Effect of pH on Hydroxyapatite Formation in Amino Acid Capped Gold Nanoparticles

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Abstract:

The gold nanoparticles were successfully synthesized using the chemical reduction method. The surface of gold nanoparticles was modified using three different charges of amino acid. The amino acid that used in this study was cysteine, arginine, and glutamic acid. The calcium and phosphate sources were added to amino acid capped gold nanoparticle solution to obtain hydroxyapatite. Hydroxyapatite is an inorganic material for teeth and bone tissue engineering. The morphological properties of the samples were investigated using Scanning Electron Microscope (SEM) and Field Emission Scanning Electron Microscope (FE-SEM). Different morphological characteristic was seen in different pH synthesis. The pH variation was normal pH that form in the solution and pH adjusted to 10 using NaOH in the hydroxyapatite formation. The elemental composition was also investigated using Energy-Dispersive X-ray (EDS) analysis. The elemental mapping was shown the homogeneity composition distribution of Ca and P on the sample pH adjustment that contains cysteine. The EDS results indicate the adjusted pH to 10 was potentially formed hydroxyapatite with a Ca/P ratio close to the theory.

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September 2022

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[1] Lima D, Inaba J, Clarindo Lopes L, Calaça G N, Los Weinert P, Lenzi Fogaça R, Ferreira de Moura J, Magalhães Alvarenga L, Cavalcante de Figueiredo B, Wohnrath K and Andrade Pessôa C 2019 Label-free impedimetric immunosensor based on arginine-functionalized gold nanoparticles for detection of DHEAS, a biomarker of pediatric adrenocortical carcinoma Biosens. Bioelectron. 133 86–93.

DOI: 10.1016/j.bios.2019.02.063

Google Scholar

[2] Rawat K A and Kailasa S K 2014 Visual detection of arginine, histidine and lysine using quercetin-functionalized gold nanoparticles Microchim. Acta 181 1917–29.

DOI: 10.1007/s00604-014-1294-6

Google Scholar

[3] Havaldar D V, Patil R V, Moholkar D N, Magdum P S, Vadrale A P and Pawar K D 2020 Differently synthesized gold nanoparticles respond differently to functionalization with L-amino acids Particuology 52 97–104.

DOI: 10.1016/j.partic.2019.12.010

Google Scholar

[4] Tomoaia G, Frangopol P T, Horovitz O, Boboş L-D, Mocanu A and Tomoaia-Cotisel M 2011 The effect of arginine on gold nanoparticles in colloidal solutions and in thin films. J. Nanosci. Nanotechnol. 11 7762–70.

DOI: 10.1166/jnn.2011.4734

Google Scholar

[5] Bansal S A, Kumar V, Karimi J, Singh A P and Kumar S 2020 Role of gold nanoparticles in advanced biomedical applications Nanoscale Adv. 2 3764–87.

DOI: 10.1039/d0na00472c

Google Scholar

[6] Ghodake G, Vassiliadis V S, Choi J-H, Jang J and Lee D S 2015 Facile Synthesis of Gold Nanoparticles by Amino Acid Asparagine: Selective Sensing of Arsenic. J. Nanosci. Nanotechnol. 15 7235–9.

DOI: 10.1166/jnn.2015.10561

Google Scholar

[7] Daruich De Souza C, Ribeiro Nogueira B and Rostelato M E C M 2019 Review of the methodologies used in the synthesis gold nanoparticles by chemical reduction J. Alloys Compd. 798 714–40.

DOI: 10.1016/j.jallcom.2019.05.153

Google Scholar

[8] Vijayakumar S and Ganesan S 2012 In Vitro Cytotoxicity Assay on Gold Nanoparticles with Different Stabilizing Agents ed X Li J. Nanomater. 2012 734398.

DOI: 10.1155/2012/734398

Google Scholar

[9] Bischoff R and Schlüter H 2012 Amino acids: Chemistry, functionality and selected non-enzymatic post-translational modifications J. Proteomics 75 2275–96.

DOI: 10.1016/j.jprot.2012.01.041

Google Scholar

[10] Zarabi M F, Arshadi N, Farhangi A and Akbarzadeh A 2014 Preparation and characterization of gold nanoparticles with amino acids, examination of their stability. Indian J. Clin. Biochem. 29 306–14.

DOI: 10.1007/s12291-013-0358-4

Google Scholar

[11] Shellaiah M, Thirumalaivasan N, Sun K W and Wu S-P 2021 A pH cooperative strategy for enhanced colorimetric sensing of Cr(III) ions using biocompatible L-glutamic acid stabilized gold nanoparticles Microchem. J. 160 105754.

DOI: 10.1016/j.microc.2020.105754

Google Scholar

[12] Wu J, Zhao X, Zou Y, Wu X, Bai W and Zeng X 2021 Electrochemical determination of diethylstilbestrol in livestock and poultry meats by L-cysteine/gold nanoparticles modified electrode Microchem. J. 164 105952.

DOI: 10.1016/j.microc.2021.105952

Google Scholar

[13] Rautaray D, Mandal S and Sastry M 2005 Synthesis of Hydroxyapatite Crystals Using Amino Acid-Capped Gold Nanoparticles as a Scaffold Langmuir 21 5185–91.

DOI: 10.1021/la048541f

Google Scholar

[14] Rodríguez-Lugo V, Karthik T V K, Mendoza-Anaya D, Rubio-Rosas E, Villaseñor Cerón L S, Reyes-Valderrama M I and Salinas-Rodríguez E 2022 Wet chemical synthesis of nanocrystalline hydroxyapatite flakes: effect of pH and sintering temperature on structural and morphological properties R. Soc. Open Sci. 5 180962.

