ICJEM
Intercontinental Journal of Emergency Medicine

The Intercontinental Journal of Emergency Medicine aims to publish issues related to all fields of emergency medicine and all specialties involved in the management of emergencies in the hospital and prehospital environment of the highest scientific and clinical value at an international level and accepts articles on these topics. This journal is indexed by indices that are considered international scientific journal indices (DRJI, ESJI, OAJI, etc.). According to the current Associate Professorship criteria, it is within the scope of International Article 1-d. Each article published in this journal corresponds to 5 points.

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Original Article
Investigation of changes in MDH enzyme activity in epilepsy
Abstract
Aims: Epilepsy is one of the most common neurological diseases. It is classified into three groups: focal onset, generalized onset and unclassifiable. A seizure type is difficult to determine. The malate dehydrogenase (MDH) enzyme has a critical role in the excitability of the brain, and it has been reported that recurrent seizures are seen when its regulation is disturbed. We investigated the contribution of MDH enzyme levels as a biomarker to seizure classification.
Methods: Our study was conducted prospectively by patients who were admitted to the emergency department of our hospital within a six-month period. Included in the study were 65 patients diagnosed with epilepsy, according to the International League Against Epilepsy (ILAE) classification. The patients were divided into two groups according to seizure type: focal onset and generalized onset. They were compared in terms of enzyme activity.
Results: The MDH values of the patients with primary epilepsy were found to be 105.94±111.43 and those with secondary epilepsy were 81.03±121.59 (p=0.141). The same test was used to compare the distribution of MDH values between seizure type groups. The MDH value was 47.56±38.65 in patients with focal-onset epilepsy and 109.76±128.44 in patients with generalizedonset epilepsy. A statistically significant difference was observed in the comparisons between the groups (p= 0.031)
Conclusion: Our results reveal the potential of MDH as a biomarker that can be used in epilepsy. In addition, the statistically significant difference between focal-onset and generalized-onset epilepsy indicates that it can be a usable biomarker in seizure classification. MDH enzyme level has a cut-off value of 109.76±128.44 IU/L strongly underlines that it should be used in the diagnosis.

