternative to other widely used methods [88]. Further information on the blood feeding patterns of these mosquito species could be obtained by exposing different hosts (e.g., humans or other animals) to mosquito attacks [89,90]. The novel mosquito electrocuting trap may allow researchers to identify the risk of exposure of humans to mosquito bites, including invasive Aedes species [91].
All of these different approaches for the identification of vertebrate hosts of mosquitoes have pros and cons that should be evaluated in terms of accessibility to specialized laboratory equipment, time, conservation of the samples, precision in host species identification and economic costs of analyses. For example, serological methods, such as ELISA or precipitin test, consist of the identification of hosts by exposing the blood to immunoglobulin G (IgG) conjugated against potential host species. Therefore, this technique is limited by the availability of antisera against some target species and the cross-reactivity between serum proteins in the case of closely related species limiting the range of hosts that can be identified [23,92]. Both of these techniques have been broadly used to identify blood meals of Aedes species [93,94,95]. To overcome the limitations regarding the availability of antisera and cross-reactivity, some of these studies used a combination of different techniques. For example, Savage et al. [96] used both precipitin test and ELISA to check for possible false positives, while Richards et al. [97] and Apperson et al. [81] employed PCR to conduct the specific identification of blood meals previously identified as avian-derived using ELISA. A similar procedure was used by Jansen et al. [92] with subsamples of blood meals that tested negative using an ELISA identification approach. On the other hand, the use of MALDI-TOF MS is still limited by the low number of species included in the database [85], although available information is progressively growing [98]. Molecular methods can be used to overcome this limitation, allowing increased specificity in the host identification. They consist of the amplification of sequences from different genes using either specific or universal primers. These techniques include DNA sequencing, use of group-specific primers, heteroduplex analysis, PCR-restriction fragment length polymorphism (PCR-RFLM), real-time PCR, reverse line-blot hybridation and DNA profiling [24]. DNA sequencing is the simplest and most specific method and is ideal for insects that feed on a wide range of vertebrate hosts or whose range of hosts is unknown. Once the sequence is obtained, matches can be found in available databases such as GenBank or the Barcode of Life Data System [24,99]. However, these approaches may be limited by factors including the gradual digestion of the blood meal that reduce the success of host identification [100,101] and the occurrence of partial blood meals that may not provide enough starting material [24]. In addition, DNA sequencing does also have constraints because it is time-consuming and relatively expensive, even more in the case of mixed-host blood meals. In addition, different sets of primers may match with sequences of the Ae. albopictus cytochrome (cyt) b gene, consequently amplifying the DNA of the mosquito (one unspecific extra locus) and not of the vertebrate hosts [102,103].
There are different genes that can be used as diagnostic markers for molecular blood meal identification, such as mitochondrial genes, ribosomal RNA genes, nuclear genes and repetitive DNA sequences, including micro and minisatellites [24]. Mitochondrial genes, especially cyt b and c oxidase 1 (COI) genes, are, by far, the most broadly used genes for the molecular identification of mosquito blood meals sources. Within their advantages are the high number of copies and the high variability they present even between closely related species. However, nuclear genes have also been used successfully for identification of blood meals from vectors, but they present restrictions such as the low variability of the sequences in closely related species and that a low number of vertebrates have been characterized for these genes [104]. In addition, mammal blood cells are enucleated, limiting the use of these genes and highlighting the value of working with mitochondrial sequences [24].

4. Blood Feeding Patterns of Invasive Aedes Mosquitoes

We developed an extensive literature review on the blood feeding patterns of Ae. aegypti, Ae. albopictus, Ae. japonicus and Ae. koreicus—the four AIMs currently established in Europe. Studies considered here include those covering both their native and introduced distribution ranges. We used Google Scholar as the main search engine to find articles identifying vertebrate hosts of mosquitoes. We made searches with keywords including “Blood meal AND the scientific name of the mosquito species (e.g., Aedes albopictus)” (February, 2020) and “Feeding pattern AND the scientific name of the mosquito species” (March, 2020). This procedure was conducted for Ae. albopictus, Ae. aegypti, Ae. japonicus and Ae. koreicus. Additional references were obtained from the citations in these studies while other references were facilitated by colleagues. Overall, 276 studies were obtained at a first stage. We only selected studies based on the identification of blood meals of mosquitoes. From them, only those including information about the blood-feeding patterns of wild-caught invasive Aedes mosquitoes were selected. Studies that did not include data about the species focus of this study or that studied mosquitoes raised or fed in the laboratory were not used. Review articles were not considered here. As a result, we found 46 studies on the blood feeding patterns for four of the mosquito species that are included in this study. An Excel table was created including the information of interest, such as species studied, country where the study was developed, the habitat characteristics of the area of capture (i.e., urban, periurban or rural areas), total number of mosquitoes analyzed, methodology of blood meal identification, and the proportion of feedings obtained from different vertebrate hosts.
Aedes albopictus was the species most intensively studied with 31 articles focused on this species, followed by Ae. aegypti, Ae. japonicus and Ae. koreicus, which were included in 14, 8 and 2 studies, respectively. Overall, the dataset included in this study corresponded to 11,618 engorged mosquitoes. Of them, 6448 corresponded to Ae. aegyti, 4893 to Ae. albopictus and 227 to Ae. japonicus. Information of the vertebrate hosts from only 50 blood meals corresponded to Ae. koreicus. Most (80.4%; n = 37) of these studies were conducted in the invaded distribution range of these mosquito species, with only 11 of them being developed in Europe. Only five studies included data from both invaded and native distribution areas. No studies focusing on the identification of vertebrate hosts of Ae. atropalpus were found. Within these studies, 14 used serological methods, namely, ELISA, precipitin test or immunodiffusion technique, for the blood meal identification, while 27 used molecular methods and five combined both of them (four used ELISA and PCR and one used precipitin test and PCR).
According to the published information, 37 species were identified as vertebrate hosts of at least one of the AIM species studied, including 26 mammals and 11 birds. Ten other vertebrate groups were also identified as hosts of these AIMs, although the accuracy of identification reached levels above host genus. The broader host range was recorded for Ae. albopictus including 20 mammal and 5 bird species (Table 1), and Ae. albopictus was the only one that was documented to feed on ectotherms, including reptiles, amphibians and fish (Figure 2a). Aedes aegypti and Ae. japonicus fed on 5 mammal and 4 bird species and 15 mammal and 5 bird species, respectively. Only three species of mammals were identified as hosts of Ae. koreicus, with no records of birds. Results of the percentage of vertebrate host groups of the four AIM species are shown in Figure 2, including those studies developed in Europe (Figure 2b). Despite the ability of most of these species to feed on blood from different vertebrate groups, the vast majority of blood meals derived from mammals, representing over the 90% of the single blood meals identified. Interestingly, the anthropophilic behavior of these species is supported by the fact that humans represent 36–93% of the total single blood meals. Avian sources represented only 0–6% of the single blood meals.
The occurrence of mixed blood meals, those containing blood from two or more vertebrate host species, was highly variable between studies. The proportion of these mixed meals ranged from <1% to 78% of the total feedings for Ae. aegypti, and from 3% to 100% for Ae. albopictus, although this extremely high value was reported by a study where only seven individuals were analyzed [82]. For the case of Ae. japonicus, authors recorded a percentage of 8% and 50% of mixed blood meals in the only two studies recording its occurrence, although the highest value was obtained in a study including only two mosquitoes [105]. To our knowledge, the occurrence of mixed blood meals has not been reported for Ae. koreicus, likely due to the low number of studies and mosquitoes analyzed for this species.

