Enzymatic Synthesis of Nonreducing Trisaccharide Containing Both Lactose and Trehalose as a Part of the Structure

Tetsuya Ishii; Morimasa Tanimoto; Yasuharu Itagaki; Yoshihiko Honda; Than Than Win; Chihiro Kawase; Ayano Kita; Syuichi Onodera; Hiroyuki Ito; Hirokazu Matsui; Mamoru Honma

doi:10.5458/jag.47.193

Tetsuya Ishii, Morimasa Tanimoto, Yasuharu Itagaki, Yoshihiko Honda, Than Than Win, Chihiro Kawase, Ayano Kita, Syuichi Onodera, Hiroyuki Ito, Hirokazu Matsui, Mamoru Honma

Author information

Tetsuya Ishii
Snow Brand Milk Products Co., Ltd., Sapporo Research Laboratory
Morimasa Tanimoto
Snow Brand Milk Products Co., Ltd., Sapporo Research Laboratory
Yasuharu Itagaki
Snow Brand Milk Products Co., Ltd., Sapporo Research Laboratory
Yoshihiko Honda
Snow Brand Milk Products Co., Ltd., Sapporo Research Laboratory
Than Than Win
Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
Chihiro Kawase
Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
Ayano Kita
Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
Syuichi Onodera
Department of Food Science, Faculty of Dairy Science, Rakuno Gakuen University
Hiroyuki Ito
Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
Hirokazu Matsui
Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
Mamoru Honma
Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University

JOURNAL FREE ACCESS

2000 Volume 47 Issue 2 Pages 193-196

DOI https://doi.org/10.5458/jag.47.193

Details

Abstract

To synthesize nonreducing oligosaccharide derived from trehalose, enzymatic transgalactosylation was investigated using lactose and trehalose as substrates and a commercially available β-ga-lactosidase, which has a tendency to transfer the galactosyl residue to the C4 position of acceptor sugars, from Bacillus circulans. The galactosylation successfully resulted in the efficient production of O-β-D-galactopyranosyl-(1-4)-α, α-trehalose. This trisaccharide showed good stability against acidic treatment.

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