Effect of Oxidized Myofibrils Protein Subjected to Mutiple Freeze-Thaw Cycles on N-Nitrosamine Formation in In Vitro Model System

Hua Yang; Pei Pei Meng; Rui Wang; Pei Ran Li; Peng Li; Chang Lu Wang; Li Zhen Ma

doi:10.4028/www.scientific.net/AMR.550-553.1590

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 550-553Effect of Oxidized Myofibrils Protein Subjected to...

Effect of Oxidized Myofibrils Protein Subjected to Mutiple Freeze-Thaw Cycles on N-Nitrosamine Formation in In Vitro Model System

Abstract:

N-nitrosamine is a kind of carcinogenic substance, which is possibly formed in the reaction of nitrites with amino acids or secondary amines. Two in vitro model systems were designed to evaluate the influence of oxidized myofibrils protein subjected to repeated freeze-thaw cycles (0, 1, 2, 3, 4, 7, 10 times) on N-nitrosamine formation. Model system I contains diethylamine and sodium nitrite, while model system II contains only sodium nitrite as reaction solution. Oxidized myofibrils protein were added to both systems. The results revealed that as the number of freeze-thaw cycles increased, cross-linking of myosin heavy chains and the content of protein carbonyl increased, but the content of protein sulfydryl decreased, which indicates oxidization of protein occurred. The concentration of N-nitrosodiethylamine increased as the number of freeze-thaw cycles increased, especially after four cycles. Oxidized myofibrils protein promoted the formation of N-nitrosodiethylamine. The more the times of freeze-thaw cycles were subjected, the more oxidization of myofibrils protein occurred and the higher yield of the N-nitrosodiethylamine.

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Periodical:

Advanced Materials Research (Volumes 550-553)

Pages:

1590-1594

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.550-553.1590

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Online since:

July 2012

Authors:

Hua Yang, Pei Pei Meng, Rui Wang, Pei Ran Li, Peng Li, Chang Lu Wang, Li Zhen Ma

Keywords:

Freeze-Thaw Cycle, Model System, N-Nitrosodiethylamine, Oxidized Myofibrils Protein, Porcine Longissimus Dorsi

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