Reduction of elevated proton leak rejuvenates mitochondria in the aged cardiomyocyte

Version of Record

Accepted for publication after peer review and revision.

Download
Cite
Share
CommentOpen annotations (there are currently 0 annotations on this page).

Version of Record published: December 15, 2020 (This version)
Accepted: December 1, 2020
Received: July 8, 2020

1. Part of Collection
Aging, Geroscience and Longevity: A Special Issue

Edited by Matt Kaeberlein et al.
Further reading

Abstract
Introduction
Results
Discussion
Materials and methods
Data availability
References
Article and author information
Metrics

Abstract

Aging-associated diseases, including cardiac dysfunction, are increasingly common in the population. However, the mechanisms of physiologic aging in general, and cardiac aging in particular, remain poorly understood. Age-related heart impairment is lacking a clinically effective treatment. Using the model of naturally aging mice and rats, we show direct evidence of increased proton leak in the aged heart mitochondria. Moreover, our data suggested ANT1 as the most likely site of mediating increased mitochondrial proton permeability in old cardiomyocytes. Most importantly, the tetra-peptide SS-31 prevents age-related excess proton entry, decreases the mitochondrial flash activity and mitochondrial permeability transition pore opening, rejuvenates mitochondrial function by direct association with ANT1 and the mitochondrial ATP synthasome, and leads to substantial reversal of diastolic dysfunction. Our results uncover the excessive proton leak as a novel mechanism of age-related cardiac dysfunction and elucidate how SS-31 can reverse this clinically important complication of cardiac aging.

Introduction

Mitochondria are both the primary source of organismal energy and the major source of cellular reactive oxygen species (ROS) and oxidative stress during aging (Dai et al., 2014). Aged cardiac mitochondria are functionally changed in redox balance and are deficient in ATP production (Lesnefsky et al., 2016). Numerous reported studies have focused on redox stress and ROS production in aging (Dai et al., 2014). However, in its simplistic form, the free radical theory of aging has become severely challenged (Pérez et al., 2009).

While more attention has been placed on mitochondrial electron leak and consequent free radical generation, proton leak is a highly significant aspect of mitochondrial energetics, as it accounts for more than 20% of oxygen consumption in the liver (Brand, 2005) and 35–50% of that in muscle in the resting state (Rolfe and Brand, 1996). There are two types of proton leak in the mitochondria: (1) constitutive, basal proton leak, and (2) inducible, regulated proton leak, including that mediated by uncoupling proteins (UCPs) (Divakaruni and Brand, 2011). In skeletal muscle, a majority of basal proton conductance has been attributed to adenine nucleotide translocase (ANT) (Brand et al., 2005). Although aging-related increased mitochondrial proton leak was detected in the mouse heart, kidneys, and liver by indirect measurement of oxygen consumption in isolated mitochondria (Harper et al., 1998; Serviddio et al., 2007), direct evidence of functional impact remains to be further investigated. Moreover, the exact site and underlying mechanisms responsible for aging-related mitochondrial proton leak are unclear.

SS-31 (elamipretide), a tetrapeptide (D-Arg-2′,6′-dimethyltyrosine-Lys-Phe-NH2), binds to cardiolipin-containing membranes (Birk et al., 2013) and improves cristae curvature (Szeto, 2014). Prevention of cytochrome c peroxidase activity and release has been proposed as its major basis of activity (Szeto, 2014; Szeto and Birk, 2014). SS-31 is highly effective in increasing resistance to a broad range of diseases, including heart ischemia reperfusion injury (Cho et al., 2007; Szeto, 2008), heart failure (Dai et al., 2013), neurodegenerative disease (Yang et al., 2009), and metabolic syndrome (Anderson et al., 2009). In aged mice, SS-31 ameliorates kidney glomerulopathy (Sweetwyne et al., 2017) and brain oxidative stress (Hao et al., 2017) and has shown beneficial effects on skeletal muscle performance (Siegel et al., 2013). We have recently shown that administration of SS-31 to 24-month-old mice for 8 weeks reverses the age-related decline in diastolic function, increasing the E/A from just above 1.0 to 1.22, restoring this parameter 35% toward that of young (5-month-old) mice (Chiao et al., 2020). However, how SS-31 benefits and protects aged cardiac cells remains unclear.

In this report, we investigated the effect and underlying mechanism of action of SS-31 on aged cardiomyocytes, especially on the mitochondrial proton leak. Using the naturally aged rodent model we provided direct evidence of increased proton leak as the primary energetic change in aged mitochondria. We further show that the inner membrane protein ANT1 mediates the augmented proton entry in the old mitochondria. Most significantly, we demonstrate that SS-31 acutely prevents the excessive mitochondrial proton entry and rejuvenates mitochondrial function through direct association with ANT1 and stabilization of the ATP synthasome.

Results

SS-31 alleviates the excessive mitochondrial proton leak in old cardiomyocytes

To examine whether SS-31 restores aging mitochondrial function, we applied the Seahorse mitochondrial stress assay to intact primary cardiomyocytes in a non-working state (not paced for contraction). The Seahorse assay revealed higher mitochondrial basal respiration in cells from old mice than that in young mouse cells (Figure 1A,C); however, the maximal respiratory rate was not significantly different (Figure 1A,D). The increased basal respiration was attributable to a higher proton leak in old cardiomyocytes (164 ± 16 in 24 month vs 82 ± 12 in young, pmol/min/800 cells, n = 7–14, p<0.01) (Figure 1A,B). Although, SS-31 has only a minor and non-significant effect on young cardiomyocytes (Figure 1—figure supplement 1), acute in vitro treatment of isolated old cardiomyocytes with SS-31 (100 nM, 1 μM, or 10 μM for 2 hr), caused reduced mitochondrial proton leak (Figure 1A,B and Figure 1—figure supplement 2), shifting their respiratory pattern closer to that of young cells. These results indicate that SS-31 directly protects aging cardiac energetics through rapid rejuvenation of mitochondrial respiration in cardiomyocytes, and in particular, by reducing proton leak.

Figure 1 with 2 supplements see all

Download asset Open asset

SS-31 alleviates the excessive mitochondrial proton leak of cardiomyocytes from 24 mo old mice.

(A) Representative Seahorse assay traces of cardiomyocytes isolated from untreated young and old mice, then exposed or not to 100 nM SS-31 for 2 hr in vitro. Aging-increased basal respiration (C), which was attributable to the augmentation of proton leak (B), but did not affect maximal respiration (D). ANT1 inhibitors Bongkrekic acid (BKA, 10 μM) and carboxyatractyloside (CAT, 20 μM) 2 hr treatment decreased the proton leak (B) and basal respiration (C) but also decreased maximal respiration (D) in old cardiomyocytes. N = 5–14 mice in each group; one-way ANOVA followed by Fisher’s LSD test. *p<0.05, **p<0.01 vs young; #p<0.05, ##p<0.01 vs old controls.

SS-31 restores the resistance to external pH gradient stress in mitochondria of old cardiomyocytes

The evaluation of mitochondrial proton leak by Seahorse assay is indirect, as it is based on the oxygen consumption rate. Thus, to directly investigate the reduction of mitochondrial proton leak in old cardiomyocytes by SS-31, we expressed the protein mt-cpYFP, a mitochondrial matrix-targeted pH indicator (Demaurex and Schwarzländer, 2016; Schwarzländer et al., 2012; Wang et al., 2016a; Wei-LaPierre et al., 2013) in the rat cardiomyocytes (Figure 2—figure supplement 1). Taking advantage of the pH sensitive character of mt-cpYFP, we developed a novel protocol to evaluate mitochondrial proton leak by exposing mitochondria to a pH gradient stress in saponin permeabilized, mt-cpYFP expressing cardiomyocytes (Figure 2A,B). The drop in mt-cpYFP 488/405 ratio is due to proton leak through the mitochondrial inner membrane into the mitochondrial matrix. To evaluate the physical properties of the mitochondrial inner membrane in the absence of mitochondrial activity, we permeabilized the cardiomyocytes in a buffer that contained no substrates, ATP, or ADP. We found that aging reduced cardiomyocyte mitochondrial resistance to a proton gradient stress (Figure 2A,B). More importantly, we found 10 μM SS-31 treatment in vitro restored cardiomyocyte mitochondrial inner membrane resistance to the pH gradient stress in the aged cardiomyocytes (Figure 2A,B). SS-31 treatment largely prevented the decline in matrix pH of old cells after the external pH was reduced to 6.9 (Figure 2—figure supplement 2) and 5.3 (Figure 2B,C) and slowed the rate of cpYFP 488/405 change after pH 6.9 (Figure 2—figure supplement 3, Figure 2D). At pH 4.5 SS-31 continued to enhance resistance to proton permeability in the treated old cells. This is unlikely to represent a biological benefit at this nonphysiologic pH, but does indicate the substantial change in physical properties of the inner membrane after interaction with SS-31 (Figure 2B). To further evaluate the kinetics of SS-31 effect on mitochondrial proton permeability, we analyzed cpYFP fluorescence ratios at various times after exposure of the saponin treated cardiomyocytes to 10 μM SS-31. SS-31 protection on the mitochondrial matrix proton entry became significant and near maximal after 7–10 min of SS-31 treatment (Figure 2E). We examined the dose effect of SS-31 on proton permeability and found near-maximal effects at 100 nM SS-31 (Figure 2F). In summary, this is the first direct evidence that aging increases mitochondrial inner membrane proton permeability in aged cardiomyocytes and that SS-31 protects mitochondria from this proton leak.

