Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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Structure and Characteristics of Bacterial Community on Biofilm of Food Wastewater Treatment System in Winter

식품폐수 처리 공정용 생물막의 겨울철 세균군집 구조와 특성

  • Lee, Dong-Geun (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Yoo, Ki-Hwan (Biotechnology Research Division, National Fisheries Research & Development Institute) ;
  • Park, Seong-Joo (Department of Biomedical Laboratory Science, Daejeon University)
  • 이동근 (신라대학교 의생명과학대학 제약공학과) ;
  • 유기환 (국립수산과학원 생명공학연구단) ;
  • 박성주 (대전대학교 임상병리학과)
  • Received : 2011.01.25
  • Accepted : 2011.03.22
  • Published : 2011.04.30
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Abstract

Biofilm and aeration tank of pilot and full RABC (rotating activated Bacillus contactor) plant were analyzed to characterize and determine bacterial community structure in food wastewater treatment system at winter. Concentration of heterotrophic bacteria and Bacillus group was $10^7$ and $10^5$ CFU/ml, respectively, at biofilm of pilot-plant while others represented $10^6$ and $10^4$ CFU/ml, respectively. Five and eight phyla were detected at biofilm of pilot- and full-plant, respectively, by 16S rDNA sequencing. Biofilm of pilot-plant was dominated by ${\beta}$-Proteobacteria (38.8%), ${\gamma}$-Proteobacteria (22.4%), and Bacteroidetes (12.2%), and the most dominant genus was Zoogloeae genus (22.4%). Candidate division TM7 (12.5%) was only detected at biofilm of full-plant and it was dominated by Bacteroidetes (33.3%), ${\gamma}$-Proteobacteria (29.2%), and ${\beta}$-Proteobacteria (20.8%). Clostridium genus specific primer set enabled to detect the sequences of Clostridium genus. These suggested that anaerobic and aerobic bacteria were coexisted even from the initial period of biofilm formation and ${\beta}$-Proteobacteria, ${\gamma}$-Proteobacteria and Bacteroidetes were major phyla in biofilm of food wastewater treatment system at winter.

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