Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Antimicrobial resistance and resistance transfer of Vibrio parahaemolyticus and Morganella morganii from commercial fisheries products

시판 수산물에서 분리한 Vibrio parahaemolyticus와 Morganella morganii의 항균제 내성과 내성 전이

  • Lee, Ye Ji (Department of Aqualife Medicine, Chonnam National University) ;
  • Kim, Eunheui (Department of Aqualife Medicine, Chonnam National University)
  • 이예지 (전남대학교 수산생명의학과) ;
  • 김은희 (전남대학교 수산생명의학과)
  • Received : 2019.11.06
  • Accepted : 2019.12.09
  • Published : 2019.12.31
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Abstract

The purpose of this study was to investigate the antimicrobial resistance and resistance transfer of Vibrio parahaemolyticus and Morganella morganii isolated from fish products purchased from fish markets in Yeosu April - December 2017. These bacteria were identified by biochemical test and PCR results, and the transfer of antimicrobial resistance was confirmed by the broth mating method. To isolate the transconjugants formed during conjugation, TSA medium containing 50 ㎍/ml of ampicillin (AMP), and 150 ㎍/ml of streptomycin (SM) or 30 ㎍/ml of oxytetracycline (OT) was used. M. morganii isolates showed low susceptibility to AMP, amoxicillin (AML), and colistin (CT), erythromycin, OT, and tetracycline, compared to V. parahaemolyticus resistance to AMP, AML, and CT. The conjugation of V. parahaemolyticus or M. morganii with Escherichia coli resulted in the separation of V. parahaemolyticus and M. morganii showing SM resistance as transconjugants. Meanwhile, Edwardsiella tarda transconjugants showing AMP and AML resistance were obtained from the broth mating of V. parahaemolyticus and E. tarda. But the transfer of the VPA0477 which is a β-lactamase gene of V. parahaemolyticus was not confirmed. These results suggest that resistance transfer between pathogenic bacteria is bidirectional and progresses in a wide variety of patterns.

본 연구에서는 2017년 4월부터 12월 사이에 여수수산시장에서 구입한 수산물로부터 분리된 Vibrio parahaemolyticus와 Morganella morganii의 내성특성을 알아보고 장내세균인 Escherichia coli와 어류 병원균인 Edwardsiella tarda와의 내성전이 가능성을 알아보았다. Broth mating 법으로 실시한 conjugation에서 형성된 transconjugants를 분리하기 위하여 ampicillin(AMP) 50 ㎍/ml와 streptomycin (SM) 150 ㎍/ml 또는 oxytetracycline(OT) 30 ㎍/ml이 첨가된 TSA배지를 사용하였다. AMP, amoxicillin (AML), colistin(CT)에 낮은 감수성을 보였던 V. parahaemolyticus와 달리 M. morganii는 AMP, AML, CT, erythromycin, OT, tetracyclin등에 감수성이 낮았다. V. parahaemolyticus와 M. morganii를 E. coli와 conjugation을 실시한 결과 transconjugants인 E. coli는 분리되지 않은 반면 SM내성을 획득한 V. parahaemolyticus와 M. morganii가 분리되었다. 한편 V. parahaemolyticus와 E. tarda의 broth mating에서는 AMP와 AML 내성을 획득한 E. tarda transconjugant가 분리되었다. 그러나 V. parahaemolyticus의 β-lactamase 유전자인 VPA 0477의 전이는 확인되지 않았으며, E. tarda의 내성이 전달된 V. parahaemolyticus나 M. morganii는 검출되지 않았다. 이와 같은 결과는 생물 위해성 세균들 간의 내성전이가 양방향성이며 매우 다양한 패턴으로 진행되고 있음을 시사한다.

Keywords

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