Figure 2 with 3 supplements see all

Download asset Open asset

SS-31 restores the resistance of cardiomyocytes from old rat to proton entry into the mitochondrial matrix during external pH gradient stress.

(A) Typical image of the effects of pH gradient stress on permeabilized rat cardiomyocyte mt-cpYFP fluorescence. Upper panel, young, middle panel, old, lower panel, old+SS-31 (10 μM, 3 days) visualized after exposure of the cells to pH 7.5 and, later, to pH 5.3. The excitation is 488 nm and collection is at 505–730 nm. (B) Saponin (50 μg/ml) permeabilized cardiomyocytes expressing mt-cpYFP were exposed to progressively lower external pH. Proton permeability of old mitochondria was greater than that of young mitochondria, but preincubation of old cells with 10 μM SS-31 for 3 days enhanced the mitochondrial inner membrane resistance to the pH stress. The traces were averaged from 4 to 19 experiments. The arrows indicate the changes of pH. (C) Quantitation of the SS-31 treatment effect on the mitochondrial matrix cpYFP ratio at pH 5.3. The data are from 7 to 8 min after the pH was adjusted to 5.3. The mean value of the normalized 488/405 ratio in old group is 0.59 with a SD ±0.10 and the range is from 0.46 to 0.77. N = 5–19 rats in each group, analyzed by one-way ANOVA with Dunnett’s test. (D) SS-31 decreased the rate of cpYFP 488/405 ratio drop at pH 6.9. N = 4–10 rats in each group. The rate is calculated as indicated in Figure 2—figure supplement 3. Student’s t-test was applied to determine the statistical significance. The time dependence (N = 3–4 rats in each group) (E) and dose dependence (N = 3–14 rats in each group) (F) of SS-31 protection of mitochondrial resistance to pH gradient stress are shown and analyzed by one-way ANOVA with Fisher’s LSD test. After cardiomyocyte permeabilization, 10 μM SS-31 was added for the times shown in (E) or at the doses shown in (F) for 30 min, followed by pH stress. *p<0.05, **p<0.01 vs Young; #p<0.05, ##p<0.01 vs Old.

ANT1 inhibitors restore resistance to proton leak of mitochondria in old cardiomyocytes

In search of the source of the uncoupled mitochondrial proton leak in the aged cells, we examined possible involvement of proton leakage through ATPase and mitochondrial UCPs. As expected, the ATPase inhibitor Oligomycin A failed to inhibit the proton leak in pH challenged permeabilized aged cells (Figure 3A,B). Levels of UCP2, which is the dominant isoform of UCPs in the heart, do not change with age in hearts (Figure 3—figure supplement 1). Genipin, an inhibitor of UCP2, showed no effect on the proton leak in permeabilized aged cells (Figure 3A,B). These results suggest that ATPase and UCP2 may not be the source of the excess proton leak in the aged hearts.

Figure 3 with 2 supplements see all

Download asset Open asset

ANT1 inhibitors restore resistance to proton leak of mitochondria in old rat cardiomyocytes.

ANT1 inhibitors 10 μM Bongkrekic Acid (BKA) and 20 μM carboxyatractyloside (CAT), but not 50 μM Genipin (UCP2 inhibitor) or 1 μM oligomycin A (OA, ATPase inhibitor), protected the mitochondrial matrix from decreased pH after exposure to external pH 5.3 (N = 4–19 rats in each group) (A) and reduced the rate of 488/405 decline after exposure to pH 6.9 (N = 4–10 rats in each group) (B). BKA, CAT, Genipin, or OA were added immediately after the mitochondria permeabilization. One-way ANOVA with Fisher’s LSD test was applied. *p<0.05, **p<0.01 vs Young, #p<0.05, ##p<0.01 vs Old.

Recently, the inner membrane protein ANT1 (also called AAC) was identified as the major site of proton leak in mitochondria of multiple tissues (Bertholet et al., 2019), and was shown to contribute to the majority of the proton leak in muscle cells (Brand et al., 2005). Treatment of old cardiomyocytes with either the ANT1 inhibitor bongkrekic acid (BKA) (Ruprecht et al., 2019) or carboxyatractyloside (CAT) (Pebay-Peyroula et al., 2003) completely suppressed the excess proton leak in the Seahorse assay, though unlike SS-31, they also decreased the maximal respiratory rate (Figure 1B,D), which is consistent with the effect seen in the ANT triple knockout model (Karch et al., 2019). We treated permeabilized old cardiomyocytes with the ANT1 inhibitors and examined the mt-cpYFP response to an external pH gradient using the protocol described above. BKA suppressed the proton leak in old cardiomyocytes, evidenced by the preserved 488/405 ratio at pH 5.3 and a slower 488/405 ratio decrease at pH 6.9 (Figure 3A,B). Similar inhibition was found with CAT treatment (Figure 3A,B). Taken together, these data implicate ANT1 as the major site of proton leak in aging hearts.

Considering that the inorganic phosphate carrier might be a potential source of the elevated proton entry in old mitochondria, we examined the proton permeability of old mitochondria to pH stress in a buffer that did not contain phosphate. In this buffer, the proton entry characteristics of mitochondria from old cells was indistinguishable from that of mitochondria stressed in the presence of inorganic phosphate (Figure 3—figure supplement 2).

SS-31 attenuates the excessive mitochondrial flash (mitoflash) activity of aged cardiomyocytes, while normalizing membrane potential and ROS

The mitoflash (Feng et al., 2017; Hou et al., 2014; Shen et al., 2014; Wang et al., 2008; Wang et al., 2016b; Zhang et al., 2015) is triggered by nanodomain proton influx into the mitochondrial matrix (Wang et al., 2016c). Thus, we wondered whether the increased proton leak in the old cells triggered excessive mitoflash activity. We evaluated mitoflash activity in isolated young and old rat cardiomyocytes using the indicator mt-cpYFP, as established in the previous studies noted above. The mitochondrial mitoflash activity in the cells from old (26 mo) cardiomyocytes was higher than that of young (five mo) cells (2.8 ± 0.3 in old vs 1.4 ± 0.2,/1000 μm²/100 s in young cells, n = 28–88, p<0.05). Confirming this, we detected an increase in mitoflash activity in Langendorff perfused intact aged hearts from mt-cpYFP transgenic mice (Figure 4—figure supplement 1). 1 hr treatment with SS-31 normalized the mitoflash activity in old cells to the young cell level (Figure 4D). The mitochondrial ANT1 inhibitors BKA and CAT showed super-suppression of the flash activity, reducing this frequency to half of that of young cells (Figure 4D). These data support the notion that proton leak from ANT1 triggers the mitoflash in cardiomyocytes and is responsible for the excess mitoflash activity of old cells. Moreover, the mitochondrial membrane potential, which is generally lower in old cardiomyocytes (Serviddio et al., 2007), is restored to youthful levels by SS-31 treatment (Figure 4E). Also, SS-31 reduced ROS production in the aged cardiomyocytes (Figure 4F). Thus, the reduction of mitochondrial proton leak by SS-31 is accompanied by a more youthful membrane potential and dynamic function (mitoflash), as well as less oxidative stress.

