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Silver nanoparticles have particular properties that contribute to their very promising applications, novel in various fields of science, such as the development of biosensors, the diagnosis and treatment of cancer, the controlled release of drugs and the antimicrobial potential. The biological synthesis of nanoparticles is of great interest over other physical and chemical methods because the use of toxic chemicals and drastic reaction conditions are avoided. The extracellular biosynthesis using fungi could also make downstream processing much easier than the intracellular biosynthesis. One of the main applications of silver nanoparticles is their antimicrobial activity. Several studies have demonstrated the bactericidal properties of silver nanoparticles are different from silver ions, and that they are strongly influenced by their shape, size, concentration and colloidal state. In the present work, the ability of fungal strains from Uruguay to synthesize silver nanoparticles was studied. Eight fungi were able to synthesize nanoparticles. An extensive physicochemical characterization of the nanoparticles was carried out including ultraviolet-visible spectroscopy, transmission electron microscopy, dynamic light scattering, zeta-potential and gel electrophoretic mobility. According to the characterization and colloidal stability results, nanoparticles from three fungi were selected for antimicrobial activity assays. All nanoparticles were able to inhibit Escherichia coli growth, demonstrating their potential as effective antibacterial agent for use in biomedical applications.


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Extracellular Biosynthesis of Silver Nanoparticles Using Fungi and Their Antibacterial Activity

Show Author's information Paula Sanguiñedo1Raluca María Fratila2,3María Belén Estevez1Jesús Martínez de la Fuente2,3Valeria Grazú2,3( )Silvana Alborés1( )
Área de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay
Instituto de Ciencia de Materiales de Aragón, CSIC, Zaragoza, España
Centro de Investigación Biomédica en red en Bioingenieria Biomateriales y Nanomedicina (CIBER-BBN), Zaragoza, Spain

Abstract

Silver nanoparticles have particular properties that contribute to their very promising applications, novel in various fields of science, such as the development of biosensors, the diagnosis and treatment of cancer, the controlled release of drugs and the antimicrobial potential. The biological synthesis of nanoparticles is of great interest over other physical and chemical methods because the use of toxic chemicals and drastic reaction conditions are avoided. The extracellular biosynthesis using fungi could also make downstream processing much easier than the intracellular biosynthesis. One of the main applications of silver nanoparticles is their antimicrobial activity. Several studies have demonstrated the bactericidal properties of silver nanoparticles are different from silver ions, and that they are strongly influenced by their shape, size, concentration and colloidal state. In the present work, the ability of fungal strains from Uruguay to synthesize silver nanoparticles was studied. Eight fungi were able to synthesize nanoparticles. An extensive physicochemical characterization of the nanoparticles was carried out including ultraviolet-visible spectroscopy, transmission electron microscopy, dynamic light scattering, zeta-potential and gel electrophoretic mobility. According to the characterization and colloidal stability results, nanoparticles from three fungi were selected for antimicrobial activity assays. All nanoparticles were able to inhibit Escherichia coli growth, demonstrating their potential as effective antibacterial agent for use in biomedical applications.

Keywords: Antibacterial activity, Silver nanoparticles, Biosynthesis, Fungi

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Publication history
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Publication history

Received: 12 January 2018
Accepted: 22 May 2018
Published: 01 June 2018
Issue date: June 2018

Copyright

© Paula Sanguiñedo, Raluca María Fratila, María Belén Estevez, Jesús Martínez de la Fuente, Valeria Grazú, and Silvana Alborés.

Acknowledgements

This work was supported by the Comisión Sectorial de Investigación Científica (Universidad de la República, Uruguay), Program for the Development of Basic Sciences (PEDECIBA-Química) and Agencia Nacional de Investigación e Innovación (ANII), Uruguay. R. M. F. acknowledges financial support from MINECO (Ramón y Cajal grant RYC-2015-17640). The microscopy works were conducted in the Laboratorio de Microscopias Avanzadas, Instituto de Nanociencia de Aragon-Universidad de Zaragoza. Authors acknowledge the LMA-INA for offering access to their instruments and expertise.

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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