Download PDF
CC BY-NC-ND 4.0 · Avicenna J Med 2017; 07(01): 12-16
DOI: 10.4103/2231-0770.197508
ORIGINAL ARTICLE

Prevalence of TEM, SHV, and CTX-M Beta-Lactamase genes in the urinary isolates of a tertiary care hospital

Trupti Bajpai
Department of Microbiology, Sri Aurobindo Institute of Medical Sciences Medical College and PG Institute, Indore, Madhya Pradesh; Department of Biochemistry, IGNOU, SOS, New Delhi, India
Department of Biochemistry, IGNOU, SOS, New Delhi, India
,
M Pandey
Department of Biochemistry, IGNOU, SOS, New Delhi, India
,
M Varma
Department of Biochemistry, Sri Aurobindo Institute of Medical Sciences Medical College and PG Institute, Indore, Madhya Pradesh, India
,
G S Bhatambare
Department of Microbiology, Sri Aurobindo Institute of Medical Sciences Medical College and PG Institute, Indore, Madhya Pradesh; Department of Biochemistry, IGNOU, SOS, New Delhi, India
› Author Affiliations Financial support and sponsorship Nil.
› Further Information
  PDF Download Permissions and Reprints

Abstract

Introduction: Extended-spectrum beta-lactamases (ESBLs) are the major cause of resistance to beta-lactam antibiotics such as penicillins, cephalosporins, and monobactams. They are derived from the narrow-spectrum beta-lactamases (TEM-1, TEM-2, or SHV-1) by mutations that alter the amino acid configuration around the enzyme active site. Aim: To determine the prevalence of ESBL (bla TEM , bla CTX-M , and bla SHV ) genes among the members of Enterobacteriaceae. Methodology: The present prospective study was carried out from January 2015 to June 2015 in the Department of Microbiology and Molecular Medicine of a Teaching Tertiary Care Hospital. A total of 526 urine samples were studied. Seventy-eight isolates were subjected to polymerase chain reaction for detection of ESBL genes. Results: In our study, ESBL genes were detected among 18 (45%) phenotypically confirmed ESBL producers and 20 (52.5%) phenotypically confirmed non-ESBL producers. The gene that predominated was bla TEM (48.7%), followed by bla CTX-M (7.6%) and bla SHV (5.1%). Conclusion: Definitive identification of ESBL genes is only possible by molecular detection methods. Phenotypic tests need to be evaluated periodically as their performance may change with the introduction of new enzymes.

Keywords

Beta-lactam antibiotics - extended-spectrum beta-lactamases - polymerase chain reaction


Publication History

Article published online:
09 August 2021

© 2017. Syrian American Medical Society. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

Thieme Medical and Scientific Publishers Private Ltd.
A-12, Second Floor, Sector -2, NOIDA -201301, India

 

  • References

  • 1 Sharma J, Sharma M, Ray P. Detection of TEM and SHV genes in Escherichia coli and Klebsiella pneumoniae isolates in a tertiary care hospital from India. Indian J Med Res 2010;132:332-6.
  • 2 Naseer U, Sundsfjord A. The CTX-M conundrum: Dissemination of plasmids and Escherichia coli clones. Microb Drug Resist 2011;17:83-97.
  • 3 Shahid M, Singh A, Sobia F, Rashid M, Malik A, Shukla I, et al. Bla (CTX-M), bla (TEM), and bla (SHV) in Enterobacteriaceae from North-Indian tertiary hospital: High occurrence of combination genes. Asian Pac J Trop Med 2011;4:101-5.
  • 4 Eftekhar F, Rastegar M, Golalipoor M, Mansour Samaei N. Detection of extended spectrum beta-lactamases in urinary isolates of Klebsiella pneumonia in relation to Bla SHV , Bla TEM , Bla CTX-M gene carriage. Iran J Public Health 2012;41:127-32.
  • 5 Collee JG, Fraser AG, Marmian BP, Simmons A, editors. Mackie and McCartney Practical Medical Microbiology. 14 th ed. New York: Churchill Livingstone; 1996. [Reprinted 1999].
  • 6 Cheesbrough M. Microbiology. Medical Laboratory Manual for Tropical Countries. Vol. 2. Cambridgeshire, England: Tropical Health Technology, Norfolk; 1984. p. 985.
  • 7 Thomson KS. Extended-spectrum-β-lactamase, AmpC, and carbapenemase issues. J Clin Microbiol 2010;48:1019-25.
  • 8 Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966;45:493-6.
  • 9 Clinical Laboratory Standards Institute. Performance Standard for Antimicrobial Susceptibility Testing. 24 th Information Supplement. NCCLS Document M100-S23-24. Wayne, Pennsylvania, USA: Clinical Laboratory Standards Institute; 2013-2014.
  • 10 Moosavian M, Deiham B. Distribution of TEM, SHV and CTX-M genes among ESBL-poducing Enterobacteriaceae isolates in Iran. Afr J Microbiol Res 2012;6:5433-9.
  • 11 Ahmed AB, Omar AO, Asghar AH, Elhassan MM. Prevalence of TEM, SHV and CTX-M genes in Escherichia coli and Klebsiella spp. urinary isolates from Sudan with confirmed ESBL phenotype. Life Sci J 2013;10:191-5.
  • 12 Yazdi M, Nazemi A, Mirinargasi M, Jafarpour M, Sharifi SH. Genotypic versus phenotypic methods to detect extended-spectrum beta lactamases (ESBL′s) in uropathogenic Escherichia coli. Ann Biol Res 2012;3:2454-8.
  • 13 Colom K, Pérez J, Alonso R, Fernández-Aranguiz A, Lariño E, Cisterna R. Simple and reliable multiplex PCR assay for detection of blaTEM, bla (SHV) and blaOXA-1 genes in Enterobacteriaceae. FEMS Microbiol Lett 2003;223:147-51.
  • 14 Gangoué-Piéboji J, Bedenic B, Koulla-Shiro S, Randegger C, Adiogo D, Ngassam P, et al. Extended-spectrum-beta-lactamase-producing Enterobacteriaceae in Yaounde, Cameroon. J Clin Microbiol 2005;43:3273-7.
  • 15 Kaftandzieva A, Trajkovska-Dokic E, Panovski N. Prevalence and molecular characterization of ESBLs producind Escherichia coli and Klebsiella pneumonia. Biol Med Sci 2011;32:129-41.
  • 16 Poulou A, Grivakou E, Vrioni G, Koumaki V, Pittaras T, Pournaras S, et al. Modified CLSI extended-spectrum ß-lactamase (ESBL) confirmatory test for phenotypic detection of ESBLs among Enterobacteriaceae producing various ß-lactamases. J Clin Microbiol 2014;52:1483-9.