Figure 4 with 1 supplement see all

Download asset Open asset

SS-31 attenuates excessive mitoflash activity in aged rat cardiomyocytes.

(**A–C**) Mitoflash events within the regions shown in the red boxes took place at the times shown by vertical bars during the 100 s scanning time in the representative cardiomyocytes from young (A), old (B) and old+SS-31 (C) rat hearts. (D) The rate of mitoflash activity was increased in old rat cardiomyocytes compared to young, but 1 hr SS-31, Bongkrekic Acid (BKA) (10 μM) and Carboxyatractyloside (CAT) (20 μM) treatments decreased the mitoflash frequency in old cells to or below that of young cells. N = 26–87 cells from 3 to 14 rats. **p<0.05 vs young. ##p<0.05 vs old. (E) JC-1 red to green fluorescence ratio, indicative of mitochondrial membrane potential, in cells from young and old mice and old mouse cardiomyocytes treated with SS-31 (10 μM for 12 hr). N = 84–218 cells from 3 to 4 mice. p=0.06 vs young. ##p<0.01 vs old. (F) Mitochondrial ROS production in mouse cardiomyocytes measured by the fluorescence ratio of MitoSOX (5 μM, excitation 540 nm, emission >560 nm) to Mitotracker green (200 nM, excitation 488 nm, emission 505–530 nm). One-way ANOVA with Dunnett’s test was applied. N = 40–84 cells from 3 to 5 mice. **p<0.05 vs young. ##p<0.05 vs old.

SS-31 reverses increased mPTP opening in aged cardiomyocytes

Due to the close link previously established between the mitoflash and mitochondrial permeability transition pore (mPTP) opening (Hou et al., 2014), we evaluated mPTP activity by the photon-triggered mPTP opening protocol (Figure 5A; Zorov et al., 2000). Consistent with previous reports in isolated mitochondria (Hafner et al., 2010), we found that the time to mPTP opening is decreased in intact old cardiomyocytes (Figure 5B). SS-31 and the ANT1 inhibitor BKA, which stabilizes the ANT1 in the m-state open toward the mitochondrial matrix, both protect the aging-increased mitochondrial mPTP opening rate (Figure 5B), consistent with previous observations that BKA prevents the onset of the permeability transition (Halestrap et al., 1997). The ANT1 inhibitor CAT, which stabilizes ANT1 in the c-state open toward the cytosol, failed to prevent the rapid opening of the mPTP in old cells (Figure 5B), consistent with previous observations that it facilitates mPTP opening (Halestrap et al., 1997). These data indicate that SS-31 decreases mPTP opening in old cardiomyocytes.

Figure 5

Download asset Open asset

SS-31 reverses the increased speed of mitochondrial permeability transition pore (mPTP) opening in aged mouse cardiomyocytes.

(A) A typical image shows 1 Hz line-scanning photo-excitation induced mPTP opening in a cardiomyocyte loaded with the mitochondrial membrane potential (Δψ_m) dye Tetramethylrhodamine methyl ester (TMRM). The sudden decline of TMRM fluorescence with time (rightward) indicates mPTP opening and Δψ_m loss. (B) 1 hr SS-31 and Bongkrekic Acid (BKA), but not carboxyatractyloside (CAT) treatments protect the photo-excitation induced mPTP opening. Quantification of time to mPTP opening from 418 to 658 mitochondria from 19 to 32 cells isolated from 3 to 4 mice in each group. One-way ANOVA with Dunnett’s test was applied. **p<0.01 vs young, ##p<0.01 vs old.

SS-31 associates directly with ANT1 and the ATP synthasome

To further investigate the mechanism of SS-31 protection on the proton leak, we used biotinylated SS-31 to evaluate whether SS-31 directly interacts with the ANT1 protein. Hearts were disrupted by douncing, after a low speed spin to remove fragments, mitochondria were collected by high speed spin and disrupted in digitonin, to create lipid rafts containing their associated proteins, a protocol commonly used to prepare mitochondrial supercomplexes (Johnson et al., 2013). This preparation was incubated with SS-31-biotin or biotin only, followed by incubation with streptavidin beads. After washing, the bead-bound fraction was eluted with excess SS-31 and analyzed by Western blotting. Biotin-SS-31 pulled down ANT1, and free SS-31 competed with the biotin-SS-31 binding to ANT1 (Figure 6A,B, Figure 6—figure supplement 1). Most notably, both BKA and CAT inhibited binding of biotin-SS-31 to ANT1 (Figure 6A,B). This competition was observed even at BKA and CAT concentrations in the tens of nanomolar range (data not shown), which is consistent with their reported Kd of binding to ANT1 (Vignais et al., 1976). Biotin-SS-31 pulldown of ANT1 was not inhibited by Genipin or Oligomycin A (Figure 6A,B). These data indicate that SS-31 associates closely with the ANT1 protein. Moreover, native gel and ATPase blot analysis showed that SS-31 stabilized the ATP synthasome, of which ANT1 and ATPase are critical members (Ko et al., 2003; Figure 6D,E). However, SS-31 treatment did not produce a detectable increase in mitochondrial complex proteins by Coomassie blue staining (Figure 6C). Taken together, these data suggest that SS-31 interacts directly with ANT1 and stabilizes the ATP synthasome in old cardiomyocytes.

Figure 6 with 1 supplement see all

Download asset Open asset

SS-31 interacts with ANT1 and stabilizes the ATP synthasome in the old mouse heart mitochondria.

(**A, B**) Biotin-SS-31 pulldown shows the association of biotin-SS-31 to ANT1. Free SS-31 competes with this interaction, while Bongkrekic Acid (BKA) and carboxyatractyloside (CAT) inhibit the interaction of biotin-SS-31 with ANT1. Panel A shows a representative Western blot. N = 6 mice in each group. One-way ANOVA with Fisher’s LSD test was applied. **p<0.01 vs Biotin control, #p<0.05, ##p<0.01 vs Biotin-SS31 pulldown. (C) Coomassie blue staining of isolated mitochondria in a native gel. (D) Left panel is the total protein loading control for the Native gel blot. (**D, E**) Native gel blotting shows that 10 μM SS-31 stabilizes the mitochondrial synthasome (Syn) in isolated mitochondria. The Syn is highlighted in the red box. The Syn and ATPase Dimer (D) and Monomer (M) were labeled using anti-ATP5A. N = 8 mice in each group. One-way ANOVA with Fisher’s LSD test was applied. *p<0.05 vs young, #p<0.05 vs old.

Discussion

In this report, we have shown direct evidence of increased proton leak in the aged mitochondria as a primary energetic disturbance and that the increased proton entry in old cardiomyocytes likely takes place through ANT1. Moreover, we demonstrated that SS-31 prevents the proton entry to the mitochondrial matrix and rejuvenates mitochondrial function through direct interaction with ANT1 and stabilization of the ATP synthasome. During aging, the pathological augmented and sustained basal proton leak burdens the mitochondrial work load, resulting in a decline in respiratory efficiency. Blocking this pathological proton leak induced by aging benefits the mitochondria and the heart (Figure 7). We suggest that the restoration of aged mitochondrial function that is conferred by SS-31 is directly attributable to this effect. However, the resulting enhancement in diastolic function is likely to require downstream changes, as the functional benefit took up to 8 weeks to reach full effect, and required post-translational modifications of contractile protein elements (Chiao et al., 2020). It is increasingly recognized that mitochondrial function, including redox status and energetics, has far-reaching effects, including epigenetic alterations and post-translational modifications (Olgar et al., 2019).

Figure 7

Download asset Open asset

Schematic of the mechanism of SS-31 protection of proton leak and rejuvenation of mitochondrial function.

Due to increased mitochondrial proton leak, the mitochondria work harder to maintain ATP production, and thus the work load is increased in the aged heart.

