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＜departmental bulletin paper＞
Optimum Conditions for the Assay of the Classical Pathway-Complement Titer of Tilapia (Tilupia nilotica) Serum

Creator
Author PID
K001531
Creator Name
Yano, Tomoki
矢野, 友紀
Affiliation
Affiliation Name
Laboratory of Fisheries Chemistry, Faculty of Agriculture, Kyushu University
九州大学農学部水産学科水産化学第一講座
Creator Name
Hatayama, Yukihiro
Affiliation
Affiliation Name
Laboratory of Fisheries Chemistry, Faculty of Agriculture, Kyushu University
Creator Name
Matsuyama, Hiroko
松山, 博子
Affiliation
Affiliation Name
Laboratory of Fisheries Chemistry, Faculty of Agriculture, Kyushu University
九州大学農学部水産学科水産化学第一講座
Author PID
K001611
Creator Name
Nakao, Miki
中尾, 実樹
Affiliation
Affiliation Name
Laboratory of Fisheries Chemistry, Faculty of Agriculture, Kyushu University
九州大学農学部水産学科水産化学第一講座
Language
English
Publisher
Kyushu University
九州大学農学部
Date
1988-10
Source Title
Journal of the Faculty of Agriculture, Kyushu University
Vol
33
Issue
1/2
First Page
29
Last Page
36
Publication Type
Version of Record
Access Rights
open access
Crossref DOI
https://doi.org/10.5109/23914
Related DOI
九州大学農学部紀要 || 33(1/2) || p29-36
Journal of the Faculty of Agriculture, Kyushu University || 33(1/2) || p29-36
http://www.agr.kyushu-u.ac.jp/
Related URI
九州大学農学部紀要 || 33(1/2) || p29-36
Journal of the Faculty of Agriculture, Kyushu University || 33(1/2) || p29-36
http://www.agr.kyushu-u.ac.jp/
Relation
九州大学農学部紀要 || 33(1/2) || p29-36
Journal of the Faculty of Agriculture, Kyushu University || 33(1/2) || p29-36
http://www.agr.kyushu-u.ac.jp/

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Details

PISSN
0023-6152
NCID
AA00697606
Record ID
23914
Peer-Reviewed
Unrefereed
Subject Terms
Sheep red blood cells (SRBC)
Tilapia serum
Classical pathway-complement titer (CH50)
ISSN
0023-6152
NCID
AA00697606
Notes
The optimum conditions for the assay of the classical pathway-complement titer (CH50) of tilapia serum were investigated by use of sheep red blood cells (SRBC) sensitized with tilapia antibody. The antiserum was obtained from tilapia immunized with SRBC stroma for 35 days, and heat-treated at 46℃ for 20min to inactivate the complement coexisting in the serum. The optimum conditions for the hemolytic complement reaction were found to be as follows : reaction temperature, 25℃ ; pH, 7.5-8.0 ; reaction period, 120 min ; concentrations of Ca²⁺ and Mg²⁺, 1.0 mM and 1.0 mM, respectively. Under these conditions, the CH50 of 15 tilapia specimens (240-960 g) which were obtained from a fish farm in Fukuoka City from September in 1987 to January in 1988 were assayed. The titers ranged from 153 to 392 CH50 units/ml, mean±SD being 250170 CH50 units/ml. These CH50 values of tilapia sera were extremely high as compared with those reported for other bony fishes.
Created Date 2012.08.01
Modified Date 2022.11.11

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