Complex Image Analysis Tasks: What, How, and Who

     As microscopy diversifies and becomes more complex, the problem of quantification of microscopy images has emerged as a major roadblock for many researchers. There are challenges that all researchers face, independent of their scientific question and the images they've generated. Complexities may arise at many stages throughout the analysis process, including handling of the source data, image pre-processing, object finding, or measurement, and statistical analysis. While the exact solution required for each complexity will be problem-specific, by understanding tools and tradeoffs, optimizing data quality, breaking workflows and data sets into chunks, talking to experts, and thoroughly documenting what has been done, analysts at any experience level can learn to overcome these challenges and create better and easier image analyses.