The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Taxonomical Classification and Species-specific Detection of Genus Some Phellinus using Phylotype

Phylotype에 의한 수종의 Phellinus속의 분류체계 확립 및 종간구별을 위한 신속동정법 개발

  • Kim, Cheng-Yun (Department of microbiology, College of Natural Sciences, Pusan National University) ;
  • Lee, Jae-Yun (Department of microbiology, College of Natural Sciences, Pusan National University) ;
  • Kim, Gi-Young (Department of microbiology, College of Natural Sciences, Pusan National University) ;
  • Lee, Ki-Won (Department of microbiology, College of Natural Sciences, Pusan National University) ;
  • Park, Jae-Min (Department of microbiology, College of Natural Sciences, Pusan National University) ;
  • Kim, Mun-Ok (Department of microbiology, College of Natural Sciences, Pusan National University) ;
  • Lee, Tae-Ho (Department of microbiology, College of Natural Sciences, Pusan National University) ;
  • Lee, Jae-Dong (Department of microbiology, College of Natural Sciences, Pusan National University)
  • 김성윤 (부산대학교 자연과학대학 미생물학과) ;
  • 이재윤 (부산대학교 자연과학대학 미생물학과) ;
  • 김기영 (부산대학교 자연과학대학 미생물학과) ;
  • 이기원 (부산대학교 자연과학대학 미생물학과) ;
  • 박재민 (부산대학교 자연과학대학 미생물학과) ;
  • 김문옥 (부산대학교 자연과학대학 미생물학과) ;
  • 이태호 (부산대학교 자연과학대학 미생물학과) ;
  • 이재동 (부산대학교 자연과학대학 미생물학과)
  • Published : 2003.12.30
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Abstract

This study was carried out to identify the phylogenetic relationship of Phellinus species and to know its distribution by comparing the DNA sequences of internal transcribed spacer regions(ITS1 and IST2) and 5.8S ribosomal DNA (rDNA) repeat unit. The Phellinus species had their specific sequences in IST1 and 2 regions depending on suedes. The comparison of the ITS sequences of standard strains indicated that the sequences of ITS1 were more variable than those of ITS2. Nine strains of the commercial products of Phellinus species used in this study were identified as P. lintues, P. baumii, P. igniarius, and P. pini. Most of commercial species were P. pini and P. baumii, and P. gilvus was not found. Also, P. linteus was only found in form of mycelial culture rather than fruiting body. Moreover, the species-specific primers were designed based on ITS sequence data. Each species-specific primers were bound in P. lintues(ITSF-PL2R), P. baumii(PB1F-ITS4R), P. igniarius(IF1-IR3), P. pini(PF1-PR3), and P. gilvus(GF2-GR4), respectively. These primer sets would be useful fer the detection of specific-species among unidentified Phellinus species rapidly.

국내 유통균주에 대한 Phellinus속의 분류체계를 확립하고 종간구별을 위한 신속동정법을 개발하기 위해 계통학적 정보를 지니고 있는 ITS1, 5.8S rRNA및 ITS2부위의 염기서열을 밝히고 ITS1과 ITS2 부위의 다양한 염기서열을 이용하여 종 특이적인 유전자 탐침을 개발하였다. 본 연구에서 조사된 바에 의하면, 상황버섯으로 시판되고 있는 국내유통균주는 총 9균주로서 P. pini가 4종, P. baumii가 3종, P. igniarius와 P. linteus가 각각 1종으로 확인되었으며 P. gilvus은 조사되지 알았다. 그 중 P. pini는 대부분 중국에서 수입된 제품이었고 P. igniarius를 포함한 P. baumii는 주로 북한산 제품으로 조사되었다. 한편 P. linteus와 P. baumii는 변이가 높은 ITS1 , ITS2 부위에서도 다른 종에 비하여 높은 상동성을 나타내어 종 특이적인 유전자 탐침이 유효하지 않은 반면에 P. igniarius, P. pini, P. gilvus종에 대한 detection primer로 각각 IF1-IR3, PF1-PF3, GF2-FR4 primer는 유전자 탐침이 유효함을 확인하였다. 유연관계가 아주 가까운 P. linteus와 P. baumii에 대한 정확한 분석을 위해서는 변이가 심한 ITS 부위와 보존성이 높은 18S rDNA 부위의 염기서열을 함께 분석, 비교하여야 가능하리라 사료된다.

Keywords

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