5. Concluding Remarks and Future Prospects

Some of the AIMs studied, particularly Ae. albopictus and Ae. japonicus, have a wide range of hosts, feeding on a broad diversity of vertebrates. All the AIMs had a marked feeding preference for mammals, especially to feed on humans. In particular, human blood represented over 90% of the blood meals identified for Ae. aegypti and Ae. koreicus (Figure 2). This large percentage of human-derived blood meals could be explained, at least in part, due to the nature of the collection sites. Although sampling sites include urban, periurban and rural areas [93,129,130], where the availability of humans may differ, most studies have been conducted in urban environments, especially when studying Ae. albopictus and Ae. aegypti. Aedes aegypti has a highly anthropophilic behavior, entering houses to feed on human blood and breeding in man-made containers in most of its populations [131]. Birds, and other vertebrates, have been also recorded as potential hosts for these AIMs, although they represent a low percentage of the mosquito blood meals studied. Aedes albopictus was the species with the highest percentage of avian blood meals, and the only one found to feed on ectotherms. When focusing on studies developed in Europe, a slight change can be appreciated in the proportions of the blood meal sources in Ae. albopictus and Ae. japonicus. Aedes albopictus shows an increase in the proportion of feedings obtained from humans, which is not surprising given the fact that all six studies had collection sites in urban environments, and only two combined urban and periurban environments. Aedes japonicus, on the contrary, presents a decrease in the anthropophilic pattern. Again, this can be caused by the nature of the collection sites, since the mosquitoes were captured in a zoo and in two resellers of used tires. Nevertheless, the data collected in Europe are scarce, including the study of a relatively low sample size of mosquitoes collected in a handful of countries. Thus, it would be desirable to develop additional studies in Europe to understand seasonal and spatial variation in AIM blood feeding behavior and the impact of urbanization on mosquito feeding patterns and its potential consequences for pathogen transmission in villages and cities. Based on these results and information of their vector competence, AIMs may play an important role in the transmission of pathogens circulating between mammals, especially humans. However, although avian pathogens such as avian malaria have been detected in Ae. albopictus, its relevance may be considered low compared to other species such as Culex pipiens [84,103,106]. This may be also the case for the transmission of zoonotic pathogens of avian origin such as West Nile virus, in spite that this species has been reported as a potential bridge vector between birds and humans in invaded ecosystems [132].
Interestingly, studies on the feeding preferences of mosquitoes are still scarce as most of the published information did not consider the abundance of the potential vertebrate hosts in the studied localities. This could lead, for example, to an overestimation of the anthropophilic behavior of AIMs in studies developed exclusively in urbanized environments where humans may represent the most common available hosts. In fact, a recent study identified urbanization as a key component explaining the variation of Ae. aegypti preference for human odors [133]. This anthropophilic behavior showed in urbanized areas [103,107] could facilitate the transmission of mosquito-borne pathogens and facilitate local transmission arising from virus imported by infected travelers. Thus, future studies on the feeding pattern of AIMs may be carried out combined with censuses of vertebrate hosts in the area.
This review also highlights other limitations of the current knowledge of the feeding patterns of AIMs in Europe due to the extremely low number of mosquitoes analyzed for some species. This is particularly the case for Ae. koreicus, for which records of blood meal hosts have been obtained for only 50 mosquitoes. These numbers are even lower when considering just the blood meals analyzed from European populations of mosquitoes. It is important to highlight that studies focused on AIMs are still scarce in Europe. Specifically, we found only ten studies that analyzed the feeding patterns of AIMs in the area, but they were developed in areas corresponding to only five countries (Spain, Italy, the Netherlands, Switzerland and Madeira (Portugal). This fact may be partially due to the limited distribution range of most of these mosquito species in Europe. However, the blood feeding patterns of Ae. albopictus have only been studied in Spain, Italy and Switzerland [73,84,103,106,112,129], despite being currently established in more than 15 European countries [31]. In addition, previous studies have not analyzed the factors affecting spatial or seasonal variation in blood meal composition or in the incidence of human blood meals in this species. Furthermore, we found no data on the feeding patterns of Ae. atropalpus. This is not surprising in the case of Europe, since the species was eradicated from different countries and nowadays there are not known established populations, although it is present in a small area in the Netherlands [35].
Overall, these findings highlight a major gap of knowledge that should be fulfilled in the future to finally understand how the establishment of Aedes species has changed the patterns of transmission of mosquito-borne pathogens in Europe and the risk of local outbreaks of imported arboviruses and other imported pathogens.
In addition, molecular and serological techniques can be used to identify pathogens present in the abdomen of blood-engorged insects (e.g., xenosurveillance) [134,135]. This procedure allows researchers to obtain information not only about mosquitoes feeding preferences, but also which pathogens are interacting with them and are circulating in the populations.

Supplementary Materials

The following are available online at https://www.mdpi.com/2075-4450/11/12/848/s1, Table S1: Vertebrate hosts of Aedes invasive species identified using different approaches.

Author Contributions

S.C.-C. wrote the first draft of the manuscript with considerable assistance from J.M.-d.l.P. and J.F. All authors contributed to manuscript revision. All authors have read and agreed to the published version of the manuscript.

Funding

This study was funded by the project PGC2018-095704-B-I00 from the Spanish Ministry of Science and Innovation and the European Regional Development Fund. The work of J.M.-d.l.P. and J.F. was conducted within the framework of the AIM-COST Action CA17108.

Acknowledgments

We thank Krisztian Magori and two anonymous reviewers for their constructive comments, which helped us to improve the manuscript.

Conflicts of Interest

The authors declare no competing interests.