Keywords : Epilepsy, malate dehydrogenase, generalized-onset

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1. Fiest KM, Sauro KM, Wiebe S, et al. Prevalence and incidence ofepilepsy: a systematic review and meta-analysis of international studies.Neurology. 2017;88(3):296-03.
2. Shorvon S, Walker M. Status epilepticus in idiopathic generalizedepilepsy. Epilepsia. 2005;46(9):73-9
3. Falco-Walter JJ, Scheffer IE, Fisher RS. The new definition andclassification of seizures and epilepsy. Epilepsy Res. 2018;139:73-9
4. Fisher RS, Cross JH, French JA, et al. Operational classificationofseizure types by the International League Against Epilepsy: PositionPaper of the ILAE Commission for Classification and Terminology.Epilepsia. 2017;58:522-30
5. Menon B, Ramalingam K, Kumar RV. Oxidative stress in patients withepilepsy is independent of antiepileptic drugs. Seizure. 2012;21(10):780-4
6. Nemade ST, Melinkeri RR. Effect of antiepileptic drugs on antioxidantstatus in epilepsy. Curr Neurobiol. 2010;1(2):109-12
7. Menon B, Ramalingam K, Kumar RV. Oxidative stress in patients with epilepsy is independent of antiepileptic drugs. Seizure. 2012;21(10):780-4
8. Shi Q, Gibson GE. Up-regulation of the mitochondrial malatedehydrogenase by oxidative stress is mediated by miR-743a. JNeurochem. 2011;118(3):440-8
9. Laur D, Dozieres-Puyravel B, Ilea A, et al. Focal epilepsy due to de novoSCN1A mutation. Epileptic Disord. 2021;23(3):459-65
10. Benn EK, Hauser WA, Shih T, et al. Estimating the incidence of firstunprovoked seizure and newly diagnosed epilepsy in the low-incomeurban community of Northern Manhattan, New York City. Epilepsia.2008;49(8):1431-9
11. Hesdorffer DC, Tomson T, Benn E, et al. ILAE Commission onEpidemiology; Subcommission on Mortality. Combined analysis of riskfactors for SUDEP. Epilepsia. 2011;52(6):1150-9
12. Muralidhar V, Venugopal K. New onset seizures: Etiology and co-relation of clinical features with computerized tomography andelectroencephalography. J Sci Soc. 2015;42(2):82-87.
13. Chalasani S, Kumar MR. Clinical profile and etiological evaluationof new onset seizures after age 20 years. IOSR J Dent Med Sci.2015;14(2):2279-2861.
14. Kanitkar SA, Gaikwad AN, Kalyan M, et al. Study of seizure disorderin elderly: Etiology, types, EEG and image findings. Transworld Med J.2013;1:24-5
15. Steinlein OK. Genes and mutations in idiopathic epilepsy. Am J MedGenet. 2001;106(2):139-45
16. Beghi E. The Epidemiology of Epilepsy. Neuroepidemiology.2020;54(2):185-91
17. Aaberg KM, Suren P, Søraas CL, et al. Seizures, syndromes, andetiologies in childhood epilepsy: The International League AgainstEpilepsy 1981, 1989, and 2017 classifications used in a population-basedcohort. Epilepsia. 2017;58(11):1880-1891.
18. Alexandre V Jr, Capovilla G, Fattore C, et al. Characteristics of a largepopulation of patients with refractory epilepsy attending tertiaryreferral centers in Italy. Epilepsia. 2010;51(5):921-925.
19. Hirani MM, Shrivastva S. Clinical profile of new onset seizures inadults. Indian J Appl Res 2015;5:19-21.
20. Galizia EC, Faulkner HJ. Seizures and epilepsy in the acutemedical setting: presentation and management. Clin Med (Lond).2018;18(5):409-13.
21. Lerche H, Jurkat-Rott K, Lehmann-Horn F. Ion channels and epilepsy.Am J Med Genet. 2001;106(2):146-59.
22. Xu X, Guo F, Lv X, et al. Abnormal changes in voltage-gated sodiumchannels Na(V)1.1, Na(V)1.2, Na(V)1.3, Na(V)1.6 and in calmodulin/calmodulin-dependent protein kinase II, within the brains ofspontaneously epileptic rats and tremor rats. Brain Res Bull. 2013;96:1-9
23. Ait-El-Mkadem S, Dayem-Quere M, Gusic M, et al. Mutations inMDH2, encoding a krebs cycle enzyme, cause early-onset severeencephalopathy. Am J Hum Genet. 2017;100(1):151-9.
24. Selek S, Gul AZ, Atakul N, Meydan S, Sarikaya A, Koktasoglu F, et al.Investigation of serum isocitrate dehydrogenase, malate dehydrogenaseand glutamate dehydrogenase activities with related oxidative stressmarkers in preeclampsia. Authorea. 2021;3:1-2.
25. Cheng C, Sirven JI, Ryan DJ, Feyissa AM. Looks can be deceptive: Aprimary generalized epilepsy mimicking a lesional focal-reflex epilepsy.Seizure. 2021;10(91):114-6.
Article available in :
Volume 1, Issue 1, 2023
Page : 1-3
https://doi.org/10.51271/ICJEM-0001
Article Information
Received : 15 Mar 2023
Accepted : 27 Mar 2023
Published : 30 Mar 2023
Corresponding Author :

Bahadır Taşlıdere: Department of Emergency Medicine, Faculty of Medicine, Bezmialem Vakif University, Istanbul, Turkey

[email protected]
Citation : Taşlıdere B, Güven EC, Oflaz Güven B, Gülen B. Investigation of changes in MDH enzyme activity in epilepsy. Intercont J Emerg Med. 2023;1(1):1-3.

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