Paper Titles

Overview of Progress in Development of the Bionic Underwater Propulsion System
p.9

A Method for Optimal Synthesis of a Biomimetic Four-Bar Linkage Knee Joint for a Knee-Ankle-Foot Orthosis
p.20

A Three Dimensional Finite Element Analysis of Mechanical Stresses in the Human Knee Joint: Problem of Cartilage Destruction
p.29

Biomechanical Comparison between Two Models of the Lumbar Intersomatic Fusion Cage Analyzed by the Finite Element Method
p.40

The Effect of Sintering Temperature to the Quality of Hydroxyapatite Coating on Cobalt Alloys as the Candidate of Bone Implant Prosthesis
p.59

Eggshell Membrane Protein Modified Silk Fibroin-Poly Vinyl Alcohol Scaffold for Bone Tissue Engineering: In Vitro and In Vivo Study
p.69

Composite of Chitosan-Collagen-Aloe vera for Scaffolds Application on Skin Tissue
p.82

PLGA-Collagen Composite Synthesis with the Antibacterial Addition of Jatropha curcas L. Leaf Extract as Absorbable Surgical Suture Candidate
p.90

Prediction of Phagocyte Transmigration for Foreign Body Responses to Subcutaneous Biomaterial Implantations Using Differential Transform Method
p.98

HomeJournal of Biomimetics, Biomaterials and...Journal of Biomimetics, Biomaterials and...Composite of Chitosan-Collagen-Aloe vera for...

Composite of Chitosan-Collagen-Aloe vera for Scaffolds Application on Skin Tissue

Article Preview

Abstract:

In case of burns, scaffolds serve as cover for burns and facilitate cells regeneration. Accordingly, this study seeks procedures to synthesize composite scaffolds for burns utilizing collagen-chitosan-Aloe vera to find the best concentration of Aloe vera as scaffolds application. The synthesis of scaffolds was performed with collagen constituent composition as follow: chitosan (1:1) was dissolved in 0.05M Acetic Acid, then subsequent variations of Aloe vera (AV), namely 0% AV; 0.1% AV; 0.15% AV; 0.2% AV; and 0.25% AV were added into collagen-chitosan solution, and freeze dry method was applied. Sample characterization was done by FTIR, tensile strength test, SEM test, cytotoxicity assay and degradation test. Typical absorption bands of collagen in the FTIR test was obtained at 1645.5 cm^-1 (C = O stretch), chitosan at 1540.28 cm^-1 (NH stretch) and Aloe vera at 3474.41 cm^-1 (NH stretch). Tensile test data showed the highest number of tensile strength from the 0.1% AV sample at 0.017 MPa. The SEM test revealed a pore size of <50 μm. During cytotoxicity assay, the entire sample is not toxic. Degradation test demonstrated that overall sample was not exhausted within two weeks. In conclusion, sample with 0.2% AV was potential as scaffolds for burns skin tissue.

You might also be interested in these eBooks

Journal of Biomimetics, Biomaterials and Biomedical Engineering Vol. 32

Info:

Periodical:

Journal of Biomimetics, Biomaterials and Biomedical Engineering (Volume 32)

Pages:

82-89

DOI:

https://doi.org/10.4028/www.scientific.net/JBBBE.32.82

Citation:

Cite this paper

Online since:

May 2017

Authors:

Andini Isfandiary, Prihartini Widiyanti*, Dyah Hikmawati

Keywords:

Aloe vera, Burns, Chitosan, Collagen, Scaffold

Export:

RIS, BibTeX

Price:

Permissions:

Request Permissions

* - Corresponding Author

[1] Sontheimer RD. Skin is not the largest organ. J Invest Dermatol. 2014; 134(2): 581 –2. doi: 10. 1038/jid. 2013. 335.

[2] Singh, D.; Tripathi, A.; Zo, S.M.; Singh, D.; Han, S.S. Synthesis of composite gelatin-hyaluronic acid-alginate porous scaffold and evaluation for in vitro stem cell growth and in vivo tissue integration. Colloid Surf. B 2014, 116, 502–509.

DOI: 10.1016/j.colsurfb.2014.01.049

[3] Shevchenko RV, James SL, James SE. A review of tissue-engineered skin bioconstructs available for skin reconstruction. J R Soc Interface. 2010; 7(43): 229–58. doi: 10. 1098/rsif. 2009. 0403.