ANT1 appears to mediate the pathological mitochondrial proton leak in the aged mouse heart. Although an increased mitochondrial proton leak in the aged heart was previously suggested by indirect oxygen consumption measurement (Serviddio et al., 2007), the site of this augmented proton leak in aging mitochondria has remained a puzzle. We directly evaluated the proton leak using the mitochondrial matrix-targeted pH indicator (mt-cpYFP) and provide evidence that implicates ANT1, the ATP/ADP translocator, as responsible for the pathologically increased proton leak in aged cardiomyocytes. This does not necessarily implicate the ADP/ATP translocase mechanism itself in the proton leak, as in the unenergetic state in which we examined the mitochondrial pH resistance, there would be no ADP/ATP transport activity. Our result is, however, supported by a recent report that proton transport is an integral function of ANT1 (Bertholet et al., 2019). Because the ANT1 protein level is not increased in the aged heart (it is, in fact, mildly but significantly decreased, Figure 3—figure supplement 1), the aging-augmented proton leak through ANT1 must be through altered transport activity or conformational change. Both the inhibitors BKA (locking ANT1 in the matrix face state [Ruprecht et al., 2019]) and CAT (locking ANT1 in the cytosol face state [Pebay-Peyroula et al., 2003]) suppressed the proton leak in the aged cardiomyocytes, suggesting that constraining the conformational state in either position, or otherwise blocking the proton pore reduces ANT1 proton translocation.

Most interestingly, for the first time, we showed that a novel drug, SS-31 (elamipretide), now in clinical trials, prevents the augmented mitochondrial proton leak, rejuvenates mitochondrial function, and reverses aging-related cardiac dysfunction. Mechanistically, we found that SS-31 directly interacts with ANT1 and stabilizes formation of the ATP synthasome. This would seem surprising, given the prior belief that SS-31 affects mitochondria via binding to cardiolipin. However, the notion that SS-31 prevents the proton leak by direct interaction at the pore ‘pocket’ of ANT1 is supported by recent observations based on cross-linking ‘interactome’ mass-spectroscopy that showed that SS-31 is in intimate proximity to two lysine amino acid residues in the water filled cavity of the ANT1 protein (Chavez et al., 2020). Moreover, the cross-linking data suggested that this interaction could have structural consequences and may stabilize the m-state of ANT1 (Chavez et al., 2020). Our observation that both BKA and CAT blocked the SS-31 interaction with ANT1 suggests that SS-31 interacts with ANT1 independent of the ANT1 face to m-state (matrix facing) or c-state (cytoplasmic-facing). These results, and prior evidence of the critical role of ANT1 in mitochondrial health and function (Liu and Chen, 2013), warrant further high resolution structural study of the ANT transporter.

It has recently been shown that SS-31 alters surface electrostatic properties of the mitochondrial inner membrane (Mitchell et al., 2019). The consequences of this effect could include alteration of the channel ion gating properties of the ANT, including conformational changes secondary to enhanced supercomplex and ATP synthasome complex stability. SS-31 effects on stabilization of mitochondrial synthasome (Figure 6D,E) could directly contribute to the enhanced efficiency of mitochondrial respiration that is seen in muscle of SS-31 treated old animals (Siegel et al., 2013) and the improvement in performance of humans with primary mitochondrial myopathy (Karaa et al., 2018). As considerable prior literature indicates that SS-31 interacts with cardiolipin, this interaction would also affect the ATP synthasome, as all the components of the ATP synthasome are ‘floating’ in the inner membrane lipid membrane (of which about 20% is cardiolipin). It is possible that this directly affects stability of the synthasome components. Alternatively, the effect could be due to the change in inner membrane electrostatic properties that has recently been shown to result from incorporation of SS-31 into the inner membrane (Mitchell et al., 2019), as this could indirectly stabilize the ATP synthasome.

The restoration of membrane potential by SS-31 in the old mitochondria (Figure 4E) can be attributed to the suppression of proton leak. However, SS-31 also decreased ROS production (Figure 4F) in the aged cardiomyocytes. It is unclear if the reduced ROS production is associated with modification of the ANT1 shown here or through a parallel mechanism. Blocking this pathological proton leak induced by aging will benefit the mitochondria and the heart. This is not in conflict with the ‘uncoupling to survive hypothesis’, which arises from the positive correlations between increase proton leak, reduced ROS, and increased lifespan (Brand, 2000). This reduced ROS production is interpreted as resulting from decreased electromotive force and consequent reduced electron leak during transport through the respiratory chain. However, SS-31 through its interaction with cardiolipin, abundant in the inner membrane, can improve the efficiency of electron transfer, especially by its known interaction with the heme group of cytochrome c (Szeto, 2014; Szeto and Birk, 2014), thereby reducing ROS production, even as the aged mitochondrial membrane potential is increased. Thus, our data support the conclusion that SS-31 interaction with multiple inner membrane proteins enhances the performance of multiple facets of respiratory mechanics.

In summary, our study reveals that the adenine nucleotide transporter is the most likely candidate responsible for the elevated proton leak in old cardiomyocytes and that SS-31 acutely and directly interacts with the transporter, preventing the proton leak and rejuvenating mitochondrial function in the aged cardiomyocytes. The improved mitochondrial function leads to complex secondary changes to effect enhanced diastolic function in the aged heart. These findings provide a novel insight for better understanding of the mechanisms of cardiac aging and establish the novel concept that decreasing the pathological proton leak in the aging heart restores mitochondrial function, ultimately reversing cardiac dysfunction in aging.

Materials and methods

Key resources table

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Strain, strain background (M. musculus; male and female)	C57BL/6J	National Institute of Aging Charles River colony	RRID:IMSR_JAX:000664	See Materials and method
Strain, strain background (Rattus norvegicus)	F344	National Institute of Aging F344 rats		See Materials and method
Genetic reagent (M. musculus)	mt-cpYFP	Wang lab, PMID:25252178		See Materials and method
Transfected construct (Adenovirus)	mt-cpYFP	Wang lab, PMID:25252178		Adenovirus to transfect and express mt-cpYFP
Antibody	anti- ANT1	Abcam	Cat# ab102032	1:3000
Antibody	anti-UCP2	Cell signaling technology	Cat# 89326S	1:2000
Antibody	anti-ATP5a	Abcam	Cat# ab14748	1:3000
Peptide, recombinant protein	SS-31 peptide (Elamipretide)	Stealth BioTherapeutics
Commercial assay or kit	Seahorse XF Cell Mito Stress Test Kit	Aligent/Seahorse Bioscience	103015–100
Commercial assay or kit	MitoSOX Red	ThermoFisher	M36008
Chemical compound, drug	Bongkrekic acid	Cayman Chemical	19079
Chemical compound, drug	Carboxyatractyloside	Cayman Chemical	21120
Chemical compound, drug	Genipin	Sigma	G4796
Chemical compound, drug	Oligomycin A	Sigma	75351
Chemical compound, drug	carbonyl cyanide-p-trifluoromethoxyphenylhydrazone	Sigma	C2920
Chemical compound, drug	Antimycin A	Sigma	A8674
Chemical compound, drug	Rotenone	Sigma	R8875
Commercial assay or kit	MitoTracker Green	ThermoFisher	M7514
Commercial assay or kit	JC-1 Dye	ThermoFisher	T3168
Commercial assay or kit	Biotin	ThermoFisher	B20656
Commercial assay or kit	Streptavidin Agarose beads	ThermoFisher	20349
Commercial assay or kit	Pierce Reversible Protein Stain Kit for PVDF Membranes	Thermo Scientific	24585
Commercial assay or kit	BCA protein assay	Thermo Scientific	23225
Commercial assay or kit	SuperSignal West Pico PLUS Chemiluminescent Substrate	Thermo Scientific	34580
Software, algorithm	Graphpad Prism	Graphpad	RRID:SCR_002798
Software, algorithm	AlphaView Software	ProteinSimple

Animals

All the animal procedures were approved by the Institutional Animal Care and Use Committee at the University of Washington and conform to the NIH guidelines (Guide for the care and use of laboratory animals). The use of mice and rats have been approved by IACUC under animal protocols 2174–23 and 2654–03. Young (4–6-month-old) and aged (24–26-month-old) C57BL/6 mice (Charles River colony) and F344 rats (young, 5-7-month old; aged, 25–30-month-old) were obtained from the National Institute of Aging Rodent Resource. The mt-cpYFP transgenic C57BL/6 mice were housed until reaching the age described.

Isolation of adult mouse and rat cardiomyocytes

View detailed protocol

Single ventricular myocytes were enzymatically isolated from mouse and rat hearts as described previously (Zhang et al., 2013; Zhang et al., 2017). The rod-shaped cardiomyocytes were collected by allowing cells to settle down and adhere to laminin coated-24 well Seahorse plates for intact cell oxygen consumption test, or to glass coverslips for confocal imaging.

