Osteoblastic Cell Differentiation on BMP-2 Pre-Adsorbed Octacalcium Phosphate and Hydroxyapatite

Takashi Kumagai; Takahisa Anada; Yoshitomo Honda; Masamichi Takami; Ryutaro Kamijyo; Hidetoshi Shimauchi; Osamu Suzuki

doi:10.4028/www.scientific.net/KEM.361-363.1025

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EP4 Agonist Accelerates Osteoinduction and Degradation of β-Tricalcium Phosphate in Canine Model
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Osteoblastic Cell Differentiation on BMP-2 Pre-Adsorbed Octacalcium Phosphate and Hydroxyapatite
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Effect of Acid Treated Hydroxyapatite on Osteoblast Maturation
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Comparison of HAp and β-TCP in BMP-2 Dose-Response and Release Study
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HomeKey Engineering MaterialsKey Engineering Materials Vols. 361-363Osteoblastic Cell Differentiation on BMP-2...

Osteoblastic Cell Differentiation on BMP-2 Pre-Adsorbed Octacalcium Phosphate and Hydroxyapatite

Abstract:

The present study was designed to investigate whether bone morphogenetic protein-2 (BMP-2) adsorbed onto octacalcium phosphate (OCP) and hydroxyapatite (HA) surfaces influences osteoblastic cell differentiation. Osteoblastic cell line, UAMS32, were cultured on plastic plate that was coated of synthetic OCP and HA. BMP-2 was pre-adsorbed on these calcium phosphates at 4°C or room temperature for 24h. The cells more proliferated on the BMP-2 pre-adsorbed OCP and HA than that of the controls (OCP and HA coating without BMP-2). ALP activities were higher on HA than on OCP when BMP-2 were pre-adsorbed at 4°C than at room temperature. The results suggest that pre-adsorption of BMP-2 in different temperature affects osteoblastic cell differentiation, most probably through different adsorption state of BMP-2 on this calcium phosphate.

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Periodical:

Key Engineering Materials (Volumes 361-363)

Pages:

1025-1028

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.361-363.1025

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Online since:

November 2007

Authors:

Takashi Kumagai, Takahisa Anada, Yoshitomo Honda, Masamichi Takami, Ryutaro Kamijyo, Hidetoshi Shimauchi, Osamu Suzuki

Keywords:

BMP-2, Differentiation, Octacalcium Phosphate

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