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Submitted
October 14, 2022
Accepted
December 1, 2022
Published
December 29, 2022
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Abstract

 


Background: Global proliferation of the pharmaceutical industry in response to devastating health challenges has led to several ecological and environmental problems. Heavy metal contamination is one of the most dangerous factors that affect ecosystems and human health. This study aimed to detect the presence of heavy-metal resistance genes in clinical and environmental sources using polymerase chain reaction (PCR).


Method: This study employed an experimental design. The samples included 40 clinical isolates (blood, urine, throat, wound, and sputum) and 40 environmental isolates (pharmaceutical effluents).The isolates were collected and subcultured in nutrient broth for analysis.


Results: The result shows that heavy metal-resistant genes were not detected in the clinical isolates whereas 65% and 60% of the environmental isolates harbored silver (Ag) and arsenic (As) respectively. However, the clinical isolates in this study showed higher rates of resistance than the environmental isolates did. This may be due to the frequent use of broad-spectrum antibiotics during clinical sessions, leading to the development of antibiotic-resistant strains.


Conclusion: Heavy metal-resistant genes are present in bacteria in the environment and may cause severe health issues (drug resistance) when these genes are transmitted to pathogenic organisms that affect humans and animals. The association between bacterial drug resistance and heavy metal acquisition may greatly influence treatment outcomes in the event of infection. Therefore, control measures should be implemented to reduce the dissemination of these metals in the environment.

Keywords

Antibiotics heavy mental-resistant genes Pharmaceutical effulent bateria

Article Details

How to Cite
Sendolo, D., Baysah, G., & Onyebuchi Ezeamagu, C. . (2022). Detection of Heavy Metal-Resistance Gene in Bacteria Isolated from Clinical and Environmental Sources Using Polymerase Chain Reaction (PCR). Pan-African Journal of Health and Environmental Science, 1(2), 83–92. https://doi.org/10.56893/ajhes.2022-v1i2.239
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References