Seahorse assay

Request a detailed protocol

The XF24e Extracellular Flux Analyzer (Seahorse Bioscience) was used for measuring oxygen consumption in intact resting cardiomyocyte, with XF assay medium containing 5 mM glucose and 1 mM pyruvate. Oligomycin A (OA, 2.5 μM), carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP, 1 μM), and antimycin A (AA, 2.5 μM) plus 1 μM rotenone (Rot) were added in three sequential injections, as reported (Zhang et al., 2017).

Confocal imaging

Request a detailed protocol

We used a Zeiss 510 (Zeiss, Germany) or Leica SP8 (Leica, Germany) for confocal imaging at room temperature. The cells were placed in modified Tyrode’s solution (in mM: 138 NaCl, 0.5 KCl, 20 HEPES, 1.2 MgSO₄, 1.2 KH₂PO₄, 5 Glucose, 1 CaCl₂, pH 7.4). For mitochondrial flashes, mt-cpYFP expressing cells were exposed to alternating excitation at 405 and 488 nm and emission collected at >505 nm. Time-lapse 2D images were collected at a rate of 1 second per frame. For mitochondrial superoxide quantitation, we used the ratio of MitoSOX Red (5 μM, excited at 540 nm with emission collected at >560 nm) to mitoTracker Green (200 nM, excited at 488 nm and emission collected at 505–530 nm). For mitochondrial membrane potential measurement, JC-1 was excited at 488 nm and emission collected at 510–545 nm and 570–650 nm. For photon triggered mPTP opening, the cells were loaded with 120 nM Tetramethylrhodamine methyl ester (TMRM) and line scanned at 1 Hz as described previously (Zorov et al., 2000).

Cell permeabilization and pH stress

Request a detailed protocol

Rat cardiomyocytes were cultured with mt-cpYFP adenovirus (Wang et al., 2016b) for 3 days in M199 medium. After incubation in Ca²⁺-free Tyrode’s solution for 30 min, the medium was changed to a solution of 100 mM potassium aspartate, 20 mM KCl, 10 mM glutathione, 10 mM KH₂PO₄, 0.1 mM EGTA, 8% dextran 40,000, pH 7.5, with 50 μg/ml saponin for 30 s and then maintained in saponin-free internal solution (Lukyanenko and Gyorke, 1999). The pH of the solution containing the permeabilized cells was then progressively lowered by addition of HCl in quantities previously titrated to result in pH 7.3, 6.9, 5.3, and 4.5, with 8 min between each step. The permeabilized cells were excited using the same settings as for mt-cpYFP above, but using a time-lapse of 6 seconds per frame. The ratio of emission fluorescence at 488 nm from 405 nm excitation indicated the mitochondrial pH change (Wei-LaPierre et al., 2013) and was normalized to a starting (pH 7.5) arbitrary value of 1.0, so as to normalize differences due to variability of the intensity of laser excitation and emission collection between different experiments. Bongkrekic acid (BKA) 10 μM (Adams et al., 2000), CAT 20 μM (Winter et al., 2016), Genipin 50 μM (Wang et al., 2017), OA were added after cell permeabilization.

Western blots

Request a detailed protocol

Heart tissue was lysed with RIPA buffer containing a protease inhibitor cocktail (Chiao et al., 2016). Protein samples were denatured and separated via NuPAGE Bis-Tris gel, and transferred to PVDF membranes. The blots were probed with primary antibodies: ANT1 (Abcam, ab102032, 1:3000), UCP2 (Cell Signaling Technology, 89326S, 1:2000) followed by appropriate secondary antibodies.

Biotin-SS-31 pulldown and blot analysis

Request a detailed protocol

Hearts were chunked and dounce homogenized in mitochondrial isolation buffer (MIB, in mM: 300 sucrose, 10 Na-HEPES, 0.200 EDTA, pH 7.4) and centrifuged at 800 g for 10 min. The supernatants were centrifuged at 8000 g for 15 min to purify mitochondria. Digitonin was added to the mitochondria at a ratio of Digitonin: protein = 6:1 to break down the membrane system. Treatment drugs were added 30 min before addition of 10 μM biotin-SS-31 (Biotin-D-Arg-dimethyl Tyr-Lys-Phe-NH2) or biotin control (Thermo, B20656). Streptavidin Agarose beads (Thermo, 20349) were added and incubated for 2 hrs at room temperature. The beads were washed with MIB three times and then eluted by 50 μM SS-31. The eluates were boiled with LDS protein loading buffer (Thermo, NP0008) and loaded on NuPAGE for gel electrophoresis and Western blotting with antibody for ANT1 (Abcam, ab102032, 1:3000). In some experiments, after electrophoresis, gels were silver stained using a Pierce Silver Stain Kit (Thermo, #24612).

Native coomassie blue staining and blotting

Request a detailed protocol

Mitochondria from mouse hearts were isolated as described previously (Marcu et al., 2012). Mitochondria (100 µg) were solubilized in 4x NativePAGE Sample Buffer containing 5% digitonin and 5% coomassie blue G-250. The samples were loaded on NativePAGE Novex 3–12% Gel and run at 100 V for 1 hr, then at 300 V for 2 hr. For coomassie blue staining, gels were stained with 0.1% Coomassie Brilliant Blue overnight and destained with destaining solution (H₂O: Methanol: Acetic Acid = 5:4:1) 5 times at 20 min intervals. For native blotting, gels were transferred to PVDF membranes at 25 V in 4°C overnight and incubated with ATP5a antibody (Abcam, ab14748, 1:3000), followed by anti-mouse secondary antibody.

Perfused mouse heart confocal imaging

Request a detailed protocol

mt-cpYFP transgenic mice were anesthetized with pentobarbital (150 mg/kg). The heart was removed, cannulated via the ascending aorta, and put on a modified perfusion system and in a custom made chamber on the confocal stage as previously reported (Zhang et al., 2018; Zhang et al., 2017). The perfusion was maintained under a constant flow (~2 ml/min) with O₂/CO₂-bubbled KHB solution (in mM: 118 NaCl, 0.5 EDTA, 10 D-glucose, 5.3 KCl, 1.2 MgCl₂, 25 NaHCO₃, 0.5 pyruvate, and 2 CaCl₂, pH 7.4) at 37°C. To minimize motion artifact during imaging, 10 µM (-)-Blebbistatin (Toronto Research Chemicals) was included. During imaging, the left ventricle was gently pressed to further suppress motion artifact. Mitoflashes were imaged using the procedure described above.

Data statistics

Request a detailed protocol

Data are shown as mean ± SEM. For the multiple groups comparisons one-way ANOVA was applied followed with appropriate test. Student’s t-test was used to determine the statistical significance between two group comparisons. p<0.05 was considered statistically significant.

Data availability

Data available in Dryad: https://doi.org/10.5061/dryad.fqz612jqs.

The following data sets were generated

1. Zhang H
2. Alder NN
3. Wang W
4. Hazel SH
5. Marcinek DJ
6. Rabinovitch PS
(2020) Dryad Digital Repository
Reduction of Elevated Proton Leak Rejuvenates Mitochondria in the Aged Cardiomyocyte.
https://doi.org/10.5061/dryad.fqz612jqs

