The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Maintenance of Filamentous Fungi in Korean Agricultural Culture Collection (KACC)

농업미생물은행(KACC)의 곰팡이 보존

  • Shin, Myeong-Suk (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Hong, Seung-Beom (Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration)
  • 신명숙 (국립농업과학원 농업미생물과) ;
  • 홍승범 (국립농업과학원 농업미생물과)
  • Received : 2014.06.27
  • Accepted : 2014.06.30
  • Published : 2014.06.30
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Abstract

A total of 7039 strains of filamentous fungi are preserved in Korean Agricultural Culture Collection (KACC). The 4065 strains (58%) of them, which produce many spores in cultivation on proper media, are preserved with freeze-drying method. They are also preserved with liquid nitrogen and deep-freezer storage in order to minimize loss by death. Aspergillus, Penicillium, Lichtheimia, Mucor, Rhizopus, etc. which are common in surrounding environments, are included in this category. The others which do not produce spores, or produce few spores in vitro, are preserved with liquid nitrogen, deep-freezer and mineral oil storage. Phytophthora, Pythium, Cercospora, Septoria, Rhizoctonia, etc. are included in this category. The authors also introduced various fungal preservation methods and provided detailed preservation procedures that are used in KACC.

농업미생물은행(KACC)에는 7,039균주의 곰팡이가 장기 보존되어 있다. 이 중에서 다수의 포자를 형성하는 4,065균주는 동결건조법으로 보존되어 있는데 사멸로 인한 균주의 소실을 막기 위하여 대부분은 액체질소보존법 그리고 모든 균주가 저온냉동고보존법으로 함께 보존되어 있다. 4,065균주의 곰팡이에는 주로 공기 중에 흔하며 산업적으로 이용성이 많은 Aspergillus, Penicillium, Lichtheimia, Mucor, Rhizopus 속 등이 포함된다. 포자를 형성하지 않거나 소수형성하거나 또한 보존이 어려운 너무 큰 포자를 형성하는 나머지 곰팡이는 동결건조보존법으로 보존이 불가하기 때문에 액체질소보존법, 저온냉동고보존법 그리고 광유보존법으로 균주를 보존한다. 여기에는 Phytophthora, Pythium, Cercospora, Septoria, Rhizoctonia 속 등의 식물병원균이 포함된다. 이 외에도 KACC에서 이용하는 다양한 곰팡이 보존법을 소개하고 이들의 상세보존 과정을 기술한다.

Keywords

References

  1. Jong SC, Birmingham JM. Cultivation and preservation of fungi in culture. In: The Mycota VII, Systematics and evolution, eds. McLaughlin DJ, McLaughlin EG, Lemke PA. pp. 193-202. Springer-Verlag Berlin Heidelerg; 2001.
  2. Kirsop BE, Doyle A. Maintenance of microorganisms and cultured cells - a manual of laboratory methods, 2nd ed. Academic Press; 1991.
  3. Smith D, Onions AHS. The preservation and maintenance of living fungi. CAB International Wallingford. UK; 1994.
  4. Web site of CABRI Guideline [Internet], [Cited 25 June 2014]. Available from http://www.cabri.org/guidelines/microorganisms/MCover1.html.
  5. Bae KS. Long term storage of microorganisms - Focusing on yeast and mold. Special issue for 2011 Spring Congress of Microbial Society of Korea 1991;4:38-48.
  6. Lee JK, Choi KJ, Kim BS, Cho KY. Storage of phytopathogenic fungal cultures in sterile distilled water. Kor J Plant Pathol 1994;10:144-7.
  7. Korean Society of Plant Pathology. Long term storage of plant pathogenic germplasm. Korean Society of Plant Pathology. Suwon; 1995.
  8. Korean Agricultural Culture Collection. Korean translation of 'CABRI guideline-microorganisms'. National Institute of Agricultural Biotechnology, Suwon; 1993.
  9. Korean Agricultural Culture Collection. Laboratory procedures for maintenance of microorganism in KACC. National Institute of Agricultural Biotechnology, Suwon; 1995.
  10. Jeon YA, Shin MS, Kim HJ, Kim DH, Go SJ, Hong SB. Preservation of fungi in liquid nitrogen using polypropylene straws. Kor J Mycol 2006;34:54-8. https://doi.org/10.4489/KJM.2006.34.1.054
  11. Korean Agricultural Culture Collection. Laboratory procedures for maintenance of microorganism in KACC. 2nd ed. National Academy of Agriculutural Science, Suwon; 2010.
  12. Hong SB. Preservation of mushroom strains. In: Mushroom science, pp. 266-85. Eds. Yoo YB, Koo CD, Kim SH, Seo GS, Shin HD, Lee JW, Lee CS, Chang HY. Nature & Human, Seoul; 2010.

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