Journal of the Korean institute of surface engineering (한국표면공학회지)

The Korean Institute of Surface Engineering (한국표면공학회)
권호 표지
DOI QR코드

DOI QR Code

Sterilization of Bacteria and Fungi in Cultural Heritages using Atmospheric Pressure Plasma Jet System

대기압 플라즈마 제트 시스템을 이용한 문화재 내 세균류 및 진균류의 살균

  • Jo, Sung-Il (Department of Advanced Materials Science and Engineering, Graduate School of Kangwon National University) ;
  • Park, Dong-Min (Department of Bioengineering and Technology, Graduate School of Kangwon National University) ;
  • Lee, Byeong Hoon (Kangwon Research Institute of Cultural Heritage) ;
  • So, Myoung-Gi (Department of Advanced Materials Science and Engineering, Graduate School of Kangwon National University) ;
  • Ha, Suk-Jin (Department of Bioengineering and Technology, Graduate School of Kangwon National University) ;
  • Jeong, Goo-Hwan (Department of Advanced Materials Science and Engineering, Graduate School of Kangwon National University)
  • 조성일 (강원대학교 대학원 신소재공학과) ;
  • 박동민 (강원대학교 대학원 생물공학과) ;
  • 이병훈 (강원도문화재연구소) ;
  • 소명기 (강원대학교 대학원 신소재공학과) ;
  • 하석진 (강원대학교 대학원 생물공학과) ;
  • 정구환 (강원대학교 대학원 신소재공학과)
  • Received : 2020.11.20
  • Accepted : 2020.12.11
  • Published : 2020.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Ancient cultural heritage made up of wood and organic fibers have been easily disintegrated or decomposed by various microorganisms like bacteria and fungi. Here, we demonstrate the effectiveness of an atmospheric pressure plasma jet (APPJ) system to sterilize the microorganisms in tangible cultural heritage. We collected several specimens from the surface of ancient documents and wooden artifacts. Finally, two bacteria and two fungi were prepared and sterilized using the APPJ treatment. The APPJ system is beneficial to its simple apparatus, quick operation time, and cost-effectiveness. Bacteria were almost sterilized within only 1 min treatment using 15 % O2 and applied bias voltage of 100 V. In case of the fungi, sterilization rate reached over 83 % but difficult to reach over 90 % even 10 min treatment. According to the plasma diagnostics using optical emission spectroscopy, it was found that the reactive oxygen species such as OH groups are critical for sterilization of microorganisms. Although further efforts should be performed, we believe that efficient sterilization could be realized by the simple, quick, and portable APPJ treatment system.

Keywords

References

  1. J. W. Bennett, K. G. Wunch, B. D. Faison, Use of Fungi in Biodegradation. Manual of Environmental Microbiology, ASM Press Washington (1997) 960-971.
  2. S. B. Pointing, E. B. G. Jones, A. M. Jones, Decay prevention in water logged archaeological wood using gamma irradiation, Inter. Biodeter. & Biodeg., 42 (1998) 17-24. https://doi.org/10.1016/S0964-8305(98)00041-9
  3. D. L. Kang, The Stability Appraisement on Cultural Property Material with the Replacing Fumigation Gas of Methyl Bromide, J. Conserv. Sci., 25 (2009) 283-291.
  4. N. P. McNamara, H. I. J. Black, N. A. Beresford, N. R. Parekh, Effects of acute gamma irradiation on chemical, physical and biological properties of soils. Appl. Soil Ecology, 24 (2003) 117-132. https://doi.org/10.1016/S0929-1393(03)00073-8
  5. S. Ikawa, K. Kitano, S. Hamaguchi, Effects of pH on bacterial inactivation in aqueous solutions due to low-temperature atmospheric pressure plasma application, Plasma Processes Polymer, 7 (2010) 33-42. https://doi.org/10.1002/ppap.200900090
  6. A. A. Sakr, M. A. El-Shaer, M. F. Ghaly, E. F. Abdel-Haliem, Efficacy of dielectric barrier discharge plasma in decontaminating Streptomyces colonizing specific Coptic icons, J. Cultural Heritage, 16 (2015) 848-855. https://doi.org/10.1016/j.culher.2015.02.003
  7. E. Grieten, O. Schalm, P. Tack, S. Bauters, P. Storme, N. Gauquelin, J. Caen, A. DPatelli, L. Vincze, D. Schryvers, D., 2017, Reclaiming the image of daguerreotypes: Characterization of the corroded surface before and after atmospheric plasma treatment, J. Cultural Heritage, 28 (2017) 56-64. https://doi.org/10.1016/j.culher.2017.05.008
  8. E. D. Seo, Removal of Contaminants Deposited on Surfaces of Matrices by Using Low-Temperature Argon Plasma Treatment, J. Conserv. Sci., 30 (2014) 299-306. https://doi.org/10.12654/JCS.2014.30.3.05
  9. A. fridman and L. A. Kennedy, Plasma physics and engineering, CRC Press, 2nd Ed. (2004).
  10. S. Reuter, J. Winter, A. Schmidt-Belker, H. Tresp, M. U. Hammer, K. D. Weltmann, Controlling the ambient air affected reactive species composition in the effluent of an argon plasma jet, IEEE Trans Plasma Sci., 40 (2012) 2788-2794. https://doi.org/10.1109/TPS.2012.2204280
  11. S. Wu, Y. Cao, X. Lu, The state of the art of applications of atmospheric-pressure nonequilibrium plasma jets in dentistry, IEEE Trans Plasma Sci., 44 (2016) 134-151. https://doi.org/10.1109/TPS.2015.2506658
  12. G. Pizzino, N. Irrera, M. Cucinotta, G. Pallio, F. Mannino, V. Arcoraci, F. Squadrito, D. Altavilla, A. Bitto, Oxidative Stress: Harms and Benefits for Human Health, Oxidative Medicine and Cellular Longevity (2017) 8416763.
  13. K. Katerina, C. Gonzalo, Overview of Reactive Oxygen Species, Singlet Oxygen: Applications, Biosci. and Nanosci., 1 (2016) 1-21. https://doi.org/10.1039/9781782622208-00001