Abstract
Hairy roots of Lactuca virosa L. transformed with Agrobacterium rhizogenes and treated with methyl jasmonate, are capable of producing sesquiterpene lactones - crepidiaside B and lactuside A in amounts higher than in untreated roots. An HPLC analysis showed the highest content of crepidiaside B after 24 hours of methyl jasmonate treatment (60% above the control). The increase in the content of lactuside A was relatively small and reached 27% after 48 hours of the treatment, in comparison with the control.
Keywords
Lactuca virosa L.; Asteraceae; hairy root culture; Agrobacterium rhizogenes; methyl jasmonate; sesquiterpene lactones; lactuside A; crepidiaside B