Childrens intestinal microbiocenosis at different stages of HIV infection

Zakharova Yu.V., Bykov А.С., Nurtazina A.Y., Markovskaya A.A., Afanasiev S.S., Nesvizhsky Y.V., Afanasiev M.S.

Kemerovo State Medical University, Kemerovo
G.N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology, Moscow
Ministry of Health and Social Development of the Russian Federation I.M. Sechenov First Moscow State Medical University, Moscow

The aim is to assess the state of intestinal microbiocenosis in HIV-infected children at different stages of the disease.

Materials and methods: intestinal microbiocenosis was studied by quantitative bacteriological method in 85 HIV-infected children aged 24±2 months. Stage 2 b of HIV infection was reported in 45 (52.9%), stage 2 In 19 (22.4%) children, stage 3 in 13 (15.3%), stage 4 in 8 (9.4%). All children received antiretroviral therapy.

Results: children under 2 B stage of HIV infection with a high content of bifidobacteria, lactobacilli, enterococci has high frequency and levels of the vegetated bacteria of the genus Klebsiella (37.3%; 8 (6; 9) lg CFU/g) Escherichia coli hly+ (35.3%; 7 (5; 8) lg CFU/g) Staphylococcus aureus (29.4%; 5 (3; 6) lg CFU/g), S. epidermidis (15.7%; 5 (3; 6) lg CFU/g), the fungi of the genus Candida (58.9%; 5 (3; 6) lg CFU/g). At stage 2 V, the population level of bifidobacteria decreased to 7 (6; 8) lg CFU/g, the frequency of colonization by Candida increased by 1.5 times, which reached an average concentration of 6 (5; 8) lg CFU/g. In children with stage 3 HIV infection has registered an increase of 2 lg of quantitative levels of bifidobacteria, reduced to 30.8 % detection frequency of Candida, the average was 3 (2; 4) lg CFU/g. Klebsiella spp. and E. coli lac-were found in 30.8% and 23.1% of individuals, respectively. In stage 4 of HIV infection, the frequency of bifidobacteria and lactobacilli was 87.5%, the titers were below the age norms by 1-2 lg. 2 times increased frequency of colonization of mucosa by E. coli hly+, in 4 times – with Candida.

Conclusion: changes in intestinal microbiocenosis are specific at each stage of HIV infection, which should be taken into correcting.