References

1. Adams JW
2. Pagel AL
3. Means CK
4. Oksenberg D
5. Armstrong RC
6. Brown JH
(2000) Cardiomyocyte apoptosis induced by Galphaq signaling is mediated by permeability transition pore formation and activation of the mitochondrial death pathway
Circulation Research 87:1180–1187.
https://doi.org/10.1161/01.RES.87.12.1180
- PubMed
- Google Scholar
1. Anderson EJ
2. Lustig ME
3. Boyle KE
4. Woodlief TL
5. Kane DA
6. Lin CT
7. Price JW
8. Kang L
9. Rabinovitch PS
10. Szeto HH
11. Houmard JA
12. Cortright RN
13. Wasserman DH
14. Neufer PD
(2009) Mitochondrial H2O2 emission and cellular redox state link excess fat intake to insulin resistance in both rodents and humans
Journal of Clinical Investigation 119:573–581.
https://doi.org/10.1172/JCI37048
- PubMed
- Google Scholar
1. Bertholet AM
2. Chouchani ET
3. Kazak L
4. Angelin A
5. Fedorenko A
6. Long JZ
7. Vidoni S
8. Garrity R
9. Cho J
10. Terada N
11. Wallace DC
12. Spiegelman BM
13. Kirichok Y
(2019) H⁺ transport is an integral function of the mitochondrial ADP/ATP carrier
Nature 571:515–520.
https://doi.org/10.1038/s41586-019-1400-3
- PubMed
- Google Scholar
1. Birk AV
2. Liu S
3. Soong Y
4. Mills W
5. Singh P
6. Warren JD
7. Seshan SV
8. Pardee JD
9. Szeto HH
(2013) The mitochondrial-targeted compound SS-31 re-energizes ischemic mitochondria by interacting with cardiolipin
Journal of the American Society of Nephrology 24:1250–1261.
https://doi.org/10.1681/ASN.2012121216
- PubMed
- Google Scholar
1. Brand MD
(2000) Uncoupling to survive? the role of mitochondrial inefficiency in ageing
Experimental Gerontology 35:811–820.
https://doi.org/10.1016/S0531-5565(00)00135-2
- PubMed
- Google Scholar
1. Brand MD
(2005) The efficiency and plasticity of mitochondrial energy transduction
Biochemical Society Transactions 33:897–904.
https://doi.org/10.1042/BST0330897
- PubMed
- Google Scholar
1. Brand MD
2. Pakay JL
3. Ocloo A
4. Kokoszka J
5. Wallace DC
6. Brookes PS
7. Cornwall EJ
(2005) The basal proton conductance of mitochondria depends on Adenine nucleotide translocase content
Biochemical Journal 392:353–362.
https://doi.org/10.1042/BJ20050890
- PubMed
- Google Scholar
1. Chavez JD
2. Tang X
3. Campbell MD
4. Reyes G
5. Kramer PA
6. Stuppard R
7. Keller A
8. Zhang H
9. Rabinovitch PS
10. Marcinek DJ
(2020) Mitochondrial protein interaction landscape of SS-31
PNAS 31:739128.
https://doi.org/10.1101/739128
- Google Scholar
1. Chiao YA
2. Kolwicz SC
3. Basisty N
4. Gagnidze A
5. Zhang J
6. Gu H
7. Djukovic D
8. Beyer RP
9. Raftery D
10. MacCoss M
11. Tian R
12. Rabinovitch PS
(2016) Rapamycin transiently induces mitochondrial remodeling to reprogram energy metabolism in old hearts
Aging 8:314–327.
https://doi.org/10.18632/aging.100881
- PubMed
- Google Scholar
Preprint
1. Chiao YA
2. Zhang H
3. Sweetwyne M
4. Whitson J
5. Ting YS
6. Basisty N
7. Pino L
8. Quarles E
9. Nguyen NH
10. Campbell M
(2020) Late-life restoration of mitochondrial function reverses cardiac dysfunction in old mice
bioRxiv.
https://doi.org/10.1101/2020.01.02.893008
- Google Scholar
1. Cho J
2. Won K
3. Wu D
4. Soong Y
5. Liu S
6. Szeto HH
7. Hong MK
(2007) Potent mitochondria-targeted peptides reduce myocardial infarction in rats
Coronary Artery Disease 18:215–220.
https://doi.org/10.1097/01.mca.0000236285.71683.b6
- PubMed
- Google Scholar
1. Dai DF
2. Hsieh EJ
3. Chen T
4. Menendez LG
5. Basisty NB
6. Tsai L
7. Beyer RP
8. Crispin DA
9. Shulman NJ
10. Szeto HH
11. Tian R
12. MacCoss MJ
13. Rabinovitch PS
(2013) Global proteomics and pathway analysis of pressure-overload-induced heart failure and its attenuation by mitochondrial-targeted peptides
Circulation: Heart Failure 6:1067–1076.
https://doi.org/10.1161/CIRCHEARTFAILURE.113.000406
- PubMed
- Google Scholar
(2014) Mitochondrial oxidative stress in aging and healthspan
Longevity & Healthspan 3:6.
https://doi.org/10.1186/2046-2395-3-6
- Google Scholar
1. Demaurex N
2. Schwarzländer M
(2016) Mitochondrial flashes: dump superoxide and dance with protons now
Antioxidants & Redox Signaling 25:550–551.
https://doi.org/10.1089/ars.2016.6819
- PubMed
- Google Scholar
1. Divakaruni AS
2. Brand MD
(2011) The regulation and physiology of mitochondrial proton leak
Physiology 26:192–205.
https://doi.org/10.1152/physiol.00046.2010
- PubMed
- Google Scholar
Book
1. Feng G
2. Liu B
3. Hou T
4. Wang X
5. Cheng H
(2017) Mitochondrial flashes: elemental signaling events in eukaryotic cells
In: Singh H, Sheu S. S, editors. Handbook of Experimental Pharmacology. Springer. pp. 403–422.
https://doi.org/10.1007/164_2016_129
- Google Scholar
1. Hafner AV
2. Dai J
3. Gomes AP
4. Xiao C-Y
5. Palmeira CM
6. Rosenzweig A
7. Sinclair DA
(2010) Regulation of the mPTP by SIRT3-mediated deacetylation of CypD at lysine 166 suppresses age-related cardiac hypertrophy
Aging 2:914–923.
https://doi.org/10.18632/aging.100252
- Google Scholar
(1997) Oxidative stress, thiol reagents, and membrane potential modulate the mitochondrial permeability transition by affecting nucleotide binding to the Adenine nucleotide translocase
Journal of Biological Chemistry 272:3346–3354.
https://doi.org/10.1074/jbc.272.6.3346
- Google Scholar
1. Hao ZH
2. Huang Y
3. Wang MR
4. Huo TT
5. Jia Q
6. Feng RF
7. Fan P
8. Wang JH
(2017) SS31 ameliorates age-related activation of NF-κB signaling in senile mice model, SAMP8
Oncotarget 8:1983–1992.
https://doi.org/10.18632/oncotarget.14077
- PubMed
- Google Scholar
(1998) Age-related increase in mitochondrial proton leak and decrease in ATP turnover reactions in mouse hepatocytes
American Journal of Physiology-Endocrinology and Metabolism 275:E197–E206.
https://doi.org/10.1152/ajpendo.1998.275.2.E197
- Google Scholar
1. Hou T
2. Wang X
3. Ma Q
4. Cheng H
(2014) Mitochondrial flashes: new insights into mitochondrial ROS signalling and beyond
The Journal of Physiology 592:3703–3713.
https://doi.org/10.1113/jphysiol.2014.275735
- Google Scholar
1. Johnson SC
2. Yanos ME
3. Kayser EB
4. Quintana A
5. Sangesland M
6. Castanza A
7. Uhde L
8. Hui J
9. Wall VZ
10. Gagnidze A
11. Oh K
12. Wasko BM
13. Ramos FJ
14. Palmiter RD
15. Rabinovitch PS
16. Morgan PG
17. Sedensky MM
18. Kaeberlein M
(2013) mTOR inhibition alleviates mitochondrial disease in a mouse model of leigh syndrome
Science 342:1524–1528.
https://doi.org/10.1126/science.1244360
- PubMed
- Google Scholar
1. Karaa A
2. Haas R
3. Goldstein A
4. Vockley J
5. Weaver WD
6. Cohen BH
(2018) Randomized dose-escalation trial of elamipretide in adults with primary mitochondrial myopathy
Neurology 90:e1212–e1221.
https://doi.org/10.1212/WNL.0000000000005255
- PubMed
- Google Scholar
1. Karch J
2. Bround MJ
3. Khalil H
4. Sargent MA
5. Latchman N
6. Terada N
7. Peixoto PM
8. Molkentin JD
(2019) Inhibition of mitochondrial permeability transition by deletion of the ANT family and CypD
Science Advances 5:eaaw4597.
https://doi.org/10.1126/sciadv.aaw4597
- PubMed
- Google Scholar
1. Ko YH
2. Delannoy M
3. Hullihen J
4. Chiu W
5. Pedersen PL
(2003) Mitochondrial ATP synthasome. Cristae-enriched membranes and a multiwell detergent screening assay yield dispersed single complexes containing the ATP synthase and carriers for pi and ADP/ATP
The Journal of Biological Chemistry 278:12305–12309.
https://doi.org/10.1074/jbc.C200703200
- PubMed
- Google Scholar
(2016) Mitochondrial metabolism in aging heart
Circulation Research 118:1593–1611.
https://doi.org/10.1161/CIRCRESAHA.116.307505
- Google Scholar
1. Liu Y
2. Chen XJ
(2013) Adenine nucleotide translocase, mitochondrial stress, and degenerative cell death
Oxidative Medicine and Cellular Longevity 2013:1–10.
https://doi.org/10.1155/2013/146860
- Google Scholar
1. Lukyanenko V
2. Gyorke S
(1999) ^Ca2+ sparks and ^Ca2+ waves in saponin-permeabilized rat ventricular myocytes
The Journal of Physiology 521 Pt 3:575–585.
https://doi.org/10.1111/j.1469-7793.1999.00575.x
- PubMed
- Google Scholar
(2012) Multi-parameter measurement of the permeability transition pore opening in isolated mouse heart mitochondria
Journal of Visualized Experiments 1:4131.
https://doi.org/10.3791/4131
- Google Scholar
Preprint
1. Mitchell W
