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Measurement of Normal Spring Constant of Colloidal Probes for Atomic Force Microscope

원자 현미경용 콜로이드 탐침 수직 스프링 상수 측정

  • Kim, Dae-Hyun (Graduate School of NID Fusion Technology, Seoul National University of Science and Technology) ;
  • Kim, Min-Seok (Korea Research Institute of Standards and Science) ;
  • Hahn, Junhee (Korea Research Institute of Standards and Science) ;
  • Ahn, Hyo-Sok (College of Business and Technology, Seoul National University of Science and Technology)
  • 김대현 (서울과학기술대학교 NID융합기술대학원) ;
  • 김민석 (한국표준과학연구원) ;
  • 한준희 (한국표준과학연구원) ;
  • 안효석 (서울과학기술대학교 기술경영융합대학)
  • Received : 2012.05.17
  • Accepted : 2012.07.20
  • Published : 2012.10.31

Abstract

A modified thermal noise method was proposed to measure the normal spring constants of the colloidal probes for an atomic force microscope. We used commercial tipless cantilevers (length 150, width 30, nominal k 7.4 N/m) and borosilicate spheres with a diameter of 20 to fabricate colloidal probes. The inverse optical lever sensitivity of both the tipless cantilever and colloidal probes were used to measure the normal spring constant of the colloidal probes. We confirmed the accuracy and usefulness of our method by comparing the measurement results with those obtained using the nanoforce calibrator (NFC), which reportedly has an uncertainty of 1.00%. The modified thermal method showed a good agreement (~10% difference) with the NFC, allowing us to conclude that the modified thermal method could be employed for the effective measurement of the normal spring constants of colloidal probes.

Keywords

References

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