Application of Glycosylated Nitrosohemoglobin in Meat Batters: Color Formation and Antimicrobial Activity

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Abstract:

Sodium nitrite is an important food additive in meat industry. However, because of its carcinogenicity, the studies about nitrite substitutes have been focused on for many years. In this study, glycosylated nitrosohemoglobin (G-NO-Hb) synthesized by porcine blood, nitrite and sugar through maillard reaction was applied in chilled meat batters to replace for nitrite. Color difference, total aerobic plate count (APC) and total volatile basic nitrogen (TVB-N) were determined in order to evaluate the quality of meat batters. The identified results by UV-Vis spectra analysis showed that the produced pigment products by porcine blood, nitrite and sugar through maillard reaction was NO-Hb because they had special absorption peaks at 420, 542 and 578 nm. The a*-values of batters treated with G-NO-Hb showed a significant increase (P 0.05), which indicated that the addition of G-NO-Hb had a positive effect on the red color formation of pork batters. Also, both TVB-N values and APC of samples treated with G-NO-Hb were significantly lower than the control samples (P < 0.05). It revealed that G-NO-Hb was a potential substance to substitute for nitrite during meat curing.

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Periodical:

Advanced Materials Research (Volumes 554-556)

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1057-1063

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July 2012

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[1] R. A. Mancini and M. C. Hunt, Current research in meat color, MEAT SCI. 71 (2005) 100-121.

DOI: 10.1016/j.meatsci.2005.03.003

Google Scholar

[2] I. Cho and L. Bratzler, Effect of sodium nitrite on flavor of cured pork, J FOOD SCI. 35 (1970) 668-670.

DOI: 10.1111/j.1365-2621.1970.tb04839.x

Google Scholar

[3] A. Al-Shuibi and B. Al-Abdullah, Substitution of nitrite by sorbate and the effect on properties of mortadella, MEAT SCI. 62 (2002) 473-478.

DOI: 10.1016/s0309-1740(02)00041-4

Google Scholar

[4] J. E. Stuff, E. T. Goh, S. L. Barrera, M. L. Bondy, and M. R. Forman, N-Nitroso Compounds: Assessing Agreement between Food Frequency Questionnaires and 7-Day Food Records, J AM DIET ASSOC. 109 (2009) 1179-1183.

DOI: 10.1016/j.jada.2009.04.006

Google Scholar

[5] K. O. Honikel, The use and control of nitrate and nitrite for the processing of meat products, MEAT SCI. 78 (2008) 68-76.

DOI: 10.1016/j.meatsci.2007.05.030

Google Scholar

[6] F. Shahidi and R. B. Pegg, Colour characteristics of cooked cured-meat pigment and its application to meat, FOOD CHEM. 38 (1990) 61-68.

DOI: 10.1016/0308-8146(90)90206-j

Google Scholar

[7] M. Deda, J. Bloukas, and G. Fista, Effect of tomato paste and nitrite level on processing and quality characteristics of frankfurters, MEAT SCI. 76 (2007) 501-508.

DOI: 10.1016/j.meatsci.2007.01.004

Google Scholar

[8] S. Zarringhalami, M. Sahari, and Z. Hamidi-Esfehani, Partial replacement of nitrite by annatto as a color additive in sausage, MEAT SCI. 81 (2009) 281-284.

DOI: 10.1016/j.meatsci.2008.08.003

Google Scholar

[9] X. Zhang, B. Kong, and Y. L. Xiong, Production of cured meat color in nitrite-free Harbin red sausage by Lactobacillus fermentum fermentation, MEAT SCI. 77 (2007) 593-598.

DOI: 10.1016/j.meatsci.2007.05.010

Google Scholar

[10] F. Shahidi and R. B. Pegg, Nitrite-free meat curing systems: update and review, FOOD CHEM. 43 (1992) 185-191.

DOI: 10.1016/0308-8146(92)90171-w

Google Scholar

[11] F. SHAHIDI and R. B. PEGG, Novel Synthesis of Cooked Cured‐Meat Pigment, J FOOD SCI. 56 (1991) 1205-1208.

DOI: 10.1111/j.1365-2621.1991.tb04735.x

Google Scholar

[12] S. J. Millar, B. W. Moss, and M. H. Stevenson, Some observations on the absorption spectra of various myoglobin derivatives found in meat, MEAT SCI. 42 (1996) 277-288.

DOI: 10.1016/0309-1740(94)00045-x

Google Scholar

[13] K. Arihara, H. Kushida, Y. Kondo, M. Itoh, J. B. Luchansky, and R. G. Cassens, Conversion of Metmyoglobin to Bright Red Myoglobin Derivatives by Chromobacterium violaceum, Kurthia sp., and Lactobacillus fermenturn JCM1173, J FOOD SCI. 58 (1993) 38-42.

DOI: 10.1111/j.1365-2621.1993.tb03205.x

Google Scholar

[14] J. Xu and W. Verstraete, Evaluation of nitric oxide production by lactobacilli, Appl Microbiol Biotechnol. 56 (2001) 504-507.

Google Scholar

[15] B. N. MCCLURE, J. G. SEBRANEK, Y. H. KIM, and G. A. SULLIVAN, The effects of lactate on nitrosylmyoglobin formation from nitrite and metmyoglobin in a cured meat system, FOOD CHEM. 129 (2011) 1072-1079.

DOI: 10.1016/j.foodchem.2011.05.077

Google Scholar

[16] J. Botta, J. Lauder, and M. Jewer, Effect of Methodology on Total Volatile Basic Nitrogen (TVB‐N) Determination as an Index of Quality of Fresh Atlantic Cod (Gadus morhua), J FOOD SCI. 49 (1984) 734-736.

DOI: 10.1111/j.1365-2621.1984.tb13197.x

Google Scholar

[17] J. Chasco, G. Lizaso, and M. Beriain, Cured colour development during sausage processing, MEAT SCI. 44 (1996) 203-211

DOI: 10.1016/s0309-1740(96)00092-7

Google Scholar

[18] M. Song and B. Kong, Extending shelf life of chilled pork by combination of Nisin and other preservatives, J Northeast AGR University. 10 (2008) 82-88.In Chinese.

Google Scholar

[19] A. Akköse and N. Aktas, Determination of glass transition temperature of beef and effects of various cryoprotective agents on some chemical changes, MEAT SCI. 80 (2008) 875-878.

DOI: 10.1016/j.meatsci.2008.04.006

Google Scholar

[20] M. Daglia, M. T. Cuzzoni, and C. Dacarro, Antibacterial activity of coffee, J AGR FOOD CHEM. 42 (1994) 2270-2272.

DOI: 10.1021/jf00046a035

Google Scholar

[21] J. A. Rufián-Henares and S. P. de la Cueva, Antimicrobial Activity of Coffee Melanoidins- A Study of Their Metal-Chelating Properties, J AGR FOOD CHEM. 57 (2009) 432-438.

DOI: 10.1021/jf8027842

Google Scholar

[22] P. Dutta, S. Tripathi, G. Mehrotra, and J. Dutta, Perspectives for chitosan based antimicrobial films in food applications, FOOD CHEM. 114 (2009) 1173-1182.

DOI: 10.1016/j.foodchem.2008.11.047

Google Scholar