Research Article

Flow vibration-doubled concentric system coupled with low ratio amine to produce bile acid-macrocapsules of β-cells

Biotechnology & Drug Development Research Laboratory, School of Pharmacy, Curtin Health Innovation Research Institute, Curtin University, Perth, Western Australia, Australia

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Rebecca Negrulj

Biotechnology & Drug Development Research Laboratory, School of Pharmacy, Curtin Health Innovation Research Institute, Curtin University, Perth, Western Australia, Australia

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Hani Al-Salami

*Author for correspondence:

E-mail Address: hani.al-salami@curtin.edu.au

Biotechnology & Drug Development Research Laboratory, School of Pharmacy, Curtin Health Innovation Research Institute, Curtin University, Perth, Western Australia, Australia

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Published Online:19 Feb 2016https://doi.org/10.4155/tde-2015-0001

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Abstract

Background: Pancreatic β-cell microencapsulation using sodium alginate (SA), polylornithine (PLO) copolymers, and ultrasoluble hydrogels, polystyrenes and polyallamines (PAA), has been heavily studied. However, long-term success remains limited due to poor macrocapsules’ physical properties and cell functions. Our study aimed to incorporate percentages of PAA and ursodeoxycholic acid, into SA and PLO dispersion mixture and examine best microencapsulating methods and best macrocapsules containing β-cells. Methods/results: Microencapsulating parameters were examined and the Flow-Vibrational Nozzle built-in system was screened and found to be most efficient at high frequency (1900 Hz). Macrocapsules were produced with or without ursodeoxycholic acid in percentages: 0.018SA:0.01PLO:0.005PAA:0.04ursodeoxycholic acid (up to 100% H₂O). Using the refined microencapsulation method with vibrational frequency of 1900 Hz, macrocapsules with ursodeoxycholic acid had optimized cell viability and biological functions and ameliorated inflammatory biomarkers. Conclusion: High frequency and air-pressure with Flow-Vibrational encapsulation using the mixture: 0.018SA:0.01PLO:0.005PAA:0.04ursodeoxycholic acid resulted in better cell biology suggesting potentials in β-cell transplantation.

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References

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Vol. 7, No. 3

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Published online 19 February 2016

Published in print March 2016

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Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.

Acknowledgements

The authors acknowledge the Curtin Health Innovation Research Institute for provision of laboratory space and technology platforms utilized in this study. The authors also acknowledge the use of laboratory equipment, scientific and technical assistance of the Microscopy and Microanalysis Facility (MMF) at Curtin University, which has been partially funded by the University, State and Commonwealth Governments. The authors acknowledge the ARC Centre of Excellence in Plant Energy Biology (University of Western Australia) for training, support and access to equipment. The NIT-1 cells were a generous donation from Professor Grant Morahan at the University of Western Australia.

Financial & competing interests disclosure

The authors acknowledge the Australian Postgraduate Award (APA) & Curtin Research Scholarship (CRS). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

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