2. Ng EA
3. Tamucci JD
4. Boyd K
5. Sathappa M
6. Coscia A
7. Pan M
8. Han X
9. Eddy NA
10. May ER
(2019) Molecular mechanism of action of mitochondrial therapeutic SS-31 (Elamipretide): Membrane interactions and effects on surface electrostatics
bioRxiv.
https://doi.org/10.1101/735001
- Google Scholar
1. Olgar Y
2. Tuncay E
3. Turan B
(2019) Mitochondria-Targeting antioxidant provides cardioprotection through regulation of cytosolic and mitochondrial ^Zn2+levels with Re-Distribution of ^Zn2+-Transporters in aged rat cardiomyocytes
International Journal of Molecular Sciences 20:3783.
https://doi.org/10.3390/ijms20153783
- Google Scholar
(2003) Structure of mitochondrial ADP/ATP carrier in complex with carboxyatractyloside
Nature 426:39–44.
https://doi.org/10.1038/nature02056
- PubMed
- Google Scholar
1. Pérez VI
2. Bokov A
3. Remmen HV
4. Mele J
5. Ran Q
6. Ikeno Y
7. Richardson A
(2009) Is the oxidative stress theory of aging dead?
Biochimica et Biophysica Acta (BBA) - General Subjects 1790:1005–1014.
https://doi.org/10.1016/j.bbagen.2009.06.003
- Google Scholar
1. Rolfe DF
2. Brand MD
(1996) Contribution of mitochondrial proton leak to skeletal muscle respiration and to standard metabolic rate
American Journal of Physiology-Cell Physiology 271:C1380–C1389.
https://doi.org/10.1152/ajpcell.1996.271.4.C1380
- Google Scholar
1. Ruprecht JJ
2. King MS
3. Zögg T
4. Aleksandrova AA
5. Pardon E
6. Crichton PG
7. Steyaert J
8. Kunji ERS
(2019) The molecular mechanism of transport by the mitochondrial ADP/ATP carrier
Cell 176:435–447.
https://doi.org/10.1016/j.cell.2018.11.025
- Google Scholar
1. Schwarzländer M
2. Murphy MP
3. Duchen MR
4. Logan DC
5. Fricker MD
6. Halestrap AP
7. Müller FL
8. Rizzuto R
9. Dick TP
10. Meyer AJ
11. Sweetlove LJ
(2012) Mitochondrial ‘flashes’: a radical concept repHined
Trends in Cell Biology 22:503–508.
https://doi.org/10.1016/j.tcb.2012.07.007
- Google Scholar
(2007) Bioenergetics in aging: mitochondrial proton leak in aging rat liver, kidney and heart
Redox Report 12:91–95.
https://doi.org/10.1179/135100007X162112
- Google Scholar
1. Shen EZ
2. Song CQ
3. Lin Y
4. Zhang WH
5. Su PF
6. Liu WY
7. Zhang P
8. Xu J
9. Lin N
10. Zhan C
11. Wang X
12. Shyr Y
13. Cheng H
14. Dong MQ
(2014) Mitoflash frequency in early adulthood predicts lifespan in Caenorhabditis elegans
Nature 508:128–132.
https://doi.org/10.1038/nature13012
- PubMed
- Google Scholar
1. Siegel MP
2. Kruse SE
3. Percival JM
4. Goh J
5. White CC
6. Hopkins HC
7. Kavanagh TJ
8. Szeto HH
9. Rabinovitch PS
10. Marcinek DJ
(2013) Mitochondrial-targeted peptide rapidly improves mitochondrial energetics and skeletal muscle performance in aged mice
Aging Cell 12:763–771.
https://doi.org/10.1111/acel.12102
- Google Scholar
1. Sweetwyne MT
2. Pippin JW
3. Eng DG
4. Hudkins KL
5. Chiao YA
6. Campbell MD
7. Marcinek DJ
8. Alpers CE
9. Szeto HH
10. Rabinovitch PS
11. Shankland SJ
(2017) The mitochondrial-targeted peptide, SS-31, improves glomerular architecture in mice of advanced age
Kidney International 91:1126–1145.
https://doi.org/10.1016/j.kint.2016.10.036
- Google Scholar
1. Szeto HH
(2008) Mitochondria-Targeted Cytoprotective Peptides for Ischemia–Reperfusion Injury
Antioxidants & Redox Signaling 10:601–620.
https://doi.org/10.1089/ars.2007.1892
- Google Scholar
1. Szeto HH
(2014) First-in-class cardiolipin-protective compound as a therapeutic agent to restore mitochondrial bioenergetics
British Journal of Pharmacology 171:2029–2050.
https://doi.org/10.1111/bph.12461
- Google Scholar
1. Szeto HH
2. Birk AV
(2014) Serendipity and the Discovery of Novel Compounds That Restore Mitochondrial Plasticity
Clinical Pharmacology & Therapeutics 96:672–683.
https://doi.org/10.1038/clpt.2014.174
- Google Scholar
(1976) Binding of radioactively labeled carboxyatractyloside, atractyloside and bongkrekic acid to the ADP translocator of potato mitochondria
Biochimica et Biophysica Acta (BBA) - Bioenergetics 440:688–696.
https://doi.org/10.1016/0005-2728(76)90051-7
- Google Scholar
1. Wang W
2. Fang H
3. Groom L
4. Cheng A
5. Zhang W
6. Liu J
7. Wang X
8. Li K
9. Han P
10. Zheng M
11. Yin J
12. Wang W
13. Mattson MP
14. Kao JPY
15. Lakatta EG
16. Sheu S-S
17. Ouyang K
18. Chen J
19. Dirksen RT
20. Cheng H
(2008) Superoxide Flashes in Single Mitochondria
Cell 134:279–290.
https://doi.org/10.1016/j.cell.2008.06.017
- Google Scholar
1. Wang W
2. Gong G
3. Wang X
4. Wei-LaPierre L
5. Cheng H
6. Dirksen R
7. Sheu S-S
(2016a) Mitochondrial Flash: Integrative Reactive Oxygen Species and pH Signals in Cell and Organelle Biology
Antioxidants & Redox Signaling 25:534–549.
https://doi.org/10.1089/ars.2016.6739
- Google Scholar
1. Wang W
2. Zhang H
3. Cheng H
(2016b) Mitochondrial flashes: From indicator characterization to in vivo imaging
Methods 109:12–20.
https://doi.org/10.1016/j.ymeth.2016.06.004
- Google Scholar
1. Wang X
2. Zhang X
3. Huang Z
4. Wu D
5. Liu B
6. Zhang R
7. Yin R
8. Hou T
9. Jian C
10. Xu J
11. Zhao Y
12. Wang Y
13. Gao F
14. Cheng H
(2016c) Protons Trigger Mitochondrial Flashes
Biophysical Journal 111:386–394.
https://doi.org/10.1016/j.bpj.2016.05.052
- Google Scholar
1. Wang X
2. Zhang X
3. Wu D
4. Huang Z
5. Hou T
6. Jian C
7. Yu P
8. Lu F
9. Zhang R
10. Sun T
11. Li J
12. Qi W
13. Wang Y
14. Gao F
15. Cheng H
(2017) Mitochondrial flashes regulate ATP homeostasis in the heart
eLife 6:e23908.
https://doi.org/10.7554/eLife.23908
- Google Scholar
1. Wei-LaPierre L
2. Gong G
3. Gerstner BJ
4. Ducreux S
5. Yule DI
6. Pouvreau S
7. Wang X
8. Sheu S-S
9. Cheng H
10. Dirksen RT
11. Wang W
(2013) Respective Contribution of Mitochondrial Superoxide and pH to Mitochondria-targeted Circularly Permuted Yellow Fluorescent Protein (mt-cpYFP) Flash Activity
Journal of Biological Chemistry 288:10567–10577.
https://doi.org/10.1074/jbc.M113.455709
- Google Scholar
1. Winter J
2. Klumpe I
3. Heger J
4. Rauch U
5. Schultheiss H-P
6. Landmesser U
7. Dörner A
(2016) Adenine nucleotide translocase 1 overexpression protects cardiomyocytes against hypoxia via increased ERK1/2 and AKT activation
Cellular Signalling 28:152–159.
https://doi.org/10.1016/j.cellsig.2015.11.002
- Google Scholar
1. Yang L
2. Zhao K
3. Calingasan NY
4. Luo G
5. Szeto HH
6. Beal MF
(2009) Mitochondria targeted peptides protect against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine neurotoxicity
Antioxidants & Redox Signaling 11:2095–2104.
https://doi.org/10.1089/ars.2009.2445
- PubMed
- Google Scholar
1. Zhang H
2. Shang W
3. Zhang X
4. Gu J
5. Wang X
6. Zheng M
7. Wang Y
8. Zhou Z
9. Cao JM
10. Ji G
11. Zhang R
12. Cheng H
(2013) Β-adrenergic-stimulated L-type channel Ca²+ entry mediates hypoxic Ca²+ overload in intact heart
Journal of Molecular and Cellular Cardiology 65:51–58.
https://doi.org/10.1016/j.yjmcc.2013.09.002
- PubMed
- Google Scholar
1. Zhang M
2. Sun T
3. Jian C
4. Lei L
5. Han P
6. Lv Q
7. Yang R
8. Zhou X
9. Xu J
10. Hu Y
11. Men Y
12. Huang Y
13. Zhang C
14. Zhu X
15. Wang X
16. Cheng H
17. Xiong JW
(2015) Remodeling of mitochondrial flashes in muscular development and dystrophy in zebrafish
PLOS ONE 10:e0132567.
https://doi.org/10.1371/journal.pone.0132567
- PubMed
- Google Scholar
1. Zhang H
2. Wang P
3. Bisetto S
4. Yoon Y
5. Chen Q
6. Sheu SS
7. Wang W
(2017) A novel fission-independent role of dynamin-related protein 1 in cardiac mitochondrial respiration
Cardiovascular Research 113:160–170.
https://doi.org/10.1093/cvr/cvw212
- PubMed
- Google Scholar
1. Zhang H
2. Gong G
3. Wang P
4. Zhang Z
5. Kolwicz SC
6. Rabinovitch PS
7. Tian R
8. Wang W
(2018) Heart specific knockout of Ndufs4 ameliorates ischemia reperfusion injury
Journal of Molecular and Cellular Cardiology 123:38–45.
https://doi.org/10.1016/j.yjmcc.2018.08.022
- PubMed
- Google Scholar
1. Zorov DB
2. Filburn CR
3. Klotz LO
4. Zweier JL
5. Sollott SJ
(2000) Reactive oxygen species (ROS)-induced ROS release: a new phenomenon accompanying induction of the mitochondrial permeability transition in cardiac myocytes
The Journal of Experimental Medicine 192:1001–1014.
https://doi.org/10.1084/jem.192.7.1001
- PubMed
- Google Scholar

