Increased Probability of Cell Membrane Breakdown by Use of Microfluidic System

Thanaphat Thonapan; Boonchai Techaumnat; Adisorn Tuantranont

doi:10.4028/www.scientific.net/AMM.781.620

Paper Titles

Analysis of Electromyography in Dynamic Hand Motions Using L-Kurtosis
p.604

Modeling of Gelatin-Based Head Phantom Based on its Electrical Properties for Wideband Microwave Imaging Application
p.608

Simulation of Electric Field in Co-Field Treatment Chamber Using FEM
p.612

Chromatin Detection in Malaria Thick Blood Film Using Automated Image Processing
p.616

Increased Probability of Cell Membrane Breakdown by Use of Microfluidic System
p.620

Heart Disease Classification Using Artificial Neural Networks
p.624

Prediction Modeling of PM-10 in Chiangmai City Moat by Using Artificial Neural Networks
p.628

Mammogram Enhancement Using Wavelet Transform and Sigmoid Function
p.632

Incomplete Nucleic Acid Sequences Visualization: A Case Study in Virus Sequences
p.637

HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vol. 781Increased Probability of Cell Membrane Breakdown...

Increased Probability of Cell Membrane Breakdown by Use of Microfluidic System

Abstract:

This research shows the experiment results of cell membrane breakdown. We did the experiments by using plant protoplasts in a microfluidic system to increase the efficiency of membrane charging process. In the experiment, we apply 5 MHz of sine wave to attract the cell to target position and then apply 10–200 kHz to make breakdown occur. Under the experiment conditions (R = 10–25 μm, U = 10 V_P, t_L = 10 ms, σ = 45–55 mS/m), the use of microfluidic system with insulating wall and orifice increased breakdown probability. Breakdown probability is 86.8%, and the probabilities of f_L at 10–100 kHz are higher than the probability of f_L at 200 kHz.