References

  1. Service, M. Mosquitoes (Culicidae). In Medical Insects and Arachnids; Lane, R.P., Crosskey, R.W., Eds.; Chapman Hall: London, UK, 1993; pp. 120–122. [Google Scholar]
  2. Becker, N.; Petrić, D.; Zgomba, M.; Boase, C.; Madon, M.; Dahl, C.; Kaiser, A. Mosquitoes and Their Control, 2nd ed.; Springer: Berlin/Heidelberg, Germany, 2010; pp. 27–29. [Google Scholar]
  3. Tolle, M. Mosquito-borne Diseases. Curr. Probl. Pediatr. Adolesc. Health Care 2009, 39, 97–140. [Google Scholar] [CrossRef] [PubMed]
  4. Kampen, H.; Kronefeld, M.; Werner, D. Culicid Mosquitoes as Vectors of Disease Agents in Europe. In Arthropods as Vectors of Emerging Diseases; Mehlhorn, H., Ed.; Springer: Düsseldorf, Germany, 2012; pp. 1–3. [Google Scholar]
  5. World Health Organization. World Malaria Report; World Health Organization: Geneva, Switzerland, 2019; ISBN 978-92-4-156572-1. [Google Scholar]
  6. Huang, Y.; Higgs, S.; Vanlandingham, D. Emergence and re-emergence of mosquito-borne arboviruses. Curr. Opin. Virol. 2019, 34, 104–109. [Google Scholar] [CrossRef] [PubMed]
  7. Franklinos, L.H.V.; Jones, K.E.; Redding, D.W.; Abubakar, I. The effect of global change on mosquito-borne disease. Lancet Infect. Dis. 2019, 19, 302–312. [Google Scholar] [CrossRef]
  8. Brugueras, S.; Fernández-Martínez, B.; Martínez-de la Puente, J.; Figuerola, J.; Montalvo Porro, T.; Rius, C.; Larrauri, A.; Gómez-Barroso, D. Environmental drivers, climate change and emergent diseases transmitted by mosquitoes and their vectors in southern Europe: A systematic review. Environ. Res. 2020, 191, 110038. [Google Scholar] [CrossRef] [PubMed]
  9. Medlock, J.M.; Hansford, K.M.; Versteirt, V.; Cull, B.; Kampen, H.; Fontenille, D.; Hendrickx, G.; Zeller, H.; Van Bortel, W.; Schaffner, F. An entomological review of invasive mosquitoes in Europe. Bull. Entomol. Res. 2015, 105, 637–663. [Google Scholar] [CrossRef]
  10. Gratz, N. Critical review of the vector status of Aedes albopictus. Med. Vet. Entomol. 2004, 18, 215–227. [Google Scholar] [CrossRef]
  11. Souza-Neto, J.; Powell, J.; Bonizzoni, M. Aedes aegypti vector competence studies: A review. Infect. Genet. Evol. 2019, 67, 191–209. [Google Scholar] [CrossRef]
  12. Gutiérrez-López, R.; Bialosuknia, S.; Ciota, A.; Montalvo, T.; Martínez-de la Puente, J.; Gangoso, L.; Figueroa, J.; Kramer, L.D. Vector Competence of Aedes caspius and Ae. albopictus Mosquitoes for Zika Virus, Spain. Emerg. Infect. Dis. 2019, 25, 346–348. [Google Scholar] [CrossRef] [Green Version]
  13. Vega-Rúa, A.; Lourenço-de-Oliveira, R.; Mousson, L.; Vazeille, M.; Fuchs, S.; Yébakima, A.; Gustave, J.; Girod, R.; Dusfour, I.; Leparc-Goffart, I.; et al. Chikungunya Virus Transmission Potential by Local Aedes Mosquitoes in the Americas and Europe. PLoS Negl. Trop. Dis. 2015, 9, e0003780. [Google Scholar] [CrossRef] [Green Version]
  14. La Ruche, G.; Souarès, Y.; Armengaud, A.; Peloux-Petiot, F.; Delaunay, P.; Desprès, P.; Lenglet, A.; Jourdain, F.; Leparc-Goffart, I.; Charlet, F.; et al. First two autochthonous dengue virus infections in metropolitan France, September. Eur. Surveill. 2010, 15, 19676. [Google Scholar]
  15. Monge, S.; García-Ortúzar, V.; López Hernández, B.; Lopaz Pérez, M.; Delacour-Estrella, S.; Sánchez-Seco, M.; Fernández Martínez, B.; García San Miguel, L.; García-Fulgueiras, A.; Sierra Moros, M.J. Characterization of the first autochthonous dengue outbreak in Spain (August–September 2018). Acta Trop. 2020, 205, 105402. [Google Scholar] [CrossRef] [PubMed]
  16. Lazzarini, L.; Barzon, L.; Foglia, F.; Manfrin, V.; Pacenti, M.; Pavan, G.; Rassu, M.; Capelli, G.; Montarsi, F.; Martini, S.; et al. First autochthonous dengue outbreak in Italy, August 2020. Eur. Surveill. 2020, 25, 2001606. [Google Scholar] [CrossRef] [PubMed]
  17. Bonilauri, P.; Bellini, R.; Calzolari, M.; Angelini, R.; Venturi, L.; Fallacara, F.; Cordioli, P.; Angelini, P.; Venturelli, C.; Merialdi, G.; et al. Chikungunya Virus in Aedes albopictus, Italy. Emerg. Infect. Dis. 2008, 14, 852–854. [Google Scholar] [CrossRef]
  18. Giron, S.; Franke, F.; Decoppet, A.; Cadiou, B.; Travaglini, T.; Thirion, L.; Durand, G.; Jeannin, C.; L’Ambert, G.; Grard, G.; et al. Vector-borne transmission of Zika virus in Europe, southern France, August. Eur. Surveill. 2019, 24, 1–4. [Google Scholar] [CrossRef] [Green Version]
  19. Cancrini, G.; Frangipane di Regalbono, A.; Ricci, I.; Tessarin, C.; Gabrielli, S.; Pietrobelli, M. Aedes albopictus is a natural vector of Dirofilaria immitis in Italy. Vet Parasitol. 2003, 118, 195–202. [Google Scholar] [CrossRef] [PubMed]
  20. Atkinson, C.T.; Woods, K.; Dusek, R.J.; Sileo, L.S.; Iko, W.M. Wildlife disease and conservation in Hawaii: Pathogenicity of avian malaria (Plasmodium relictum) in experimentally infected Iiwi (Vestiaria coccinea). Ecol. Wildl. Host-Parasite Interact. 1995, 111, S59–S69. [Google Scholar] [CrossRef] [PubMed]
  21. Warner, R. The Role of Introduced Diseases in the extinction of the endemic Hawaiian avifauna. Condor 1968, 70, 101–120. [Google Scholar] [CrossRef]
  22. Haddad, N.; Mousson, L.; Vazeille, M.; Chamat, S.; Tayeh, J.; Osta, M.; Failloux, A. Aedes albopictus in Lebanon, a potential risk of arboviruses outbreak. BMC Infect. Dis. 2012, 12. [Google Scholar] [CrossRef] [Green Version]
  23. Martínez-de la Puente, J.; Ferraguti, M.; Ruiz, S.; Montalvo, T.; Casimiro Soriguer, R.; Figuerola, J. Tracing pathogen transmission by mosquitoes under a global change perspective: On the role of the identification of mosquito bloodmeals. In Enciclopedy of Life Science (eLS); John Wiley & Sons Ltd.: Chichester, UK, 2018. [Google Scholar]
  24. Kent, R. Molecular methods for arthropod bloodmeal identification and applications to ecological and vector-borne disease studies. Mol. Ecol. Resoures 2009, 2009 9, 4–18. [Google Scholar] [CrossRef]
  25. Heesterbeek, J. A brief history of R0 and a receip for its calculation. Acta Biotheor. 2002, 50, 189–204. [Google Scholar] [CrossRef]
  26. Delatte, H.; Desvars, A.; Bouétard, A.; Bord, S.; Gimonneau, G.; Vourc’h, G.; Fontenille, D. Blood-feeding behavior of Aedes albopictus, a vector of Chikungunya on La Réunion. Vector Borne Zoonotic Dis. 2010, 10, 249–258. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  27. Kraemer, M.U.; Sinka, M.E.; Duda, K.A.; Mylne, A.Q.; Shearer, F.M.; Barker, C.M.; Moore, C.G.; Carvalho, R.G.; Coelho, G.E.; Van Bortel, W.; et al. The global distribution of the arbovirus vectors Aedes aegypti and Ae. albopictus. eLife 2015, 4, e08347. [Google Scholar] [CrossRef] [PubMed]
  28. Romi, R.; Sabatinelli, G.; Savelli, L.; Raris, M.; Zago, M.; Malatesta, R. Identification of a North American mosquito species, Aedes atropalpus (Diptera:Culicidae), in Italy. J. Am. Control Assoc. 1997, 13, 245–246. [Google Scholar]