DOI: 10.1098/rsos.180962

Google Scholar

[15] Chauhan N and Singh Y 2021 L-histidine controls the hydroxyapatite mineralization with plate-like morphology: Effect of concentration and media Mater. Sci. Eng. C 120 111669.

DOI: 10.1016/j.msec.2020.111669

Google Scholar

[16] Sari Y W, Saputra A, Bahtiar A and Nuzulia N A 2021 Effects of microwave processing parameters on the properties of nanohydroxyapatite: Structural, spectroscopic, hardness, and toxicity studies Ceram. Int. 47 30061–70.

DOI: 10.1016/j.ceramint.2021.07.182

Google Scholar

[17] Goh K W, Wong Y H, Ramesh S, Chandran H, Krishnasamy S, Ramesh S, Sidhu A and Teng W D 2021 Effect of pH on the properties of eggshell-derived hydroxyapatite bioceramic synthesized by wet chemical method assisted by microwave irradiation Ceram. Int. 47 8879–87.

DOI: 10.1016/j.ceramint.2020.12.009

Google Scholar

[18] Wang Z, Xu Z, Zhao W and Sahai N 2015 A potential mechanism for amino acid-controlled crystal growth of hydroxyapatite J. Mater. Chem. B 3 9157–67.

DOI: 10.1039/c5tb01036e

Google Scholar

[19] Saranya S, Samuel Justin S J, Vijay Solomon R and Wilson P 2018 l-arginine directed and ultrasonically aided growth of nanocrystalline hydroxyapatite particles with tunable morphology Colloids Surfaces A Physicochem. Eng. Asp. 538 270–9.

DOI: 10.1016/j.colsurfa.2017.11.012

Google Scholar

[20] Sari Y W, Tsalsabila A, Darmawan N and Herbani Y 2022 Hydroxyapatite formation under calcium-deficient concentration conditions modulated by amino acid-capped gold nanoparticles Ceram. Int.

DOI: 10.1016/j.ceramint.2022.01.247

Google Scholar

[21] Garg N, Bera S, Rastogi L, Ballal A and Balaramakrishna M V 2020 Synthesis and characterization of L-asparagine stabilised gold nanoparticles: Catalyst for degradation of organic dyes Spectrochim. Acta Part A Mol. Biomol. Spectrosc. 232 118126.

DOI: 10.1016/j.saa.2020.118126

Google Scholar

[22] Wrigglesworth E G and Johnston J H 2021 Mie theory and the dichroic effect for spherical gold nanoparticles: an experimental approach Nanoscale Adv. 3 3530–6.

DOI: 10.1039/d1na00148e

Google Scholar

[23] Ahmad K, Kakakhel M B, Hayat S, Wazir-ud-Din M, Mahmood M M, Ur Rehman S, Siddique M T and Mirza S M 2021 Thermoluminescence study of pellets prepared using NaCl from Khewra Salt Mines in Pakistan Radiat. Environ. Biophys. 60 365–75.

DOI: 10.1007/s00411-021-00894-x

Google Scholar

[24] Vukomanović M, Logar M, Škapin S D and Suvorov D 2014 Hydroxyapatite/gold/arginine: designing the structure to create antibacterial activity J. Mater. Chem. B 2 1557–64.

DOI: 10.1039/c3tb21612h

Google Scholar

[25] Molino G, Palmieri M C, Montalbano G, Fiorilli S and Vitale-Brovarone C 2020 Biomimetic and mesoporous nano-hydroxyapatite for bone tissue application: a short review Biomed. Mater. 15 22001.

DOI: 10.1088/1748-605x/ab5f1a

Google Scholar

[26] Chai Y, Nishikawa M and Tagaya M 2018 Preparation of gold/hydroxyapatite hybrids using natural fish scale template and their effective albumin interactions Adv. Powder Technol. 29 1198–203.

DOI: 10.1016/j.apt.2018.02.011

Google Scholar

[27] Senra M R, Lima R B de, Souza D de H S, Marques M de F V and Monteiro S N 2020 Thermal characterization of hydroxyapatite or carbonated hydroxyapatite hybrid composites with distinguished collagens for bone graft J. Mater. Res. Technol. 9 7190–200.

DOI: 10.1016/j.jmrt.2020.04.089

Google Scholar

[28] Huang L-H, Sun X-Y and Ouyang J-M 2019 Shape-dependent toxicity and mineralization of hydroxyapatite nanoparticles in A7R5 aortic smooth muscle cells Sci. Rep. 9 18979.

DOI: 10.1038/s41598-019-55428-9

Google Scholar

[29] Ramesh S, Tan C Y, Hamdi M, Sopyan I and Teng W D 2007 The influence of Ca/P ratio on the properties of hydroxyapatite bioceramics Proc.SPIE vol 6423.

DOI: 10.1117/12.779890

Google Scholar

[30] Agbeboh N I, Oladele I O, Daramola O O, Adediran A A, Olasukanmi O O and Tanimola M O 2020 Environmentally sustainable processes for the synthesis of hydroxyapatite Heliyon 6 e03765.

DOI: 10.1016/j.heliyon.2020.e03765

Google Scholar

[31] Mavropoulos E, Rossi A M, da Rocha N C C, Soares G A, Moreira J C and Moure G T 2003 Dissolution of calcium-deficient hydroxyapatite synthesized at different conditions Mater. Charact. 50 203–7.

DOI: 10.1016/s1044-5803(03)00093-7

Google Scholar