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Title: CRISPR/CAS IN GENOMES OF SINORHIZOBIUM MELILOTI
CRISPR/CAS IN GENOMES OF SINORHIZOBIUM MELILOTI

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M.Vladimirova;V. Muntyan;A. Kozlova;M. Grudinin;M. Roumiantseva
10.5593/sgem2020/6.1/s25.028
1314-2704
978-619-7603-12-5
English
20
6.1
Advances in Biotechnology
Currently, CRISPR/Cas
systems are actively studied bacterial systems, due to the
possibilitpossibility to apply them for targeted editing of eukaryotic genomes. CRISPR/Cas
systems are belonged to adaptive immun immune system of bacteria and archaea acting against
invading mobile genetic elements (MGEs: bacteriophages and integrative conjugative
elements ). Curre Currently CRISPR/Cas systems were detected in genomes of 75% of known
archaea and 36 % of bacteria strains . However, there are still quite less knowledge about
CRISPR/Cas systems in genomes of nitrogen nitrogen-fixing nodule bacteria.
Sinorhizobium meliloti
are agricultu agriculturally valuable species of soil bacteria that form
nitrogennitrogen-fixing symbiosis with alfalfa cross cross-inoculation group. Evaluation of adaptive
immunimmune systems protecting root nodule bacteria against external foreign DNA is actual;
since S. meliloti is actively in involved in horizontal gene transfer occurred in rhizosphere
and soil microbiome where lysogenic phages and conjugative mobile elements are
abund ant . The aim of this work was to search and study diversity of CRISPR/Cas in S.
melilotimeliloti.
CRISPR/Cas sequences
we re identified by b ioinformatics analysis of full -genome
sequenced of 22 S. meliloti strains posted in GenBank. The prediction of potential
CRISPR/Cas sequences was done by using CRISPRCas++ CRISPRCas++, genomic islands (GIs) and
prophages (Phs) were identified by appl applying Islander algorithm and PHASTER,
respectively. For the first time we are reporting here about potential CRISPR sequences
(hereinafter CRISPR sequences ) in S. meliloti genomes. The number of sequences of
interest varied from 3 to 9 among tested strain strains. Besides that sequences encoding Cas3 a
potential element of CRISPR/Cas system of Type I were identified on chromosome and
megaplasmid SMb. Some sequences a mong identified CRISPR were unique unique, while others were common for all tested genetically distant stra strains. CRISPR spacer sequences
identified in S. meliloti are most mostly similar to fragments of phages from Siphoviridae and
Podoviridae familiesfamilies.
Summarizing, CRISPR sequences in
S. meliloti are located not only on chromosome,
but on megaplasmids and on cryptic plasmids as well. These elements of adaptive
immunity systems of S. meliloti aimed to protect bacteria cells from bacteriophages
mainly from Siphoviridae and Podoviridae families widely ab abou nd ed in soil
microbiomes. This work is supported by the RSF 20 20-16 -00105.
conference
https://www.sgem.org/index.php/jresearch-article?citekey=Vladimirova202025215222
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20th International Multidisciplinary Scientific GeoConference SGEM 2020
20th International Multidisciplinary Scientific GeoConference SGEM 2020, 18 - 24 August, 2020
Proceedings Paper
STEF92 Technology
International Multidisciplinary Scientific GeoConference-SGEM
SWS Scholarly Society; Acad Sci Czech Republ; Latvian Acad Sci; Polish Acad Sci; Russian Acad Sci; Serbian Acad Sci & Arts; Natl Acad Sci Ukraine; Natl Acad Sci Armenia; Sci Council Japan; European Acad Sci, Arts & Letters; Acad Fine Arts Zagreb Croatia; C
215-222
18 - 24 August, 2020
website
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7536
CRISPR/Cas; Sinorhizobium meliloti; genomic islands; bacteriophages