  29. Lounibos, L. Ivasions by insect vectors of human diseases. Annu. Rev. 2002, 44, 233–266. [Google Scholar]
  30. Ibáñez-Justicia, A.; Smitz, N.; den Hartog, W.; van de Vossenberg, B.; De Wolf, K.; Deblauwe, I.; Van Bortel, W.; Jacobs, F.; Vauz, A.G.C.; Medlock, J.M.; et al. Detection of exotic mosquito species (Diptera: Culicidae) at international airports in Europe. Int. J. Environ. Res. Public Health 2020, 17, 3450. [Google Scholar] [CrossRef]
  31. European Centre for Disease Prevention and Control (ECDC). Aedes albopictus—Current Known Distribution: September 2020. Available online: https://www.ecdc.europa.eu/en/publications-data/aedes-albopictus-current-known-distribution-september-2020 (accessed on 20 November 2020).
  32. European Centre for Disease Prevention and Control (ECDC). Aedes aegypti—Current Known Distribution: September 2020. Available online: https://www.ecdc.europa.eu/en/publications-data/aedes-aegypti-current-known-distribution-september-2020 (accessed on 20 November 2020).
  33. European Centre for Disease Prevention and Control (ECDC). Aedes japonicus—Current Known Distribution: September 2020. Available online: https://www.ecdc.europa.eu/en/publications-data/aedes-japonicus-current-known-distribution-september-2020 (accessed on 20 November 2020).
  34. European Centre for Disease Prevention and Control (ECDC). Aedes koreicus—Current Known Distribution: September 2020. Available online: https://www.ecdc.europa.eu/en/publications-data/aedes-koreicus-current-known-distribution-september-2020 (accessed on 20 November 2020).
  35. European Centre for Disease Prevention and Control (ECDC). Aedes atropalpus—Current Known Distribution: September 2020. Available online: https://www.ecdc.europa.eu/en/publications-data/aedes-atropalpus-current-known-distribution-september-2020 (accessed on 20 November 2020).
  36. Izri, A.; Bitam, I.; Charrel, R. First entomological documentation of Aedes (Stegomyia) albopictus (Skuse, 1894) in Algeria. Clin. Microbiol. Infect. 2011, 17, 1116–1118. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  37. Reiter, P.; Sprenger, D. The used tire trade: A mechanism for the worldwide dispersal of container breeding mosquitoes. J. Am. Mosq. Control Assoc. 1987, 3, 494–501. [Google Scholar]
  38. Forattini, O. Identificação de Aedes (Stegomyia) albopictus (Skuse) no Brasil. Rev. Saúde Pública 1986, 20, 244–245. [Google Scholar] [CrossRef]
  39. Cornel, A.J.; Hunt, R.H. Aedes albopictus in Africa? First records of live specimens in imported tires in Cape Town. J. Am. Mosq. Control Assoc. 1991, 7, 107–108. [Google Scholar]
  40. Reiter, P. Aedes albopictus and the world trade in used tires, 1988–1995: The shape of things to come? J. Am. Mosq. Control Assoc. 1998, 14, 83–94. [Google Scholar]
  41. Grist, N.R. Aedes albopictus: The tyre-travelling tiger. J. Infect. 1993, 27, 1–4. [Google Scholar] [CrossRef]
  42. Scholte, E.J.; Dijkstra, E.; Blok, H.; De Vries, A.; Takken, W.; Hofhuis, A.; Koopmans, M.; De Boer, A.; Reusken, C.B.E.M. Accidental importation of the mosquito Aedes albopictus into the Netherlands: A survey of mosquito distribution and the presence of dengue virus. Med. Vet. Entomol. 2008, 22, 352–358. [Google Scholar] [CrossRef] [PubMed]
  43. Demeulemeester, J.; Deblauwe, I.; De Witte, J.; Jansen, J.; Hendy, A.; Madder, M. First interception of Aedes (Stegomyia) albopictus in Lucky bamboo shipments in Belgium. J. Am. Mosq. Control Assoc. 2014, 32, 14–16. [Google Scholar]
  44. Eritja, R.; Palmer, J.; Roiz, D.; Sanpera-Calbet, I.; Bartumeus, F. Direct Evidence of Adult Aedes albopictus Dispersal by Car. Sci. Rep. 2017, 7, 14399. [Google Scholar] [CrossRef] [Green Version]
  45. Aranda, C.; Eritja, R.; Roiz, D. First record and establishment of the mosquito Aedes albopictus in Spain. Med. Vet. Entomol. 2006, 20, 150–152. [Google Scholar] [CrossRef]
  46. Schaffner, F.; Karch, S. Première observation Aedes albopictus (Skuse, 1894) en France métropolitaine. Comptes Rendus Acad. Sci. III Sci. Vie 2000, 323, 373–375. [Google Scholar] [CrossRef]
  47. Romi, R.; Di Luca, M.; Majori, G. Current status of Aedes albopictus and Aedes atropalpus in Italy. J. Am. Mosq. Control Assoc. 1999, 15, 425–427. [Google Scholar]
  48. Gatt, P.; Deeming, J.; Schaffner, F. First record of Aedes (Stegomyia) albopictus (Skuse) (Diptera: Culicidae) in Malta. Zur. Open Repos. Arch. Univ. Zur. 2009, 27, 56–64. [Google Scholar] [CrossRef]
  49. Gatt, P.; Schaffner, F.; Cassar, L. Aedes (Stegomyia) albopictus (Skuse) (Diptera: Culicidae) in Malta—The first winter. Zur. Open Repos. Arch. Univ. Zur. 2010, 28, 225–229. [Google Scholar] [CrossRef]
  50. Benedict, M.Q.; Levine, R.S.; Hawley, W.A.; Lounibos, L.P. Spread of The Tiger: Global Risk of Invasion by The Mosquito Aedes albopictus. Vector Borne Zoonotic Dis. 2007, 7, 76–85. [Google Scholar] [CrossRef] [Green Version]
  51. Petric, D.; Pajovic, I.; Ignjatovic Cupina, A.; Zgomba, M. Aedes albopictus (Skuse, 1894) new mosquito species (Diptera, Culicidae) in entomofauna of Yugoslavia. In Proceedings of the Symposia of the entomologists of Serbia, Belgrade, Serbia, 26–29 September 2001; pp. 26–29. [Google Scholar]
  52. Soghigian, J.; Gloria-Soria, A.; Robert, V.; Le Goff, G.; Failloux, A.; Powell, J.R. Genetic evidence for the origin of Aedes aegypti, the yellow fever mosquito, in the southwestern Indian Ocean. Mol. Ecol. 2020, 29, 3593–3606. [Google Scholar] [CrossRef]
  53. Powell, J.R. Mosquito-borne human viral diseases: Why Aedes aegypti? Am. J. Trop. Med. Hyg. 2018, 98, 1563–1565. [Google Scholar] [CrossRef] [PubMed]
  54. Kramer, I.M.; Kreß, A.; Klingelhöfer, D.; Scherer, C.; Phuyal, P.; Kuch, U.; Ahrens, B.; Groneberg, D.A.; Dhimal, M.; Müller, R. Does winter cold really limit the dengue vector Aedes aegypti in Europe? Parasites Vectors 2020, 13, 178. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  55. Holstein, M. Dynamics of Aedes aegypti Distribution, density and seasonal prevalence in the Mediterranean area. Bull. World Health Organ. 1967, 36, 541–543. [Google Scholar] [PubMed]
  56. Schaffner, F.; Mathis, A. Dengue and dengue vectors in the WHO European region: Past, present and scenarios for the future. Lancet Infect. Dis. 2014, 14, 1271–1280. [Google Scholar] [CrossRef]
  57. Akiner, M.M.; Demirci, B.; Babuadze, G.; Robert, V.; Schaffner, F. Spread of the invasive mosquitoes Aedes aegypti and Aedes albopictus in the Black Sea region increases risk of Chikungunya, dengue, and Zika outbreaks in Europe. PLoS Negl. Trop. Dis. 2016, 10, e0004664. [Google Scholar] [CrossRef] [Green Version]
  58. Schaffner, F.; Ries, C. First evidence and distribution of the invasive alien mosquito Aedes japonicus (Theobald, 1901) in Luxembourg. Bull. Soc. Nat. luxemb. 2019, 121, 169–183. [Google Scholar]