Article and author information

Author details

Huiliang Zhang

Department of Laboratory Medicine and Pathology, University of Washington, Seattle, United States

Contribution
Conceptualization, Resources, Data curation, Formal analysis, Funding acquisition, Investigation, Visualization, Methodology, Writing - original draft

Competing interests
No competing interests declared

"This ORCID iD identifies the author of this article:" 0000-0002-8967-1219
Nathan N Alder

Department of Molecular and Cell Biology, University of Connecticut, Storrs, United States

Contribution
Conceptualization, Resources, Methodology, Writing - review and editing

Competing interests
No competing interests declared
Wang Wang
1. Department of Laboratory Medicine and Pathology, University of Washington, Seattle, United States
2. Mitochondria and Metabolism Center, Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, United States
Contribution
Resources, Funding acquisition, Writing - review and editing

Competing interests
No competing interests declared
Hazel Szeto

Social Profit Network Research Lab, Alexandria LaunchLabs, New York, United States

Contribution
Writing - review and editing

Competing interests
is the inventor of SS-31 and founder of Stealth Biotherapeutics.
David J Marcinek

Department of Radiology, University of Washington, Seattle, United States

Contribution
Resources, Writing - review and editing

Competing interests
No competing interests declared
Peter S Rabinovitch

Department of Laboratory Medicine and Pathology, University of Washington, Seattle, United States

Contribution
Conceptualization, Resources, Funding acquisition, Writing - original draft, Project administration

For correspondence
petersr@uw.edu

Competing interests
No competing interests declared

"This ORCID iD identifies the author of this article:" 0000-0001-7169-3543

Funding

NIA (P01AG001751)

Peter S Rabinovitch

NIA (R56AG055114)

Peter S Rabinovitch

NHLBI (HL114760)

Wang Wang

NHLBI (HL137266)

Wang Wang

AHA (18EIA33900041)

Wang Wang

AHA (19CDA34660311)

Huiliang Zhang

Glenn/AFAR Foundation (Medical Research Postdoctoral Fellowship)

Huiliang Zhang

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Acknowledgements

We thank Drs. Mariya Sweetwyne, Ying Ann Chiao, Martin Brand, Michael MacCoss and Gaomin Feng for technical support and helpful discussions and the services of the WM Keck Microscopy Center at the University of Washington. SS-31 (elamipretide) was kindly provided by Stealth Biotherapeutics (Newton, MA).

This work was supported by NIA P01AG001751 and R56AG055114 to PSR, HL114760, HL137266 and AHA 18EIA33900041 to WW, and a Glenn Foundation for Medical Research Postdoctoral Fellowship and AHA 19CDA34660311 to HZ.

Ethics

Animal experimentation: All the animal procedures were approved by the Institutional Animal Care and Use Committee at the University of Washington and conform to the NIH guidelines (Guide for the care and use of laboratory animals). The use of mice and rats have been approved by IACUC under animal protocols 2174-23 and 2654-03.

Version history

Received: July 8, 2020
Accepted: December 1, 2020
Version of Record published: December 15, 2020 (version 1)

Copyright

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

Metrics

4,049

views
408

downloads
56

citations

Views, downloads and citations are aggregated across all versions of this paper published by eLife.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Open citations (links to open the citations from this article in various online reference manager services)

Mendeley

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

Huiliang Zhang
Nathan N Alder
Wang Wang
Hazel Szeto
David J Marcinek
Peter S Rabinovitch

(2020)

Reduction of elevated proton leak rejuvenates mitochondria in the aged cardiomyocyte

eLife 9:e60827.

https://doi.org/10.7554/eLife.60827

Categories and tags

Research organisms

Mouse
Rat

Share this article

Cite this article

SS-31 alleviates the excessive mitochondrial proton leak of cardiomyocytes from 24 mo old mice.

SS-31 restores the resistance of cardiomyocytes from old rat to proton entry into the mitochondrial matrix during external pH gradient stress.

ANT1 inhibitors restore resistance to proton leak of mitochondria in old rat cardiomyocytes.

SS-31 attenuates excessive mitoflash activity in aged rat cardiomyocytes.

SS-31 reverses the increased speed of mitochondrial permeability transition pore (mPTP) opening in aged mouse cardiomyocytes.

SS-31 interacts with ANT1 and stabilizes the ATP synthasome in the old mouse heart mitochondria.

Schematic of the mechanism of SS-31 protection of proton leak and rejuvenation of mitochondrial function.

Author details

Huiliang Zhang

Contribution

Competing interests

Nathan N Alder

Contribution

Competing interests

Wang Wang

Contribution

Competing interests

Hazel Szeto

Contribution

Competing interests

David J Marcinek

Contribution

Competing interests

Peter S Rabinovitch

Contribution

For correspondence

Competing interests

Downloads (link to download the article as PDF)

Open citations (links to open the citations from this article in various online reference manager services)

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

Categories and tags

Research organisms

Further reading