  1. Adewole M.B. &Uchegbu L. U. (2010). Properties of soils and plants uptake within the vicinity of selected automobile workshops in Ile-Ife Southwestern Nigeria. Ethiopian Journal of Environmental Studies and Management, 3(3), 31-35. https//doi.org/10.4314/ejesm.v3i3.63962
  2. Altug, G., & Balkis, N. (2009). Levels of some toxic elements and frequency of bacterial heavy metal resistance in sediment and sea water. Environmental monitoring and assessment, 149(1-4), 61–69. https://doi.org/10.1007/s10661-008-0183-z
  3. Baker-Austin, C., Wright, M. S., Stepanauskas, R., & McArthur, J. V. (2006). Co-selection of antibiotic and metal resistance. Trends in microbiology,14(4), 176–182. https://doi.org/10.1016/j.tim.2006.02.006
  4. Baquero F. (2001). Low-level antibacterial resistance: A gateway to clinical resistance. Drug Resistance Updates Reviews And Commentaries In Antimicrobial And Anticancer Chemotherapy, 4(2), 93–105. https://doi.org/10.1054/drup.2001.0196
  5. Cesare, A.D., Eckert, E., & Corno, G. (2016). Co-selection of antibiotic and heavy metal resistance in freshwater bacteria. J Limnol,75,1198.
  6. Ezeamagu, C. O., Dada, O. G., Omohoro, M.U., & Mokoshe, W.N. (2020). Evaluation of resistance determinants in gram-negative bacteria obtained from fish ponds and animal-based wastes in South-West, Nigeria. Acta SATECH, 12 (2): 23 – 36) 12 (2), 23-36.
  7. Fang, L., Li, X., Li, L., Li, S., Liao, X., Sun, J., & Liu, Y. (2016). Co-spread of metal and antibiotic resistance within ST3-IncHI2 plasmids from E. coli isolates of food-producing animals. Scientific Reports, 6, 25312. https://doi.org/10.1038/srep25312
  8. Fard, R. M., Heuzenroeder, M. W., & Barton, M. D. (2011). Antimicrobial and heavy metal resistance in commensal enterococci isolated from pigs. Veterinary Microbiology, 148(2-4), 276–282. https://doi.org/10.1016/j.vetmic.2010.09.002
  9. Fish, D. N., & Ohlinger, M. J. (2006). Antimicrobial resistance: factors and outcomes. Critical Care Clinics, 22(2), 291–311. https://doi.org/10.1016/j.ccc.2006.02.006
  10. Gati, G., Pop, C., Bruda¸ sc˘a, F., Gurzãu, A.E., & Spînu,M.(2016).The ecological risk of heavy metals in sediment from the Danube Delta. Ecotoxicology 25, 688–696.
  11. Gillings, M. R., & Stokes, H. W. (2012). Are humans increasing bacterial evolvability? Trends in ecology & evolution,27(6), 346–352. https://doi.org/10.1016/j.tree.2012.02.006
  12. Henriques, I., Tacão, M., Leite, L., Fidalgo, C., Araújo, S., Oliveira, C., & Alves, A. (2016). Co-selection of antibiotic and metal(loid) resistance in gram-negative epiphytic bacteria from contaminated salt marshes. Marine Pollution Bulletin, 109(1), 427–434. https://doi.org/10.1016/j.marpolbul.2016.05.031
  13. Ipeaiyeda A. R., & Ninawa, P. C. (2016). Sediment quality assessment and dispersion pattern of toxic metals from brewery effluent discharged into the Olosun river, Nigeria. Environ Earth Sci., 75(325), 122. https://doi.org/10.1007/s12665-015-5143-7
  14. Ju, F., Li, B., Ma, L., Wang, Y., Huang, D., & Zhang, T. (2016). Antibiotic resistance genes and human bacterial pathogens: Co-occurrence, removal, and enrichment in municipal sewage sludge digesters. Water Research, 91, 1–10. https://doi.org/10.1016/j.watres.2015.11.071
  15. Kobayashi, N., & Okamura, H. (2005). Effects of heavy metals on sea urchin embryo development. Part 2. Interactive toxic effects of heavy metals in synthetic mine effluents. Chemosphere, 61(8), 1198–1203. https://doi.org/10.1016/j.chemosphere.2005.02.071
  16. Lemire, J. A., Harrison J. J., & Turner, R. J.. (2013) Antimicrobial activity of metals: mechanisms, molecular targets and applications. Nat Rev Micro,11(6),371-84.
  17. Li, H., Luo, Y. F., Williams, B. J., Blackwell, T. S., & Xie, C. M. (2012). Structure and function of OprD protein in Pseudomonas aeruginosa: from antibiotic resistance to novel therapies. Int. J. Med. Microbiol., 302, 63–68. https://doi.org/10.1016/j.ijmm.2011.10.001
  18. Malek, M. M, Amer, F. A., Allam, A. A., Sokkary, R. H., Gheith, T. & Arafa, M. A. (2015). Occurrence of classes I and II integrons in Enterobacteriaceae collected from Zagazig University Hospitals Egypt. Frontiers in Microbiology, 6, 601.
  19. Nies, D. H. (1999). Microbial heavy-metal resistance. Appl. Microbiol. Biotechnol, 51, 730-50.
  20. Pitout J. D., Gregson, D. B., Church, D. L., Elsayed, S. & Laupland, K. B. (2005). Community-wide outbreaks of clonally related CTX-M-14 beta-lactamase-producing Escherichia coli strains in the Calgary health region. J. Clin. Microbiol, 43(6), 2844–2849.
  21. Pal, C., Bengtsson-Palme, J., Kristiansson, E., & Larsson, D. G. J. (2015). Co-occurrence of resistance genes to antibiotics, biocides and metals reveals novel insights into their co-selection potential. BMC Genomics, 16, 964.
  22. Ren, W.X., Li, P. J., Geng, Y & Li, X. J. (2009). Biological leaching of heavy metals from a contaminated soil by Aspergillus Niger. J.Hazardous Materials, 167, 1-3, 164-169.
  23. Seiler, C. &Berendonk, T.U. (2012). Heavy metal driven co-selection of antibiotic resistance in soil and water bodies impacted by agriculture and aquaculture. Frontiers in Microbiology. 3, 399. https://doi.org/10.3389/fmicb.2012.00399
  24. Sinegani A., Younessi, N. (2017). Antibiotic resistance of bacteria isolated from heavy metal-polluted soils with different land uses. Journal of global antimicrobial resistance, 10, 247–255. https://doi.org/10.1016/j.jgar.2017.05.012
  25. Su, C., Jiang, L., & Zhang, W. (2014). A review on heavy metal contamination in the soil worldwide: Situation, impact and remediation techniques. Environ Skept Crit., 3: 24–38.
  26. Tang, W., Shan, B., Zhang, H., Zhang, W., Zhao, Y. & Ding, Y. (2014). Heavy metal contamination in the surface sediments of representative limnetic ecosystems in Eastern China. Sci. Rep., 4, 7152.
  27. Varma, M. M., Thomas, W.A. & Prasad, C. (1976). Resistance to inorganic salts and antibiotics among sewage-borne Enterobacteriaceae and Achromobacteriaceae. The Journal of applied bacteriology, 41(2), 347–349.
  28. Wales, A. D., & Davies, R. H. (2015). Co-selection of resistance to antibiotics, biocides and heavy metals, and its relevance to foodborne pathogens. Antibiotics (Basel, Switzerland), 4(4), 567–604. https://doi.org/10.3390/antibiotics4040567
  29. Wei, B., & Yang, L. (2010). A review of heavy metal contaminations in urban soils, urban road dusts and agricultural soils from China. Microchemical Journal,94 (2), 99–107.
  30. Wuana, R. A., &Okieimen, F. E. (2011). Heavy metals in contaminated soils: A review of sources, chemistry, risks and best available strategies for remediation. International Scholarly Research Notices, 2011, 1–20.
  31. Yanga., Denga, W., Liub, S., Yua, X., Ghulam Raza, G., Chenb, S., Heb, L., Aob, X., Yangb, Y., Zhoub, K., Lic, B., Hand, X., Xue, X., &Zoua, L. (2020). Presence of heavy metal resistance genes in Escherichia coli and Salmonella isolates and analysis of resistance gene structure in E. coli. Journal of Global Antimicrobial Resistance, 21, 420–426.https://doi.org/10.1016/j.jgar.2020.01.009
  32. Yu, Z., Gunn, L., Wall, P. & Fanning, S. (2017). Antimicrobial resistance and its association with tolerance to heavy metals in agriculture production. Food Microbiol., 64, 23–32.
  33. Zhao, H., Xia, B., Fan, C., Zhao, P. & Shen, S. (2012). Human health risk from soil heavy metal contamination under different land uses near Dabaoshan Mine, Southern China. Sci. Total Environ., 41, 45 -54.
  34. Zhao, Y., Su, J.-Q., An, X.-L., Huang, F.-Y., Rensing, C., Brandt, K. K., & Zhu, Y.-G. (2018). Feed additives shift gut microbiota and enrich antibiotic resistance in swine gut. Sci. Total Environ, 621, 1224–1232.