  59. Peyton, E.L.; Campbell, S.R.; Candeletti, T.M.; Romanowski, M.; Crans, W.J. Aedes (Finlaya) japonicus japonicus (Theobald), a new introduction into the United States. J. Am. Mosq. Control Assoc. 1999, 15, 238–241. [Google Scholar]
  60. Schaffner, F.; Chouin, S.; Guilloteau, J. First record of Orchlerotatus (Finlaya) japonicus japonicus (Theobald, 1901) in metropolitan France. J. Am. Mosq. Control Assoc. 2003, 19, 1–5. [Google Scholar]
  61. Versteirt, V.; Schaffner, F.; Garros, C.; Dekoninck, W.; Coosemans, M.; Van Bortel, W. Introduction and establishment of the exotic mosquito species Aedes japonicus japonicus (Diptera: Culicidae) in Belgium. J. Med. Entomol. 2009, 46, 1464–1467. [Google Scholar] [CrossRef] [Green Version]
  62. Schaffner, F.; Kaufmann, C.; Hegglin, D.; Mathis, A. The invasive mosquito Aedes japonicusin Central Europe. Med. Vet. Entomol. 2009, 23, 448–451. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  63. Kampen, H.; Werner, D. Out of the bush: The Asian bush mosquito Aedes japonicus japonicus (Theobald, 1901) (Diptera, Culicidae) becomes invasive. Parasites Vectors 2014, 7, 59. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  64. Ibáñez-Justicia, A.; Kampen, H.; Braks, M.; Schaffner, F.; Steeghs, M.; Werner, D.; Zielke, D.; den Hartog, W.; Brooks, M.; Dik, M.; et al. First report of established population of Aedes japonicus japonicus (Theobald, 1901) (Diptera, Culicidae) in the Netherlands. J. Eur. Mosq. Control Assoc. 2014, 32, 9–13. [Google Scholar]
  65. Eritja, R.; Ruiz-Arrondo, I.; Delacour-Estrella, S.; Schaffner, F.; Álvarez-Chachero, J.; Bengoa, M.; Puig, M.-Á.; Melero-Alcíbar, R.; Oltra, A.; Bartumeus, F. First detection of Aedes japonicus in Spain: An unexpected finding triggered by citizen science. Parasites Vectors 2019, 12, 53. [Google Scholar] [CrossRef] [PubMed]
  66. Knight, K.L. Contributions to the mosquito fauna of Southeast Asia—IV. Species of subgroup Chrysolineatus of group D, genus Aedes, subgenus Finlaya (Theobald). Contrib. Amer. Entomol. Inst. 1968, 2, 1–45. [Google Scholar]
  67. Versteirt, V.; de Clercq, E.M.; Fonseca, D.M.; Pecor, J.; Schaffner, F.; Coosemans, M.; Bortel, W.V. Bionomics of the established exotic mosquito species Aedes koreicus in Belgium, Europe. J. Med. Entomol. 2012, 49, 1226–1232. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  68. Capelli, G.; Drago, A.; Martini, S.; Montarsi, F.; Soppelsa, M.; Delai, N.; Ravagnan, S.; Mazzon, L.; Schaffner, F.; Mathis, A.; et al. First report in Italy of the exotic mosquito species Aedes (Finlaya) koreicus, a potential vector of arboviruses and filariae. Parasites Vectors 2011, 4, 188. [Google Scholar] [CrossRef] [Green Version]
  69. Kalan, K.; Šušnjar, J.; Ivović, V.; Buzan, E. First record of Aedes koreicus (Diptera, Culicidae) in Slovenia. Parasitol. Res. 2017, 116, 2355–2358. [Google Scholar] [CrossRef]
  70. Werner, D.; Zielke, D.E.; Kampen, H. First record of Aedes koreicus (Diptera: Culicidae) in Germany. Parasitol. Res. 2016, 115, 1331–1334. [Google Scholar] [CrossRef]
  71. Bezzhonova, O.V.; Patraman, I.V.; Ganushkina, L.A.; Vyshemirskiĭ, O.I.; Sergiev, V.P. The first finding of invasive species Aedes (Finlaya) koreicus (Edwards, 1917) in European Russia. Med. Parazitol. 2014, 1, 16–19. [Google Scholar]
  72. Kurucz, K.; Kiss, V.; Zana, B.; Schmieder, V.; Kepner, A.; Jakab, F.; Kemenesi, G. Emergence of Aedes koreicus (Diptera: Culicidae) in an urban area, Hungary, 2016. Parasitol. Res. 2016, 115, 4687–4689. [Google Scholar] [CrossRef] [PubMed]
  73. Suter, T.; Flacio, E.; Fariña, B.F.; Engeler, L.; Tonolla, M.; Müller, P. First report of the invasive mosquito species Aedes koreicus in the Swiss-Italian border region. Parasites Vectors 2015, 8, 402. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  74. Scholte, E.J.; Den Hartog, W.; Braks, M.; Reusken, C.; Dik, M.; Hessels, A. First report of a North American invasive mosquito species Ochlerotatus atropalpus (Coquillett) in The Netherlands, 2009. Eur. Surveill. 2009, 14. [Google Scholar] [CrossRef] [PubMed]
  75. Ferraguti, M.; Martínez-de la Puente, J.; Muñoz, J.; Roiz, D.; Ruiz, S.; Soriguer, R.; Figuerola, J. Avian Plasmodium in Culex and Ochlerotatus mosquitoes from southern Spain: Effects of season and host-feeding source on parasite dynamics. PLoS ONE 2013, 8, e66237. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  76. Kilpatrick, A.M.; Kramer, L.D.; Jones, M.J.; Marra, P.P.; Daszak, P. West Nile Virus epidemics in North America are driven by shifts in mosquito feeding behavior. PLoS Biol. 2006, 4, e82. [Google Scholar] [CrossRef] [PubMed]
  77. Weitz, B. Identification of blood meals of blood-sucking arthropods. Bull. World Health Org. 1956, 15, 473–490. [Google Scholar]
  78. Lorosa, E.S.; Faria, M.S.; De Oliveira, L.C.M.; Alencar, J.; Marcondes, C.B. Blood meal identification of selected mosquitoes in Rio De Janeiro, Brazil. J. Am. Mosq. Control Assoc. 2010, 26, 18–23. [Google Scholar] [CrossRef]
  79. Crans, W. An agar gel difussion method for the identification of mosquito blood-meals. J. Ser. New Jersey Agric. Exp. Stn. 1969, 29, 563–566. [Google Scholar]
  80. Srinivasan, R.; Panicker, K. Identification of bloodmeals of phlebotomine sandflies using the agarose gel diffusion method. Southeast Asian J. Trop. Med. Public Health 1992, 23, 486–488. [Google Scholar]
  81. Apperson, C.S.; Hassan, H.K.; Harrison, B.A.; Savage, H.M.; Aspen, S.E.; Farajollahi, A.; Crans, W.; Daniels, T.J.; Falco, R.C.; Benedict, M.; et al. Host feeding patterns of established and potential mosquito vectors of West Nile Virus in the eastern United States. Vector Borne Zoonotic Dis. 2004, 4, 71–82. [Google Scholar] [CrossRef]
  82. Khaklang, S.; Kittayapong, P. Species composition and blood meal analysis of mosquitoes collected from a tourist island, Koh Chang, Thailand. J. Vector Ecol. 2014, 39, 448–452. [Google Scholar] [CrossRef] [PubMed]
  83. Chang, M.-C.; Teng, H.-J.; Chen, C.-F.; Chen, Y.-C.; Jeng, C.-R. The resting sites and blood-meal sources of Anopheles minimus in Taiwan. Malar. J. 2008, 7, 105. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  84. Martínez-de la Puente, J.; Muñoz, J.; Capelli, G.; Montarsi, F.; Soriguer, R.; Arnoldi, D.; Rizzoli, A.; Figuerola, J. Avian malaria parasites in the last supper: Identifying encounters between parasites and the invasive Asian mosquito tiger and native mosquito species in Italy. Malar. J. 2015, 14, 32. [Google Scholar] [CrossRef] [Green Version]