DOI: 10.1098/rsif.2009.0403

[4] Puente, P.D.; Ludena, D. Cell culture in autologous fibrin scaffolds for applications in tissue engineering. Exp. Cell. Res. 2014, 322, 1–11.

DOI: 10.1016/j.yexcr.2013.12.017

[5] Matsuura, K.; Utoh, R.; Nagase, K.; Okano, T. Cell sheet approach for tissue engineering and regenerative medicine. J. Control. Release 2014, 190, 228–239.

DOI: 10.1016/j.jconrel.2014.05.024

[6] Qadir M. Imran. 2009. Medicinal and Cosmetological Importance of Aloe vera. International Journal of Natural Therapy vol. 02, pp.21-26.

[7] de Oliveira, E. T., Crocomo, O. J., Farinha, T. B., and Gallo, L. A, 2009. Large-scale micropropagation of Aloe vera. HortScience 44, 1675–1678.

DOI: 10.21273/hortsci.44.6.1675

[8] Rose Chellan., Jithendra Panneerselvam., Rajam Abraham Merlin., Kalaivani Tambiran., Mandal Asit Baran. 2013. Preparation and characterization of Aloe vera blended Collagen Chitosan Composite scaffold for tissue engineering applications. Journal of Applied Materials and Interfaces.

DOI: 10.1021/am401637c

[9] Peng H, Tan L, Osaki M, Zhan Y, Ijiri K, Tsuchimochi K et al., 2008. ESE-1 is a potent repressor of type II collagen gene (COL2A1) transcription in human chondrocytes. J Cell Physiol 215: 562–73.

DOI: 10.1002/jcp.21338

[10] Borjesson L, Willen R, Haboubi N, Duff SE, Hulten L. The risk of dysplasia and cancer in the ileal pouch mucosa after restorative proctocolectomy for ulcerative proctocolitis is low: a long-term follow-up study. Colorectal Dis 2004; 6: 494–8.

DOI: 10.1111/j.1463-1318.2004.00716.x

[11] Spielmann H, et. al. 2007. The practical application of three validated in vitro embryotoxicity tests. ATLA, 527-538.

[12] Kush Kumar Nayak, Pratima Gupta, 2015. In vitro biocompatibility study of keratin/agar scaffold for tissue engineering. International Journal of Biological Macromolecules 81 (2015) 1–10.

DOI: 10.1016/j.ijbiomac.2015.07.025

[13] Linbo Wu, Jiandong Ding, 2004. In vitro degradation of three-dimensional porous poly(d, l-lactide-co-glycolide) scaffolds for tissue engineering. B 283. iomaterials, Volume 25, Issue 27, December 2004, Pages 5821–5830.

DOI: 10.1016/j.biomaterials.2004.01.038

[14] Joseph R. Davis, 2004. Tensile Testing, 2nd Edition. ASM International, p.283.

[15] Bhuvana KB, Hema NG, Rajesh TP, 2014. Review on Aloe Vera. International Journal of Advanced Research (2014), Volume 2, Issue 3, 677-691.

[16] Hui -Min Wang, Yi-Ting Chou, Zhi-Hong Wen, Zhao-Ren Wang, Chun-Hong Chen, Mei-Ling Ho, 2013. Novel Biodegradabl e Porous Scaffold Applied to Skin Regeneration. Plos One, Volume 8, Issue 6, e56330, pp.1-11.

DOI: 10.1371/journal.pone.0056330

[17] O'Brien FJ, Harley BA, Yannas IV, Gibson L, 2004. Influence of freezing rate on pore structure in freeze-dried collagen-GAG scaffolds. Biomaterials. 2004 Mar; 25(6): 1077-86.

DOI: 10.1016/s0142-9612(03)00630-6

[18] Tangsadthakun C, Kanokpanont S , Sanchavanakit N, Banaprasert T, Damrongsakkul S, 2006. Properties of Collagen/Chitosan Scaffolds for Skin Tissue Engineering. Journal of Metals, Materials and Minerals. Vol. 16 No. 1 pp.37-44, (2006).

DOI: 10.1163/156856207779116694

[19] El-Sayed YS, 2016. Time Course of Histomorphologic Features during Chronic Burn Wound Healing. Forensic Medicine and Anatomy Research, 2016, 4, 1-6.

DOI: 10.4236/fmar.2016.41001