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THE MICROPYLAR AREA OF SOME HYMENOPTEROUS EGGS1

Published online by Cambridge University Press:  31 May 2012

J. F. Bronskill  and
E. H. Salkeld
J. F. Bronskill
Affiliation:
Chemistry and Biology Research Institute, Agriculture Canada, Ottawa K1A 0C6
E. H. Salkeld
Affiliation:
Biosystematics Research Institute, Agriculture Canada, Ottawa K1A 0C6
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Extract

During the course of a continuing investigation on egg structure in various insect orders by one of us (EHS), the eggs of some species of Hymenoptera became available for examination with the scanning electron microscope. In this note we report a re-examination of the micropylar region of the egg of the ichneumonid parasitoid Pimpla turionellae (L.) which was originally described by Bronskill (1959), and compare the morphological features of this region with those observed on the same region of the egg of the honey bee, Apis mellifera L. Because of their extremely thin and flexible chorions, the eggs of both species must be freeze-dried to maintain their normal shape in the vacuum of the microscope. They were glued to thin glass coverslips with a dilute solution of Scotch tape - chloroform adhesive, immediately frozen on dry ice, cut in half and freeze-dried for about 16 h at –70°C. The coverslip was mounted on a metal stub with conductive paint and gold-coated just prior to examination in a Cambridge Stereoscan MKI1A.

Type
Articles
Information
The Canadian Entomologist , Volume 110 , Issue 6 , 01 June 1978 , pp. 663 - 665
Copyright
Copyright © Entomological Society of Canada 1978

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References

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