  85. Niare, S.; Berenger, J.-M.; Dieme, C.; Doumbo, O.; Raoult, D.; Parola, P.; Almeras, L. Identification of blood meal sources in the main African malaria mosquito vector by MALDI-TOF MS. Malar. J. 2016, 15, 87. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  86. Tandina, F.; Niare, S.; Almeras, L.; Davoust, B.; Doumbo, O.K.; Raoult, D.; Parola, P.; Laroche, M. Identification of mixed and successive blood meals of mosquitoes using MALDI-TOF MS protein profiling. Parasitology 2020, 147, 329–339. [Google Scholar] [CrossRef] [PubMed]
  87. Tandina, F.; Niaré, S.; Laroche, M.; Koné, A.K.; Diarra, A.Z.; Ongoiba, A.; Berenger, J.M.; Doumbo, O.K.; Raoult, D.; Parola, P. Using MALDI-TOF MS to identify mosquitoes collected in Mali and their blood meals. Parasitology 2018, 145, 1170–1182. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  88. Mwanga, E.P.; Mapua, S.A.; Siria, D.J.; Ngowo, H.S.; Nangacha, F.; Mgando, J.; Baldini, F.; González Jiménez, M.; Ferguson, H.M.; Wynne, K.; et al. Using mid-infrared spectroscopy and supervised machine-learning to identify vertebrate blood meals in the malaria vector, Anopheles arabiensis. Malar. J. 2019, 18, 187. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  89. Sallam, M.F.; Pereira, R.M.; Batich, C.; Koehler, P. Factors Affecting Short-Range Host-Seeking for the Yellow Fever Mosquito (Diptera: Culicidae). J. Med. Entomol. 2019, 56, 609–616. [Google Scholar] [CrossRef]
  90. Beier, J.C. Vector incrimination and entomological inoculation rates. In Malaria Methods and Protocols; Methods in Molecular Medicine™; Doolan, D.L., Ed.; Humana Press Inc.: Totowa, NJ, USA, 2002; Volume 72, pp. 3–11. [Google Scholar]
  91. Ortega-López, L.D.; Pondeville, E.; Kohl, A.; León, R.; Betancourth, M.P.; Almire, F.; Torres-Valencia, S.; Saldarriaga, S.; Mirzai, N.; Ferguson, H.M. The mosquito electrocuting trap as an exposure-free method for measuring human-biting rates by Aedes mosquito vectors. Parasites Vectors 2020, 13, 31. [Google Scholar] [CrossRef]
  92. Jansen, C.; Zborowski, P.; Graham, G.; Webb, C.; Russell, R.; Craig, S.; Zborowski, P.; Ritchie, S.A.; Russell, R.; van den Hurk, A.F. Blood sources of mosquitoes collected from urban and peri-urban environments in eastern Australia with species-specific molecular analysis of avian blood meals. Am. J. Trop. Med. Hyg. 2009, 81, 849–857. [Google Scholar] [CrossRef] [Green Version]
  93. Gomes, A.; Silva, N.; Marques, G.; Brito, M. Host-feeding patterns of potential human disease vectors in the Paraíba Valley Region, State of São Paulo, Brazil. J. Vector Ecol. 2003, 28, 74–78. [Google Scholar] [PubMed]
  94. Tandom, N.; Ray, S. Host feeding pattern of Aedes aegypti and Aedes albopictus in Kolkata, India. Dengue Bull. 2000, 24, 117–120. [Google Scholar]
  95. Ponlawat, A.; Harrington, L.C. Blood feeding patterns of Aedes aegypti and Aedes albopictus in Thailand. J. Med. Entomol. 2005, 42, 844–849. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  96. Savage, H.M.; Niebylski, M.; Smith, G.; Mitchell, C.; Craig, J.R.G. Host-feeding patterns of Aedes albopictus (Diptera: Culicidae) at a temperate North American site. J. Med. Entomol. 1993, 30, 27–34. [Google Scholar] [CrossRef]
  97. Richards, S.L.; Ponnusamy, L.; Unnasch, T.R.; Hassan, H.K.; Apperson, C.S. Host-feeding patterns of Aedes albopictus (Diptera: Culicidae) in relation to availability of human and domestic animals in suburban landscapes of central North Carolina. J. Med. Entomol. 2006, 43, 543–551. [Google Scholar] [CrossRef]
  98. Niare, S.; Tandina, F.; Davoust, B.; Doumbo, O.; Raoult, D.; Parola, P.; Almeras, L. Accurate identification of Anopheles gambiae Giles trophic preferences by MALDI-TOF MS. Infect. Genet. Evol. 2018, 63, 410–419. [Google Scholar] [CrossRef]
  99. Alcaide, M.; Rico, C.; Ruiz, S.; Soriguer, R.; Muñoz, J.; Figuerola, J. Disentangling vector-borne transmission networks: A universal DNA barcoding method to identify vertebrate hosts from arthropod bloodmeals. PLoS ONE 2009, 4, e7092. [Google Scholar] [CrossRef] [Green Version]
  100. Srisawat, R.; Sungvornyothin, S.; Jacquet, M.; Komalamisra, N.; Apiwathnasorn, C.; Dujardin, J.; Boyer, S. Preserving blood-fed Aedes albopictus from field to laboratory for blood source determination. JITMM2013 Proc. 2014, 3, 31–39. [Google Scholar]
  101. Martínez-de la Puente, J.; Ruiz, S.; Soriguer, R.; Figuerola, J. Effect of blood meal digestion and DNA extraction protocol on the success of blood meal source determination in the malaria vector Anopheles atroparvus. Malar. J. 2013, 12, 109. [Google Scholar] [CrossRef] [Green Version]
  102. Egizi, A.; Healy, S.P.; Fonseca, D.M. Rapid blood meal scoring in anthropophilic Aedes albopictus and application of PCR blocking to avoid pseudogenes. Infect. Genet. Evol. 2013, 16, 122–128. [Google Scholar] [CrossRef]
  103. Muñoz, J.; Eritja, R.; Alcaide, M.; Montalvo, T.; Soriguer, R.C.; Figuerola, J. Host-feeding patterns of native Culex pipiens and invasive Aedes albopictus mosquitoes (Diptera: Culicidae) in urban zones from Barcelona, Spain. J. Med. Entomol. 2011, 48, 956–960. [Google Scholar] [CrossRef] [PubMed]
  104. Hadj-Henni, L.; De Meulemeester, T.; Depaquit, J.; Noël, P.; Germain, A.; Helder, R.; Augot, D. Comparison of vertebrate cytochrome b and prepronociceptin for blood meal analyses in Culicoides. Front. Vet. Sci. 2015, 2. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  105. Tanigawa, M.; Sato, Y.; Ejiri, H.; Imura, T.; Chiba, R.; Yamamoto, H.; Kawaguchi, M.; Tsuda, Y.; Murata, K.; Yukawa, M. Molecular identification of avian Haemosporidia in wild birds and mosquitoes on Tsushima Island, Japan. J. Vet. Med. Sci. 2013, 75, 319–326. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  106. Martínez-de la Puente, J.; Díez-Fernández, A.; Montalvo, T.; Bueno-Marí, R.; Pangrani, Q.; Soriguer, R.C.; Senar, J.C.; Figuerola, J. Do invasive mosquito and bird species alter avian malaria parasite transmission? Diversity 2020, 12, 111. [Google Scholar] [CrossRef] [Green Version]
  107. Kim, H.; Yu, H.M.; Lim, H.W.; Yang, S.-C.; Roh, J.Y.; Chang, K.S.; Shin, E.-H.; Ju, Y.R.; Lee, W.-G. Host-feeding pattern and dengue virus detection of Aedes albopictus (Diptera: Culicidae) captured in an urban park in Korea. J. Asia Pac. Entomol. 2017, 20, 809–813. [Google Scholar] [CrossRef]
  108. Dennett, J.A.; Bala, A.; Wuithiranyagool, T.; Randle, Y.; Sargent, C.B.; Guzman, H.; Siirin, M.; Hassan, H.K.; Reyna-Nava, M.; Unnasch, T.R.; et al. Associations between two mosquito populations and West Nile Virus in Harris County, Texas, 2003–2006 1. J. Am. Mosq. Control Assoc. 2007, 23, 264–275. [Google Scholar] [CrossRef]
  109. Faraji, A.; Egizi, A.; Fonseca, D.M.; Unlu, I.; Crepeau, T.; Healy, S.P.; Gaugler, R. Comparative host feeding patterns of the Asian tiger mosquito, Aedes albopictus, in urban and suburban northeastern USA and implications for disease transmission. PLoS Negl. Trop. Dis. 2014, 8, e3037. [Google Scholar] [CrossRef] [Green Version]
