RP HPLC Method Development for Simultaneous Estimation of Etoricoxib and Thiocolchicoside
Anchal Sharma *
School of Pharmacy, Abhilashi University Chailchowk, India.
Amit Chaudhary
School of Pharmacy, Abhilashi University Chailchowk, India.
Bhupendra Singh
Abhilashi College of Pharmacy, Nerchowk, India.
*Author to whom correspondence should be addressed.
Abstract
A simple, specific and accurate reverse phase high performance liquid chromatography (RP-HPLC) method was developed and validated for simultaneous estimation of Etoricoxib and Thiocolchicoside in bulk and solid dosage form. Separation was achieved by Zorbax C-18 analytical column having dimension (250mm * 4.6 mm i.d 5.0 µm) using methanol and water (60:40) as mobile phase and flow rate was 0.7ml/min. The detection was carried out at 283 nm wavelength using UV detector. The total chromatographic sample time per analysis was about 14.0 minutes with thiocolchicoside eluted at retention time 3.523 min and etoricoxib eluted at retention time 9.627 min. The method was validated for accuracy, precision, specificity, rapid, reliable and reproducible. LOD and LOQ value for etoricoxib and thiocolchicoside were found to be 0.332, 0.996 ppm and 0976, 0.928 ppm respectively. Regression Equation for Etoricoxib was y = 0.006x + 0.149 and regression equation for thiocolchicoside was Y = 0.030x + 0.086. As the run time was increased the retention time was decreased, so the method is simple and economical and can be adopted by regular quality control in industries and also in research laboratories.
Keywords: Etoricoxib, thiocolchicoside, RP-HPLC, validation