ISOLATION AND CHARACTERIZATION OF TERPENTECIN, A NEW ANTITUMOR ANTIBIOTIC

TSUYOSHI TAMAMURA; TSUTOMU SAWA; KUNIO ISSHIKI; TORU MASUDA; YOSHIKO HOMMA; HIRONOBU IINUMA; HIROSHI NAGANAWA; MASA HAMADA; TOMIO TAKEUCHI; HAMAO UMEZAWA

doi:10.7164/antibiotics.38.1664

TSUYOSHI TAMAMURA, TSUTOMU SAWA, KUNIO ISSHIKI, TORU MASUDA, YOSHIKO HOMMA, HIRONOBU IINUMA, HIROSHI NAGANAWA, MASA HAMADA, TOMIO TAKEUCHI, HAMAO UMEZAWA

Author information

TSUYOSHI TAMAMURA
Institute of Microbial Chemistry
TSUTOMU SAWA
Institute of Microbial Chemistry
KUNIO ISSHIKI
Institute of Microbial Chemistry
TORU MASUDA
Institute of Microbial Chemistry
YOSHIKO HOMMA
Institute of Microbial Chemistry
HIRONOBU IINUMA
Institute of Microbial Chemistry
HIROSHI NAGANAWA
Institute of Microbial Chemistry
MASA HAMADA
Institute of Microbial Chemistry
TOMIO TAKEUCHI
Institute of Microbial Chemistry
HAMAO UMEZAWA
Institute of Microbial Chemistry

JOURNAL FREE ACCESS

1985 Volume 38 Issue 12 Pages 1664-1669

DOI https://doi.org/10.7164/antibiotics.38.1664

Details

Abstract

A new antitumor antibiotic, terpentecin was isolated from the culture broth of strain MF730-N6. Strain MF730-N6, isolated from soil, was found to belong to the genus Kitasatosporia. The antibiotic was extracted with chloroform, purified by column chromatography using silica gel and Diaion HP-20 successively, and finally purified by high performance reverse-phase thin layer chromatography. The molecular formula of terpentecin was determined to be C₂₀H₂₈O₆ (molecular weight, 364). The antibiotic inhibited the growth of Gram-positive and Gram-negative bacteria, and prolonged the survival period of mice bearing leukemia L-1210, P388 and Ehrlich ascites carcinoma.

Corresponding author

Register with J-STAGE for free!