  110. Pereira-dos Santos, T.; Roiz, D.; Santos de Abreu, F.V.; Luz, S.L.B.; Santalucia, M.; Jiolle, D.; Santos Neves, M.S.A.; Simard, F.; Lourenço-de-Oliveira, R.; Paupy, C. Potential of Aedes albopictus as a bridge vector for enzootic pathogens at the urban-forest interface in Brazil. Emerg. Microbes Infect. 2018, 7, 1–8. [Google Scholar] [CrossRef] [Green Version]
  111. Tamashiro, M.; Toma, T.; Mannen, K.; Higa, Y.; Miyagi, I. Bloodmeal identification and feeding habits of mosquitoes (Diptera: Culicidae) collected at five islands in the Ryukyu Archipelago, Japan. Jap. J. Sanit. Zool. 2011, 62, 53–70. [Google Scholar] [CrossRef] [Green Version]
  112. Martínez-de la Puente, J.; Soriguer, R.; Senar, J.; Figuerola, J.; Bueno-Mari, R.; Montalvo, T. Mosquitoes in an urban zoo: Identification of blood meals, flight distances of engorged females, and avian malaria infections. Front. Vet. Sci. 2020, 7, 460. [Google Scholar] [CrossRef]
  113. Kim, K.S.; Tsuda, Y.; Yamada, A. Bloodmeal identification and detection of avian malaria parasite from mosquitoes (Diptera: Culicidae) inhabiting coastal areas of Tokyo Bay, Japan. J. Med. Entomol. 2009, 46, 1230–1234. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  114. Kek, R.; Hapuarachchi, H.C.; Chung, C.-Y.; Humaidi, M.B.; Razak, M.A.B.A.; Chiang, S.; Lee, C.; Tan, C.-H.; Yap, G.; Chong, C.-S.; et al. Feeding hostrange of Aedes albopictus (Diptera: Culicidae) demonstrates its opportunistic host-seeking behavior in rural Singapore. J. Med. Entomol. 2014, 51, 880–884. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  115. Guo, X.-X.; Li, C.-X.; Wang, G.; Zheng, Z.; Dong, Y.-D.; Zhang, Y.-M.; Xing, D.; Zhao, T.-Y. Host feeding patterns of mosquitoes in a rural malaria-endemic region in Hainan Island, China. J. Am. Mosq. Control Assoc. 2014, 30, 309–311. [Google Scholar] [CrossRef] [PubMed]
  116. Stenn, T.; Peck, K.J.; Rocha Pereira, G.; Burkett-Cadena, N.D. Vertebrate hosts of Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus (Diptera: Culicidae) as potential vectors of Zika Virus in Florida. J. Med. Entomol. 2019, 56, 10–17. [Google Scholar] [CrossRef]
  117. Goodman, H.; Egizi, A.; Fonseca, D.M.; Leisnham, P.T.; LaDeau, S.L. Primary blood-hosts of mosquitoes are influenced by social and ecological conditions in a complex urban landscape. Parasites Vectors 2018, 11, 218. [Google Scholar] [CrossRef]
  118. Siriyasatien, P.; Pengsakul, T.; Kittichai, V.; Phumee, A.; Kaewsaitiam, S.; Thavara, U.; Tawatsin, A.; Savadachanukorn, P.A.; Mull, M.S. Identification of blood meal of field caught Aedes aegypti (L.) by multiplex PCR. Southeast Asian J. Trop. Med. Public Health 2010, 41, 43–47. [Google Scholar]
  119. Diallo, D.; Chen, R.; Diagne, C.T.; Ba, Y.; Dia, I.; Sall, A.A.; Weaver, S.C.; Diallo, M. Bloodfeeding patterns of sylvatic arbovirus vectors in southeastern Senegal. Trans. R. Soc. Trop. Med. Hyg. 2013, 107, 200–203. [Google Scholar] [CrossRef] [Green Version]
  120. Barrera, R.; Bingham, A.M.; Hassan, H.K.; Amador, M.; Mackay, A.J.; Unnasch, T.R. Vertebrate hosts of Aedes aegypti and Aedes mediovittatus (Diptera: Culicidae) in rural Puerto Rico. J. Med. Entomol. 2012, 49, 917–921. [Google Scholar] [CrossRef] [Green Version]
  121. Osório, H.C.; Zé-Zé, L.; Alves, M.J. Host-feeding patterns of Culex pipiens and other potential mosquito vectors (Diptera: Culicidae) of West Nile Virus (Flaviviridae) collected in Portugal. J. Med. Entomol. 2012, 49, 717–721. [Google Scholar] [CrossRef]
  122. Schönenberger, A.C.; Wagner, S.; Tuten, H.C.; Schaffner, F.; Torgerson, P.; Furrer, S.; Mathis, A.; Silaghi, C. Host preferences in host-seeking and blood-fed mosquitoes in Switzerland: Host preferences in mosquitoes. Med. Vet. Entomol. 2016, 30, 39–52. [Google Scholar] [CrossRef]
  123. Molaei, G.; Andreadis, T.G.; Armstrong, P.M.; Diuk-Wasser, M. Host-feeding patterns of potential mosquito vectors in Connecticut, USA: Molecular analysis of bloodmeals from 23 species of Aedes, Anopheles, Culex, Coquillettidia, Psorophora, and Uranotaenia. J. Med. Entomol. 2008, 45, 1143–1151. [Google Scholar] [CrossRef]
  124. Molaei, G.; Farajollahi, A.; Scott, J.J.; Gaugler, R.; Andreadis, T.G. Human bloodfeeding by the recently introduced mosquito, Aedes japonicus japonicus, and public health implications. J. Am. Mosq. Control Assoc. 2009, 25, 210–214. [Google Scholar] [CrossRef] [PubMed]
  125. Damiens, D.; Ayrinhac, A.; Van Bortel, W.; Versteirt, V.; Dekoninck, W.; Hance, T. Invasive process and repeated cross-sectional surveys of the mosquito Aedes japonicus japonicus establishment in Belgium. PLoS ONE 2014, 9, e89358. [Google Scholar] [CrossRef] [PubMed]
  126. Anderson, J.F.; Armstrong, P.M.; Misencik, M.J.; Bransfield, A.B.; Andreadis, T.G.; Molaei, G. Seasonal distribution, blood-feeding habits, and viruses of mosquitoes in an open-faced quarry in Connecticut, 2010 and 2011. J. Am. Mosq. Control Assoc. 2018, 34, 1–10. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  127. Montarsi, F.; Drago, A.; Pont, M.D.; Delai, N.; Carlin, S.; Cazzin, S.; Ciocchetta, S.; Arnoldi, D.; Baldacchino, F.; Rizzoli, A.; et al. Current knowledge on the distribution and biology of the recently introduced invasive mosquito Aedes koreicus (Diptera: Culicidae). Atti Accad. Naz. Ital. Entomol. 2015. [Google Scholar] [CrossRef]
  128. Tripepi, L. Preferenze Alimentari di Aedes koreicus, una Nuova Zanzara Invasiva e Implicazioni Nella Trasmissione di Patogeni. Bachelor’s Thesis, University of Padova, Padova, Italy, 2014. [Google Scholar]
  129. Valerio, L.; Marini, F.; Bongiorno, G.; Facchinelli, L.; Pombi, M.; Caputo, B.; Maroli, M.; della Torre, A. Host-feeding patterns of Aedes albopictus (Diptera: Culicidae) in urban and rural contexts within Rome province, Italy. Vector Borne Zoonotic Dis. 2010, 10, 291–294. [Google Scholar] [CrossRef] [PubMed]
  130. Sivan, A.; Shriram, A.N.; Sunish, I.P.; Vidhya, P.T. Host-feeding pattern of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in heterogeneous landscapes of South Andaman, Andaman and Nicobar Islands, India. Parasitol. Res. 2015, 114, 3539–3546. [Google Scholar] [CrossRef] [PubMed]
  131. Crawford, J.E.; Alves, J.M.; Palmer, W.J.; Day, J.P.; Sylla, M.; Ramasamy, R.; Surendran, S.N.; Black, W.C.; Pain, A.; Jiggins, F.M. Population genomics reveals that an anthropophilic population of Aedes aegypti mosquitoes in West Africa recently gave rise to American and Asian populations of this major disease vector. BMC Biol. 2017, 15, 16. [Google Scholar] [CrossRef] [Green Version]
  132. Sardelis, M.R.; Turell, M.J. Ochlerotatus j. japonicus in Frederick county, Maryland: Discovery, distribution, and vector competence for West Nile Virus. J. Am. Mosq. Control Assoc. 2001, 17, 137–141. [Google Scholar]
  133. Rose, N.H.; Sylla, M.; Badolo, A.; Lutomiah, J.; Ayala, D.; Aribodor, O.B.; Ibe, N.; Akorli, J.; Otoo, S.; Mutebi, J.-P.; et al. Climate and urbanization drive mosquito preference for humans. Curr. Biol. 2020, 30, 3570–3579. [Google Scholar] [CrossRef]
  134. Grubaugh, N.D.; Sharma, S.; Krajacich, B.J.; Fakoli III, L.S.; Bolay, F.K.; Diclaro II, J.W.; Johnson, W.E.; Ebel, G.D.; Foy, B.D.; Brackney, D.E. Xenosurveillance: A novel mosquito-based approach for examining the human-pathogen landscape. PLoS Negl. Trop. Dis. 2015, 9, e0003628. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  135. Tomazatos, A.; Jansen, S.; Pfister, S.; Török, E.; Maranda, I.; Horváth, C.; Keresztes, L.; Spînu, M.; Tannich, E.; Jöst, H.; et al. Ecology of West Nile Virus in the Danube Delta, Romania: Phylogeography, xenosurveillance and mosquito host-feeding patterns. Viruses 2019, 11, 1159. [Google Scholar] [CrossRef] [PubMed] [Green Version]
Figure 1. Current known distribution of Aedes invasive species in Europe (September 2020; accessed on 19 November 2020). The maps show the current European distribution of Ae. albopictus (upper-left panel), Ae. aegypti (upper-right panel), Ae. japonicus (lower-left panel) and Ae. koreicus (lower-left panel). European Centre for Disease Prevention and Control and European Food Safety Authority. Mosquito maps (internet). Stockholm: ECDC; 2020. Available from: https://ecdc.europa.eu/en/disease-vectors/surveillance-and-disease-data/mosquito-maps.
Figure 1. Current known distribution of Aedes invasive species in Europe (September 2020; accessed on 19 November 2020). The maps show the current European distribution of Ae. albopictus (upper-left panel), Ae. aegypti (upper-right panel), Ae. japonicus (lower-left panel) and Ae. koreicus (lower-left panel). European Centre for Disease Prevention and Control and European Food Safety Authority. Mosquito maps (internet). Stockholm: ECDC; 2020. Available from: https://ecdc.europa.eu/en/disease-vectors/surveillance-and-disease-data/mosquito-maps.
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Figure 2. Percentage of blood meals derived from different vertebrate hosts. Percentage of feedings derived from humans (black), other mammals (blue), bird (red) and other vertebrates including reptiles, amphibians and fish (green) for the four Aedes invasive species studied (a) in both their native and invaded ranges or (b) studies conducted only in Europe. Blood meals derived from more than one vertebrate species (i.e., mixed blood meals) were excluded from the plot. The studies used for this figure are listed in the Supplementary Material Table S1.
Figure 2. Percentage of blood meals derived from different vertebrate hosts. Percentage of feedings derived from humans (black), other mammals (blue), bird (red) and other vertebrates including reptiles, amphibians and fish (green) for the four Aedes invasive species studied (a) in both their native and invaded ranges or (b) studies conducted only in Europe. Blood meals derived from more than one vertebrate species (i.e., mixed blood meals) were excluded from the plot. The studies used for this figure are listed in the Supplementary Material Table S1.
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Table 1. Vertebrate hosts of the four invasive Aedes species identified using molecular methods.
Table 1. Vertebrate hosts of the four invasive Aedes species identified using molecular methods.
Aedes albopictusFamilySpeciesReferences
MammalsHominidaeHomo sapiens[73,84,97,103,105,106], [107] *, [108,109,110,111,112,113], [114] *, [115], [116] *, [117]
CanidaeCanis lupus **[97,108,109,110,113], [114] *, [115,117]
FelidaeFelis silvestris **[97,105,106], [107] *, [109], [114] *, [117]
ProcyonidaeProcyon lotor[97], [116] *
MuridaeRattus norvegicus[110,113], [116] *, [117]
Mus musculus[105]
CricetidaePeromyscus leucopus[109]
SciuridaeSciurus carolinensis[111]
LeporidaeSylvilagus floridanus[109], [116] *
SuidaeSus **[108,111], [114] *, [115]
BovidaeBos taurus[73]
CervidaeOdocoileus virginianus[117]
EquidaeEquus caballus[97]
SoricidaeSuncus murinus[111]
ErinaceidaeErinaceus europaeus[84]
DasypopidaeDasypus novemcintus[116] *
PhyllostomidaeTonatia bidens[110]
DidelphidaeDidelphis virginiana[97,109]
Birds
PhasianidaeGallus domesticus[97,115]
TurdidaeTurdus merula[84]
PasseridaePasser montanus[84]
TamnophilideTaraba major[110]
CardinalidaeCardinalis cardinalis[97]
AnatidaeUnknown[115]
Aedes aegypti
MammalsHominidaeHomo sapiens[92,116,118,119,120,121]
CanidaeCanis lupus **[92,116,118,120]
FelidaeFelis silvestris **[92,120]
BovidaeBos taurus[92,118]
SuidaeSus scrofa **[118]
EquidaeEquus caballus[120]
Birds
PhasianidaeGallus domesticus[120]
PhasianidaeFrancolinus squamatus[119]
MimidaeMimus polyglottos[116]
MusophagidaeCrinifer piscator[119]
Aedes japonicus
MammalsHominidaeHomo sapiens[81,117,122,123,124,125]
CanidaeCanis lupus **[122]
Canis latrans[126]
FelidaeFelis silvestris **[117]
Panthera leo persica[122]
ProcyonidaeProcyon lotor[126]
PhocidaePhoca vitulina[122]
MuridaeRattus norvegicus[117]
SciuridaeUnknown species[123]
CamelidaeLama sp.[122]
BovidaeBos taurus[125]
Boselaphus tragocamelus[122]
Ovis sp.[122]
CervidaeDama dama[124]
Odocoileus virginianus[123,124,126]
EquidaeEquus caballus[81,124]
Equus asinus[122]
DidelphidaeDidelphis virginiana[124]
Birds
PhasianidaeGallus domesticus[122]
TurdidaeTurdus merula[122]
PasseridaePasser domesticus[122]
SpheniscidaeSpheniscus humboldti[122]
RheidaeRhea pennata[122]
Aedes koreicus
MammalsHominidaeHomo sapiens[127,128]
CanidaeCanis lupus **[127,128]
BovidaeBos taurus[128]
* These also reported blood meal hosts such as “amphibian”, “fish” or “turtle”. ** The identification of blood from Canis lupus, Sus scrofa and Felis silvestris may correspond to domestic animals.
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Cebrián-Camisón, S.; Martínez-de la Puente, J.; Figuerola, J. A Literature Review of Host Feeding Patterns of Invasive Aedes Mosquitoes in Europe. Insects 2020, 11, 848. https://doi.org/10.3390/insects11120848

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Cebrián-Camisón S, Martínez-de la Puente J, Figuerola J. A Literature Review of Host Feeding Patterns of Invasive Aedes Mosquitoes in Europe. Insects. 2020; 11(12):848. https://doi.org/10.3390/insects11120848

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Cebrián-Camisón, Sonia, Josué Martínez-de la Puente, and Jordi Figuerola. 2020. "A Literature Review of Host Feeding Patterns of Invasive Aedes Mosquitoes in Europe" Insects 11, no. 12: 848. https://doi.org/10.3390/insects11120848

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